Abstract:
:The Wnt signalling pathway plays a crucial role in the development of the nervous system. This signalling cascade is initiated upon binding of the secreted Wnt ligand to a member of the family of frizzled receptors. In the present study, we analysed the presence of frizzled-4 in the enteric nervous system of human infants. Frizzled-4 could be identified by immunohistochemistry in a subpopulation of enteric neuronal and glial cells in the small and large intestine. Detection of frizzled-4 in the tunica muscularis by RT-PCR confirmed this receptor's expression on the mRNA level. Interestingly, we observed distinct cell populations that co-expressed frizzled-4 with the intermediate filament protein nestin and the neurotrophin receptor p75(NTR), which have been reported to be expressed in neural progenitor cells. Flow cytometry analysis revealed that 60% of p75(NTR) positive cells of the tunica muscularis were positive for frizzled-4. Additionally, in pathological samples of Hirschsprung's disease, the expression of this Wnt receptor correlated with the number of myenteric ganglion cells and decreased from normoganglionic to aganglionic areas of large intestine. The expression pattern of frizzled-4 indicates that this Wnt receptor could be involved in postnatal development and/or function of the enteric nervous system.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Nothelfer K,Obermayr F,Belz N,Reinartz E,Bareiss PM,Bühring HJ,Beschorner R,Just Ldoi
10.1155/2016/9076823subject
Has Abstractpub_date
2016-01-01 00:00:00pages
9076823eissn
1687-966Xissn
1687-9678journal_volume
2016pub_type
杂志文章abstract::Evidences have supported the pivotal roles of stem cells in mammary gland development. Many molecular markers have been identified to characterize mammary stem cells. Cellular fate mapping of mammary stem cells by lineage tracing has put unprecedented insights into the mammary stem cell biology, which identified two s...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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abstract::Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in c...
journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章
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abstract::Osteoporosis, femoral head necrosis, and congenital bone defects are orthopedic disorders characterized by reduced bone generation and insufficient bone mass. Bone regenerative therapy primarily relies on the bone marrow mesenchymal stem cells (BMSCs) and their ability to differentiate osteogenically. Icariin (ICA) is...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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abstract:Objective:To investigate the effects of mesenchymal stem cells (MSCs) coated by the extracellular matrix (ECM) on wound healing in diabetic rats. Methods:Mesenchymal stem cells were cocultured with ECM. Cell viabilities were evaluated using MTT assay. The diabetes model was established using both STZ and high-glucose/...
journal_title:Stem cells international
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abstract::Pulmonary emphysema is a respiratory condition characterized by alveolar destruction that leads to airflow limitation and reduced lung function. Although with extensive research, the pathophysiology of emphysema is poorly understood and effective treatments are still missing. Evidence suggests that mesenchymal stem ce...
journal_title:Stem cells international
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abstract::Coronary revascularization remains the standard treatment for obstructive coronary artery disease and can be accomplished by either percutaneous coronary intervention (PCI) or coronary artery bypass graft surgery. Considerable advances have rendered PCI the most common form of revascularization and improved clinical o...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/128732
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abstract::Cord blood transplantation (CBT) is an attractive alternative therapy in adult patients with advanced hematological malignancies in whom matched donors are unavailable. However, the risk of complications, especially infections, post-CBT increases the mortality rates in these patients. Although the incidence of acute a...
journal_title:Stem cells international
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/1690896
更新日期:2016-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are multipotent stem cells characterized by self-renewal, production of clonal cell populations, and multilineage differentiation. They exist in nearly all tissues and play a significant role in tissue repair and regeneration. Additionally, MSCs possess wide immunoregulatory properties vi...
journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/4214281
更新日期:2019-11-12 00:00:00
abstract::Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n = 4) or allogeneic cultu...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/391274
更新日期:2014-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3579283
更新日期:2017-01-01 00:00:00
abstract::Aged tendon-derived stem/progenitor cells (TSPCs) lead to age-related tendon disorders and impair tendon healing. However, the underlying molecular mechanisms of TSPC aging remain largely unknown. Here, we investigated the role of connective tissue growth factor (CTGF) in TSPC aging. CTGF protein and mRNA levels were ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/6257537
更新日期:2019-11-11 00:00:00
abstract::Exosomes derived from cancer cells can affect various functions of mesenchymal stem cells (MSCs) via conveying microRNAs (miRs). miR-21 and miR-146a have been demonstrated to regulate MSC proliferation and transformation. Interleukin-6 (IL-6) secreted from transformed MSCs in turn favors the survival of multiple myelo...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/9012152
更新日期:2017-01-01 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2015/471216
更新日期:2015-01-01 00:00:00
abstract::Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, characterized by progressive cartilage degradation, subchondral bone remodeling, synovitis, and chronic pain. Due to the limited self-healing capacity in condylar cartilage, traditional clinical treatments have limited symptom-modifying and str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/5979741
更新日期:2017-01-01 00:00:00
abstract::In contrast to the pluripotent embryonic stem cells (ESCs) which are able to give rise to all cell types of the body, mammalian adult stem cells (ASCs) appeared to be more limited in their differentiation potential and to be committed to their tissue of origin. Recently, surprising new findings have contradicted centr...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/589139
更新日期:2013-01-01 00:00:00
abstract:Purpose:To investigate the efficacy of recombinant human collagen type I (RHC I) and collagen-like peptide (CLP) hydrogels as alternative carrier substrates for the cultivation of limbal epithelial stem cells (LESC) under xeno-free culture conditions. Methods:Human LESC were cultivated on seven different collagen-deri...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7867613
更新日期:2019-04-01 00:00:00
abstract::Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cel...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/250740
更新日期:2013-01-01 00:00:00
abstract::Due to their immunomodulatory and chemotactic properties, hMSC are being explored to treat immune-related diseases. For their use in human therapies, it is necessary to culture hMSC in xeno-free conditions. In this study, the impact that a xeno-free medium based on a human plasma derivate has on these properties was a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/2185351
更新日期:2017-01-01 00:00:00
abstract::Three-dimensional (3D) cell culture has been reported to increase the therapeutic potentials of mesenchymal stem cells (MSCs). However, the action mechanisms of 3D MSCs vary greatly and are far from being thoroughly investigated. In this study, we aimed to investigate the therapeutic effects of 3D spheroids of human a...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/4626073
更新日期:2016-01-01 00:00:00
abstract::Endometriosis is an inflammatory disease with predominance of immunosuppressive M2 macrophages in the pelvic cavity that could be involved in the pathology through support and immune escape of ectopic lesions. Mesenchymal stromal cells (MSC) are found in ectopic lesions, and MSC from nonendometriosis sources are known...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3215962
更新日期:2017-01-01 00:00:00
abstract::Aging, chronic inflammation, and environmental insults play an important role in a number of disease processes through alterations of the epigenome. In this review we explore how age-related changes in the epigenetic landscape can affect heterogeneity within the haematopoietic stem cell (HSC) compartment and the deriv...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/5797521
更新日期:2016-01-01 00:00:00
abstract::Tumor microenvironment has been implicated in tumor development and progression. As a three-dimensional tumor microenvironment model, air liquid interface (ALI) organoid culture from oncogene transgenic mouse gastrointestinal tissues was recently produced. However, ALI organoid culture system from tissues of colorecta...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/7053872
更新日期:2016-01-01 00:00:00
abstract:Aim:Few haematopoietic stem cells (HSCs) injected systemically for therapeutic purposes actually reach sites of injury as the vast majority become entrapped within pulmonary capillaries. One promising approach to maintain circulating HSC numbers would be to separate subpopulations with smaller size and/or greater defor...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/8540706
更新日期:2019-09-05 00:00:00
abstract:Objective:Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/5128128
更新日期:2020-02-14 00:00:00
abstract::Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of D...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/2157451
更新日期:2018-05-09 00:00:00
abstract::Radiation is the most potent mode of cancer therapy; however, resistance to radiation therapy results in tumor relapse and subsequent fatality. The cancer stem cell (CSC), which has better DNA repair capability, has been shown to contribute to tumor resistance and is an important target for treatment. Signaling molecu...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/5785786
更新日期:2016-01-01 00:00:00