Abstract：
:Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The in vivo application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce muscle regeneration, was evaluated in a rat myectomy model. Two commercially available vehicles and a spherical hydrogel developed by our research group were used. The treated groups obtained interesting results in terms of muscle regeneration, both in the histological and in the functional assessments. A less evident scar tissue, demonstrated by collagen type I quantification, was present in the muscles treated with HMSCs or their CM. In terms of the histological evaluation performed by ISO 10993-6 scoring, it was observed that HMSCs apparently have a long-term negative effect, since the groups treated with CM presented better scores. CM could be considered an alternative to the in vivo transplantation of these cells, as it can benefit from the local tissue response to secreted molecules with similar results in terms of muscular regeneration. Searching for an optimal vehicle might be the key point in the future of skeletal muscle tissue engineering.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Pereira T,Armada-da Silva PA,Amorim I,Rêma A,Caseiro AR,Gärtner A,Rodrigues M,Lopes MA,Bártolo PJ,Santos JD,Luís AL,Maurício ACdoi
10.1155/2014/376918subject
Has Abstractpub_date
2014-01-01 00:00:00pages
376918eissn
1687-966Xissn
1687-9678journal_volume
2014pub_type
杂志文章abstract：:Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title：Stem cells international
pub_type： 杂志文章,评审
doi：10.1155/2016/2953727
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journal_title：Stem cells international
pub_type： 杂志文章
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journal_title：Stem cells international
pub_type： 杂志文章
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
pub_type： 杂志文章
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更新日期：2020-06-30 00:00:00
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journal_title：Stem cells international
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Stem cells international
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Stem cells international
pub_type： 杂志文章
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journal_title：Stem cells international
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Stem cells international
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Stem cells international
pub_type： 杂志文章
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更新日期：2019-12-17 00:00:00
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
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journal_title：Stem cells international
pub_type： 杂志文章,评审
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journal_title：Stem cells international
pub_type： 杂志文章,评审
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journal_title：Stem cells international
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abstract：:Three-dimensional (3D) cell culture has been reported to increase the therapeutic potentials of mesenchymal stem cells (MSCs). However, the action mechanisms of 3D MSCs vary greatly and are far from being thoroughly investigated. In this study, we aimed to investigate the therapeutic effects of 3D spheroids of human a...
journal_title：Stem cells international
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journal_title：Stem cells international
pub_type： 杂志文章,评审
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journal_title：Stem cells international
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journal_title：Stem cells international
pub_type： 杂志文章,评审
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