Abstract:
:A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA. First, we showed that heat-treated BAp films can significantly promote hDPSC adhesion and proliferation. Moreover, hDPSCs, while initially maintaining the typical fibroblast-like morphology and stemness surface markers, later started expressing osteogenic markers such as Runx-2 and OSX. Noteworthy, when cultured in an osteogenic medium on annealed BAp films, hDPSCs were also able to reach a more mature and terminal commitment, with respect to HA and as-deposited films. Our findings suggest that annealed BAp films not only preserve the typical biological properties of stemness of, hDPSCs but also improve their ability of osteogenic commitment.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Bianchi M,Pisciotta A,Bertoni L,Berni M,Gambardella A,Visani A,Russo A,de Pol A,Carnevale Gdoi
10.1155/2017/3579283subject
Has Abstractpub_date
2017-01-01 00:00:00pages
3579283eissn
1687-966Xissn
1687-9678journal_volume
2017pub_type
杂志文章abstract::Stem cell therapy with neural precursor cells (NPCs) has the potential to improve neuroregeneration after spinal cord injury (SCI). Unfortunately, survival and differentiation of transplanted NPCs in the injured spinal cord remains low. Growth factors have been successfully used to improve NPC transplantation in anima...
journal_title:Stem cells international
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abstract::Efficient ex vivo expansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition. Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported. Here, we describe a simplified 3D hanging drop model f...
journal_title:Stem cells international
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abstract::Intervertebral disc degeneration (IVD) is a frequent pathological condition. Conservative management often fails, and patients with IVD degeneration may require surgical intervention. Several treatment strategies have been proposed, although only surgical discectomy and arthrodesis have been proved to be predictably e...
journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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abstract::Faced with the end stage of heart disease, the current treatments only slow worsening of heart failure. Stem cells have the potential of self-renewal and differentiation. It is expected to replace and repair damaged myocardium. But many clinical trials have shown that the stem cell therapy of heart failure is modest o...
journal_title:Stem cells international
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abstract::Beer is one of the most consumed alcoholic beverages in the world, rich in chemical compounds of natural origin with high nutritional and biological value. It is made up of water, barley malt, hops, and yeast. The main nutrients are carbohydrates, amino acids, minerals, vitamins, and other compounds such as polyphenol...
journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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更新日期:2018-08-05 00:00:00
abstract::Human liver stem cells (HLSCs) are a mesenchymal stromal cell-like population resident in the adult liver. Preclinical studies indicate that HLSCs could be a good candidate for cell therapy. The aim of the present study was to evaluate the immunogenicity and the immunomodulatory properties of HLSCs on T-lymphocytes, n...
journal_title:Stem cells international
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doi:10.1155/2016/8468549
更新日期:2016-01-01 00:00:00
abstract::Vitamin D (Vit D) by means of its biological active form, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), has a protective effect on the skeleton by acting on calcium homeostasis and bone formation. Furthermore, Vit D has a direct effect on mesenchymal stem cells (MSCs) in stimulating their osteogenic differentiation. In thi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/6958713
更新日期:2018-02-28 00:00:00
abstract:Background:Ly6Chigh monocytes are inflammatory cells that accumulate in an infarcted myocardium, and Ly6Clow monocytes are believed to be reparative and curb myocardial remodeling. NR4A1 is a novel target for modulating the inflammatory phenotype of monocytes during atherogenesis. Objectives:We aimed to investigate wh...
journal_title:Stem cells international
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doi:10.1155/2020/2460158
更新日期:2020-01-13 00:00:00
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journal_title:Stem cells international
pub_type: 杂志文章,评审
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journal_title:Stem cells international
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journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/3123961
更新日期:2018-04-29 00:00:00
abstract::A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) causing lethal acute respiratory disease emerged in December 2019. The World Health Organization named this disease "COVID-19" and declared it a pandemic on March 11, 2020. Many studies have shown that mesenchymal stem cells (MSCs) and their exosomes (...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/8835986
更新日期:2020-09-24 00:00:00
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journal_title:Stem cells international
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doi:10.1155/2017/5251313
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journal_title:Stem cells international
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doi:10.1155/2019/8472712
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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journal_title:Stem cells international
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abstract::Dental pulp stem cells (DPSCs) are ideal seed cells for the regeneration of dental tissues. However, DPSC senescence restricts its clinical applications. Metformin (Met), a common prescription drug for type 2 diabetes, is thought to influence the aging process. This study is aimed at determining the effects of metform...
journal_title:Stem cells international
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更新日期:2021-01-06 00:00:00
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journal_title:Stem cells international
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