Abstract:
Background:Cancer stem cell (CSC) promotes angiogenesis which plays an important role in tumor occurrence, growth, and metastasis. Accurately, quantifying the tumor vasculature can help understanding CSC characteristics and improve cancer diagnosis, therapy planning, and evaluation. The objective of this study is to present a method for improved angiogenesis assessment. Methods:We proposed a three-dimensional microvessel density (3D MVD) to evaluate tumor angiogenesis and tested it in animal models. Six male Balb/c nude mice were divided into normal group and tumor group. The mice in tumor group were orthotopically implanted human gastric cancer, cell line BGC-823. The phase-contrast images were collected at Shanghai Synchrotron Radiation Facility BL13W beamline, which has much higher soft-tissue contrast and spatial resolution than conventional X-ray. After volume reconstruction and vessel extraction, the 3D models of the angiogenesis were established for MVD calculation. Results:The results showed that the proposed 3D MVD is positively correlated with the pathological changes of the microvessels. It took the advantage of high resolution of the phase-contrast imaging and added three-dimensional information to the existing MVD measure. Conclusions:Our study presents a feasible approach for better understanding of tumor angiogenesis. It may provide doctors and scientists a better tool for cancer investigation and improving medical outcomes.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Zhang L,Zheng F,Peng Z,Hu Z,Yang Zdoi
10.1155/2018/7813729subject
Has Abstractpub_date
2018-04-03 00:00:00pages
7813729eissn
1687-966Xissn
1687-9678journal_volume
2018pub_type
杂志文章abstract::Whether liver epithelial cells contribute to the development of hepatic scarring by undergoing epithelial-to-mesenchymal transition (EMT) is a controversial issue. Herein, we revisit the concept of EMT in cholangiopathies, a group of severe hepatic disorders primarily targeting the bile duct epithelial cell (cholangio...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/2953727
更新日期:2016-01-01 00:00:00
abstract::A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/3579283
更新日期:2017-01-01 00:00:00
abstract::Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development. A new way to maintain cESCs for a long period in vitro still remains unexplored. We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of D...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/2157451
更新日期:2018-05-09 00:00:00
abstract::Delayed administration of kallikrein after cerebral infarction can improve neurological function. However, the appropriate kallkrein treatment time after ischemic stroke has not been illuminated. In this study, we compared the long-term outcome among three kallikrein therapeutic regimens starting at different time poi...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2018/1706982
更新日期:2018-04-05 00:00:00
abstract::Aging of population brings related social problems, such as muscle attenuation and regeneration barriers with increased aging. Muscle repair and regeneration depend on muscle stem cells (MuSCs). Obstructive sleep apnea (OSA) rises in the aging population. OSA leads to hypoxia and upper airway muscle injury. However, l...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8412598
更新日期:2020-03-04 00:00:00
abstract::Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n = ...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/452102
更新日期:2012-01-01 00:00:00
abstract::Articular cartilage defects have been addressed by using multiple strategies. In the last two decades, promising new strategies by using assorted scaffolds and cell sources to induce tissue regeneration have emerged, such as autologous chondrocyte implantation (ACI) and mesenchymal stem cell implantation (MSCI). Howev...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9201492
更新日期:2016-01-01 00:00:00
abstract::Alopecia is caused by a variety of factors which affect the hair cycle and decrease stem cell activity and hair follicle regeneration capability. This process causes lower self-acceptance, which may result in depression and anxiety. However, an early onset of androgenic alopecia is associated with an increased inciden...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2018/1049641
更新日期:2018-08-05 00:00:00
abstract::Cardiovascular diseases (CVDs), including atherosclerosis, stroke, and myocardial infarction, is a major cause of death worldwide. In aspects of cell therapy against CVD, it is generally accepted that endothelial progenitor cells (EPCs) are potent neovascular modulators in ischemic tissues. In response to ischemic inj...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/8340257
更新日期:2016-01-01 00:00:00
abstract::Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid. Study Design. Animal experiment. Method. We used 74 New Zealand...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/9010279
更新日期:2016-01-01 00:00:00
abstract::Intestinal stem cells are located at the base of the crypts and are surrounded by a complex structure called niche. This environment is composed mainly of epithelial cells and stroma which provides signals that govern cell maintenance, proliferation, and differentiation. Understanding how the niche regulates stem cell...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2017/8387297
更新日期:2017-01-01 00:00:00
abstract::The concerted action of ion channels and pumps establishing a resting membrane potential has been most thoroughly studied in the context of excitable cells, most notably neurons, but emerging evidences indicate that they are also involved in controlling proliferation and differentiation of nonexcitable somatic stem ce...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/816049
更新日期:2012-01-01 00:00:00
abstract::Urine has become the source of choice for noninvasive renal epithelial cells and renal stem cells which can be used for generating induced pluripotent stem cells. The aim of this study was to generate a 3D nephrogenic progenitor cell model composed of three distinct cell types-urine-derived SIX2-positive renal progeni...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/3298432
更新日期:2019-05-02 00:00:00
abstract::Histone deacetylase inhibitors (HDACi) are small molecules that have important and pleiotropic effects on cell homeostasis. Under distinct developmental conditions, they can promote either self-renewal or differentiation of embryonic stem cells. In addition, they can promote directed differentiation of embryonic and t...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2012/184154
更新日期:2012-01-01 00:00:00
abstract::In recent years several genes have linked amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) as a spectrum disease; however little is known about what triggers their onset. With the ability to generate patient specific stem cell lines from somatic cells, it is possible to model disease without the n...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/9279516
更新日期:2016-01-01 00:00:00
abstract::Myocarditis is a common inflammatory cardiomyopathy, associated with cardiomyocyte apoptosis, which can lead to chronic left ventricular dysfunction. Under conventional heart failure therapy, inflammatory cardiomyopathy typically has a progressive course, indicating a need for alternative therapeutic strategies to imp...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/353097
更新日期:2013-01-01 00:00:00
abstract::Recent studies indicate important roles for long noncoding RNAs (lncRNAs) as essential regulators of gene expression. However, the specific roles of lncRNAs in stenotic lesions of arteriovenous fistula (AVF) failure are still largely unknown. We first analyzed the expression profiles of lncRNAs in human stenosed and n...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2017/4252974
更新日期:2017-01-01 00:00:00
abstract::The mesenchymal stem cells (MSCs) are known as highly plastic stem cells and can differentiate into specialized tissues such as adipose tissue, osseous tissue, muscle tissue, and nervous tissue. The differentiation of mesenchymal stem cells is very important in regenerative medicine. Their differentiation process is r...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2020/3763069
更新日期:2020-08-01 00:00:00
abstract::Podocyte death and regeneration are major topics in kidney research but remain controversial. Data obtained in humans demonstrate the existence of cells sited along Bowman's capsule that behave as podocyte progenitors in vitro and in in vivo mouse models of podocyte injury xenotrasplanted with this human-derived popul...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2015/678347
更新日期:2015-01-01 00:00:00
abstract::Asherman's Syndrome (AS) is an uncommon, acquired, and refractory gynecological disorder. Current treatment was still limited, and stem cell-based therapy has been proposed as a novel strategy for management of AS. Here, we conducted a meta-analysis of self-controlled clinical trials to assess the effectiveness and sa...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8820538
更新日期:2020-12-19 00:00:00
abstract::Dental pulp stem cells (DPSCs) are ideal seed cells for the regeneration of dental tissues. However, DPSC senescence restricts its clinical applications. Metformin (Met), a common prescription drug for type 2 diabetes, is thought to influence the aging process. This study is aimed at determining the effects of metform...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2021/6616240
更新日期:2021-01-06 00:00:00
abstract::Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n = 4) or allogeneic cultu...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/391274
更新日期:2014-01-01 00:00:00
abstract::Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM) in vivo. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex str...
journal_title:Stem cells international
pub_type: 杂志文章,评审
doi:10.1155/2016/6397820
更新日期:2016-01-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder. Current targeted therapies designed to inhibit the tyrosine kinase activity of the BCR-ABL oncoprotein have made a significant breakthrough in the treatment of CML patients. However, CML remains a chronic disease that a patient must manage for lif...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/724360
更新日期:2013-01-01 00:00:00
abstract::Beer is one of the most consumed alcoholic beverages in the world, rich in chemical compounds of natural origin with high nutritional and biological value. It is made up of water, barley malt, hops, and yeast. The main nutrients are carbohydrates, amino acids, minerals, vitamins, and other compounds such as polyphenol...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/8835813
更新日期:2020-10-10 00:00:00
abstract::The lung is a radiosensitive organ, which imposes limits on the therapeutic dose in thoracic radiotherapy. Irradiated alveolar epithelial cells promote radiation-related pneumonitis and fibrosis. However, the role of lung stem cells (LSCs) in the development of radiation-induced lung injury is still unclear. In this s...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2020/6312053
更新日期:2020-09-29 00:00:00
abstract::Fifty-six aphaereses were performed in 23 pediatric patients with malignant hematological and solid tumors, following three different protocols for PBPC mobilization and distributed as follows: A: seventeen mobilized with 4 g/m(2) of cyclophosphamide (CFA) and 10 μg/kg/day of granulocyte colony stimulating factor (G-C...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2016/4078215
更新日期:2016-01-01 00:00:00
abstract::Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The in vivo application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated wit...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2014/376918
更新日期:2014-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2013/507301
更新日期:2013-01-01 00:00:00
abstract::The migration of administered mesenchymal stem cells (MSCs) to sites of injury via the bloodstream has been demonstrated. However, the underlying mechanisms of umbilical cord MSC adhesion to endothelial cells during transendothelial migration are still unclear. In this study, our data showed that IL-1β induced LFA-1 e...
journal_title:Stem cells international
pub_type: 杂志文章
doi:10.1155/2019/7267142
更新日期:2019-12-27 00:00:00