Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD).

Abstract:

:An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Wang Y,Zhang J,Lei Y,Zhao J

doi

10.1016/j.scr.2018.11.008

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

265-268

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(18)30277-0

journal_volume

33

pub_type

杂志文章
  • Derivation of LIF-independent mouse iPS cells with modified Oct4.

    abstract::It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, ...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2015.08.005

    authors: Hirai H,Firpo M,Kikyo N

    更新日期:2015-09-01 00:00:00

  • The granulocyte macrophage-colony stimulating factor surface modified MB49 bladder cancer stem cells vaccine against metastatic bladder cancer.

    abstract::The MB49 bladder cancer cell vaccine was effective against bladder cancer in the mice model in previous studies. However, part of the tumors regrew as the vaccine could not eliminate the cancer stem cells (CSCs). MB49 bladder cancer stem cells (MCSCs) were isolated by a combination of the limited dilution method and t...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2014.04.006

    authors: Zhu YT,Zhao Z,Fu XY,Luo Y,Lei CY,Chen W,Li F,Pang SY,Chen SS,Tan WL

    更新日期:2014-07-01 00:00:00

  • Embryonic stem cell-derived motoneurons provide a highly sensitive cell culture model for botulinum neurotoxin studies, with implications for high-throughput drug discovery.

    abstract::Botulinum neurotoxins (BoNTs) inhibit cholinergic synaptic transmission by specifically cleaving proteins that are crucial for neurotransmitter exocytosis. Due to the lethality of these toxins, there are elevated concerns regarding their possible use as bioterrorism agents. Moreover, their widespread use for cosmetic ...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2011.01.002

    authors: Kiris E,Nuss JE,Burnett JC,Kota KP,Koh DC,Wanner LM,Torres-Melendez E,Gussio R,Tessarollo L,Bavari S

    更新日期:2011-05-01 00:00:00

  • Establishment of an induced pluripotent stem cell line from a retinitis pigmentosa patient with compound heterozygous CRB1 mutation.

    abstract::The human iPSC line LEIi006-A was generated from dermal fibroblasts from a patient with retinitis pigmentosa using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. The iPSC cells carry compound heterozygous mutations (c.1892A > G and c.2548G > A) in the CRB1 gene. LEI...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2018.08.001

    authors: Zhang X,Zhang D,Chen SC,Lamey T,Thompson JA,McLaren T,De Roach JN,Chen FK,McLenachan S

    更新日期:2018-08-01 00:00:00

  • Long-term microcarrier suspension cultures of human embryonic stem cells.

    abstract::The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on ma...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2009.02.005

    authors: Oh SK,Chen AK,Mok Y,Chen X,Lim UM,Chin A,Choo AB,Reuveny S

    更新日期:2009-05-01 00:00:00

  • Generation of induced pluripotent stem cell (iPSC) line from a patient with triple negative breast cancer with hereditary exon 17 deletion of BRCA1 gene.

    abstract::BRCA1 germline mutation confers hereditary predisposition for breast and ovarian cancer. To understand the physiopathology of mammary and ovarian epithelial cancer transformation, and to identify early driver molecular events, we have generated an iPSC line from a patient carrying a germline exon 17 deletion in BRCA1 ...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2017.09.003

    authors: Griscelli F,Oudrhiri N,Feraud O,Divers D,Portier L,Turhan AG,Bennaceur Griscelli A

    更新日期:2017-10-01 00:00:00

  • One-step generation of murine embryonic stem cell-derived mesoderm progenitors and chondrocytes in a serum-free monolayer differentiation system.

    abstract::Cartilage defects have limited capacity for repair and are often replaced by fibrocartilage with inferior mechanical properties. To overcome the limitations of artificial joint replacement, high-throughput screens (HTS) could be developed to identify molecules that stimulate differentiation and/or proliferation of art...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2010.08.007

    authors: Waese EY,Stanford WL

    更新日期:2011-01-01 00:00:00

  • A co-culture model of the hippocampal neurogenic niche reveals differential effects of astrocytes, endothelial cells and pericytes on proliferation and differentiation of adult murine precursor cells.

    abstract::The niche concept of stem cell biology proposes a functional unit between the precursor cells and their local microenvironment, to which several cell types might contribute by cell-cell contacts, extracellular matrix, and humoral factors. We here established three co-culture models (with cell types separated by membra...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2015.09.010

    authors: Ehret F,Vogler S,Kempermann G

    更新日期:2015-11-01 00:00:00

  • Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene.

    abstract::The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurg...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.01.004

    authors: Miere C,Hewitson H,Devito L,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

    更新日期:2016-03-01 00:00:00

  • Generation of human embryonic stem cells from abnormal blastocyst diagnosed with albinism.

    abstract::Human embryonic stem cell (hESC) line chHES-478 was derived from abnormal blastocyst diagnosed with albinism after preimplantation genetic diagnosis (PGD) treatment. DNA sequencing analysis confirmed that chHES-478 cell line carried a compound heterozygous mutation, c.896G>A(p.Arg299His) and c.929_930insC(p.Pro310Glnf...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.11.004

    authors: Sun Y,Zhou X,Chen J,Du J,Lu G,Lin G,Ouyang Q

    更新日期:2016-11-01 00:00:00

  • The Sirt1 activator SRT3025 expands hematopoietic stem and progenitor cells and improves hematopoiesis in Fanconi anemia mice.

    abstract::Fanconi anemia is a genetic bone marrow failure syndrome. The current treatment options are suboptimal and do not prevent the eventual onset of aplastic anemia requiring bone marrow transplantation. We previously showed that resveratrol, an antioxidant and an activator of the protein deacetylase Sirt1, enhanced hemato...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2015.05.007

    authors: Zhang QS,Deater M,Schubert K,Marquez-Loza L,Pelz C,Sinclair DA,Grompe M

    更新日期:2015-07-01 00:00:00

  • Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene.

    abstract::Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of esta...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.05.003

    authors: Itoh M,Kawagoe S,Tamai K,Okano HJ,Nakagawa H

    更新日期:2016-07-01 00:00:00

  • Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system.

    abstract::Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2020.102069

    authors: Zhang C,Yu Y,Li F,Lan X,Wang L

    更新日期:2020-12-01 00:00:00

  • Generation of two human induced pluripotent stem cell lines, UNIBSi012-A and UNIBSi013-A, from two patients with treatment-resistant depression.

    abstract::Novel and complementary experimental models are required for investigating the molecular mechanisms underlying the resistance to the available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third of patients and need to be deeply investigated. Here, we hav...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2020.102104

    authors: Bono F,Mutti V,Piovani G,Minelli A,Mingardi J,Guglielmi A,Fiorentini C,Barbon A,Missale C,Gennarelli M

    更新日期:2020-12-01 00:00:00

  • Impact of transient down-regulation of DREAM in human embryonic stem cell pluripotency: The role of DREAM in the maintenance of hESCs.

    abstract::Little is known about the functions of downstream regulatory element antagonist modulator (DREAM) in embryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca(2+)-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are inv...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.03.001

    authors: Fontán-Lozano A,Capilla-Gonzalez V,Aguilera Y,Mellado N,Carrión AM,Soria B,Hmadcha A

    更新日期:2016-05-01 00:00:00

  • NANOG restores the impaired myogenic differentiation potential of skeletal myoblasts after multiple population doublings.

    abstract::Adult skeletal muscle regeneration relies on the activity of satellite cells residing in the skeletal muscle niche. However, systemic and intrinsic factors decrease the myogenic differentiation potential of satellite cells thereby impairing muscle regeneration. Here we present data showing that late passage C2C12 myob...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2017.11.018

    authors: Shahini A,Choudhury D,Asmani M,Zhao R,Lei P,Andreadis ST

    更新日期:2018-01-01 00:00:00

  • Transcriptional regulation of TRKC by SOX2 in human embryonic stem cells.

    abstract::Human embryonic stem (hES) cells have the dual ability to self-renew and differentiate into specialized cell types. However, in order to realize the full potential of these cells it is important to understand how the genes responsible for their unique characteristics are regulated. In this study we examine the regulat...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2011.10.003

    authors: Fong H,Wong RC,Donovan PJ

    更新日期:2012-03-01 00:00:00

  • Induced pluripotent stem cell line derived from a sporadic amyotrophic lateral sclerosis patient.

    abstract::Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 60-year-old female diagnosed with sporadic amyotrophic lateral sclerosis (sALS). The iPSCs shared the same karyotype with the parent PBMCs, expressed pluripotency stem cell markers, and demonstrated tr...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2020.101841

    authors: Feng B,Ma J,Amponsah AE,Guo R,Kong D,He J,Jiang Y,Zhang W,Zhang Z,Song Y,Shen S,O'Brien T,Cui H

    更新日期:2020-05-01 00:00:00

  • Generation of an iPSC line of a patient with Angelman syndrome due to an imprinting defect.

    abstract::Angelman syndrome (AS) is a neurodevelopmental disorder with leading symptoms of happy demeanor, intellectual disability, ataxia and seizures. AS can be caused by genetic and epigenetic aberrations, resulting in the absence of functional UBE3A protein in the brain. UBE3A is an imprinted gene, which is, in neurons of t...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2018.09.015

    authors: Neureiter A,Brändl B,Hiber M,Tandon R,Müller FJ,Steenpass L

    更新日期:2018-12-01 00:00:00

  • A multiplexed screening method for pluripotency.

    abstract::Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available ...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2017.07.014

    authors: Plotnikov A,Kozer N,Krupalnik V,Peles S,Mor N,Rais Y,Hanna JH,Barr HM

    更新日期:2017-08-01 00:00:00

  • Establishment of an induced pluripotent stem cell line (FDEENTi002-A) from a patient with Best's disease carrying c.888C > A mutation in BEST1 gene.

    abstract::Best's disease (BD) is an inherited retinal degenerative disease caused by mutations in BEST1 gene. A human induced pluripotent stem cell (iPSC) line has been generated with integration-free Sendai virus method from peripheral blood mononuclear cells (PBMCs) of a BD patient carrying c.888C > A mutation in BEST1 gene. ...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2019.101459

    authors: Bai X,Yang XJ,Chen L

    更新日期:2019-07-01 00:00:00

  • Development of FGF2-dependent pluripotent stem cells showing naive state characteristics from murine preimplantation inner cell mass.

    abstract::Two distinct types of embryonic pluripotent stem cells can be established from either the inner cell mass (ICM) of preimplantation blastocyst (leukemia inhibitory factor (LIF)-dependent embryonic stem cell, ESC, called naive state) or the epiblast of postimplantation fetuses (fibroblast growth factor 2 (FGF2)-dependen...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2014.04.012

    authors: Ozawa M,Kawakami E,Sakamoto R,Shibasaki T,Goto A,Yoshida N

    更新日期:2014-07-01 00:00:00

  • A novel variant of Oct3/4 gene in mouse embryonic stem cells.

    abstract::OCT4 is a highly conserved gene and plays an important role during early embryonic development and differentiation. Similar to human OCT4, mouse Oct4 gene generates variants. Oct4A is a master regulator of self-renewal in pluripotent stem cells. In this study, we have identified a novel Oct4 spliced variant, designate...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2012.04.004

    authors: Guo CL,Liu L,Jia YD,Zhao XY,Zhou Q,Wang L

    更新日期:2012-09-01 00:00:00

  • Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).

    abstract::The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated h...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2020.102126

    authors: Siehler J,Blöchinger AK,Akgün M,Wang X,Shahryari A,Geerlof A,Lickert H,Burtscher I

    更新日期:2020-12-16 00:00:00

  • Generation of KCL017 research grade human embryonic stem cell line carrying a mutation in VHL gene.

    abstract::The KCL017 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676+3A>T). The ICM was isolated using laser microsurgery and plated on γ...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.01.013

    authors: Hewitson H,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

    更新日期:2016-03-01 00:00:00

  • Generation of induced pluripotent stem cells (iPSCs) from a hereditary spastic paraplegia patient carrying a homozygous Y275X mutation in CYP7B1 (SPG5).

    abstract::Skin fibroblasts were obtained from a 47-year-old hereditary spastic paraplegia patient carrying a homozygous mutation Y275X in CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1), responsible for causing hereditary spastic paraplegia type 5 (SPG5). Induced pluripotent stem cells (iPSCs) were generated by t...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.09.011

    authors: Hauser S,Höflinger P,Theurer Y,Rattay TW,Schöls L

    更新日期:2016-09-01 00:00:00

  • Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible.

    abstract::Primary hepatocyte transplantation (HTx) is a safe cell therapy for patients with liver disease, but wider application is circumvented by poor cell engraftment due to limitations in hepatocyte quality and transplantation strategies. Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (hiPSC)...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2019.101599

    authors: Ashmore-Harris C,Blackford SJ,Grimsdell B,Kurtys E,Glatz MC,Rashid TS,Fruhwirth GO

    更新日期:2019-12-01 00:00:00

  • Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors.

    abstract::Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiati...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2017.03.010

    authors: Táncos Z,Nemes C,Varga E,Bock I,Rungarunlert S,Tharasanit T,Techakumphu M,Kobolák J,Dinnyés A

    更新日期:2017-05-01 00:00:00

  • Generation of a human iPS cell line (CGMH.SLC26A4919-2) from a Pendred syndrome patient carrying SLC26A4 c.919-2A>G splice-site mutation.

    abstract::SLC26A4 is the second most frequent gene implicated in congenital hearing loss after GJB2 mutations. Here, we report the generation of induced pluripotent stem cells (iPSCs), from a patient who was carrying a homozygous c.919-2A>G variant in the SLC26A4 gene. This is the most common variant of SLC26A4 gene in the Chin...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2019.101524

    authors: Cheng YF,Chan YH,Hu CJ,Lu YC,Saeki T,Hosoya M,Saegusa C,Fujioka M,Okano H,Weng SM,Hsu CJ,Chang KH,Wu CC

    更新日期:2019-10-01 00:00:00

  • Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain.

    abstract::Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed...

    journal_title:Stem cell research

    pub_type: 杂志文章

    doi:10.1016/j.scr.2016.04.015

    authors: Hallmann AL,Araúzo-Bravo MJ,Zerfass C,Senner V,Ehrlich M,Psathaki OE,Han DW,Tapia N,Zaehres H,Schöler HR,Kuhlmann T,Hargus G

    更新日期:2016-05-01 00:00:00