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The (not so) immortal strand hypothesis.

Abstract：

BACKGROUND:Non-random segregation of DNA strands during stem cell replication has been proposed as a mechanism to minimize accumulated genetic errors in stem cells of rapidly dividing tissues. According to this hypothesis, an "immortal" DNA strand is passed to the stem cell daughter and not the more differentiated cell, keeping the stem cell lineage replication error-free. After it was introduced, experimental evidence both in favor and against the hypothesis has been presented. PRINCIPAL FINDINGS:Using a novel methodology that utilizes cancer sequencing data we are able to estimate the rate of accumulation of mutations in healthy stem cells of the colon, blood and head and neck tissues. We find that in these tissues mutations in stem cells accumulate at rates strikingly similar to those expected without the protection from the immortal strand mechanism. SIGNIFICANCE:Utilizing an approach that is fundamentally different from previous efforts to confirm or refute the immortal strand hypothesis, we provide evidence against non-random segregation of DNA during stem cell replication. Our results strongly suggest that parental DNA is passed randomly to stem cell daughters and provides new insight into the mechanism of DNA replication in stem cells.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Tomasetti C,Bozic I

doi

10.1016/j.scr.2015.01.005

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

238-41

issue

2

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(15)00014-8

journal_volume

14

pub_type

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