Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain.

Abstract:

:Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed transcriptome analyses on murine iNSCs in comparison to brain-derived neural stem cells (NSCs) and pluripotent stem cell-derived NPCs, which revealed distinct global, neural, metabolic and cell cycle-associated marks in these populations. iNSCs carried a hindbrain/posterior cell identity, which could be shifted towards caudal, partially to rostral but not towards ventral fates in vitro. iNSCs survived after transplantation into the rodent brain and exhibited in vivo-characteristics, neural and metabolic programs similar to transplanted NSCs. However, iNSCs vastly retained caudal identities demonstrating cell-autonomy of regional programs in vivo. These data could have significant implications for a variety of in vitro- and in vivo-applications using iNSCs.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Hallmann AL,Araúzo-Bravo MJ,Zerfass C,Senner V,Ehrlich M,Psathaki OE,Han DW,Tapia N,Zaehres H,Schöler HR,Kuhlmann T,Hargus G

doi

10.1016/j.scr.2016.04.015

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

776-81

issue

3

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(16)30026-5

journal_volume

16

pub_type

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