Abstract:
:Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, RDEB-iPSC26, was confirmed by the expressions of stem cell markers and the differentiation capability into three germ layer. RDEB-iPSC26 may be a useful cell resource for the establishment of in vitro RDEB modeling and the study for developing gene and cell therapy.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Itoh M,Kawagoe S,Tamai K,Okano HJ,Nakagawa Hdoi
10.1016/j.scr.2016.05.003subject
Has Abstractpub_date
2016-07-01 00:00:00pages
32-35issue
1eissn
1873-5061issn
1876-7753pii
S1873-5061(16)30034-4journal_volume
17pub_type
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