Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene.

Abstract:

:Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, RDEB-iPSC26, was confirmed by the expressions of stem cell markers and the differentiation capability into three germ layer. RDEB-iPSC26 may be a useful cell resource for the establishment of in vitro RDEB modeling and the study for developing gene and cell therapy.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Itoh M,Kawagoe S,Tamai K,Okano HJ,Nakagawa H

doi

10.1016/j.scr.2016.05.003

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

32-35

issue

1

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(16)30034-4

journal_volume

17

pub_type

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