Abstract:
:Gene therapy mediated by bone marrow-derived hematopoietic stem cells (BM-HSC) has been widely used in treating genetic deficiencies in both pre-clinical and clinical settings. Using mitotically inactive cell-targeting lentivirus with separate promoters for our gene of interest (the murine MHC class II (MHCII) chaperone, invariant chain (Ii)) and a GFP reporter, we monitored the expression and function of introduced Ii in various types of professional antigen presenting cells (B cells, macrophages and DC) from different organs (spleen, pancreatic lymph nodes (PLN), BM and blood). Ii and GFP were detected. Ii levels correlated with GFP levels only in macrophages and monocytes from spleen, monocytes from PLN and macrophage precursors from blood. By cell type, Ii levels in PLN cells were more similar to those in spleen cells than to those in blood or BM cells. Functionally, Ii expressed in PLN or spleen had more effect on MHCII abundance than Ii expressed in BM or blood. The results have implications for analysis of the outcomes of gene therapy when both therapeutic and reporter genes are introduced. The findings also have implications for understanding the development of immune molecule function.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Wang N,Rajasekaran N,Hou T,Mellins EDdoi
10.1016/j.scr.2013.10.010subject
Has Abstractpub_date
2014-01-01 00:00:00pages
209-21issue
1eissn
1873-5061issn
1876-7753pii
S1873-5061(13)00160-8journal_volume
12pub_type
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.05.006
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.10.001
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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abstract::Skin fibroblasts were collected from a 65-year-old male patient with sporadic Parkinson's disease. Induced pluripotent stem cells were reprogrammed with human reprogramming factors (KMOSL) using the messenger RNA reprogramming protocol. The transgene-free iPSC line showed pluripotency, displayed normal karyotype, and ...
journal_title:Stem cell research
pub_type: 杂志文章
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