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Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing.

Abstract：

:As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated with liver cancer development. Here, we demonstrate the generation of a heterozygous p53(R249S) mutation in the H9 human embryonic stem cell line using TALEN-mediated genome editing. The generated cell line maintains a normal karyotype, a pluripotent state and the in vivo capacity to develop a teratoma containing all three germ layer tissues.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Huo Z,Tu J,Xu A,Li Y,Wang D,Liu M,Zhou R,Zhu D,Lin Y,Gingold JA,Yen CJ,Xiao H,Zhao R

doi

10.1016/j.scr.2018.101360

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

101360

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(18)30290-3

journal_volume

34

pub_type

杂志文章

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