当前位置： SCI文献检索 > Stem Cell Research期刊下所有文献 > The cytokine-mediated crosstalk between primary human acute myeloid cells and mesenchymal stem cells alters the local cytokine network and the global gene expression profile of the mesenchymal cells.

The cytokine-mediated crosstalk between primary human acute myeloid cells and mesenchymal stem cells alters the local cytokine network and the global gene expression profile of the mesenchymal cells.

Abstract：

:Interactions between acute myeloid leukemia (AML) blasts and neighboring stromal cells are important for disease development and chemosensitivity. However, the molecular mechanisms involved in the cytokine-mediated crosstalk between mesenchymal stem cells (MSCs) and AML cells are largely unknown. Leukemic cells derived from 18 unselected AML patients were cultured with bone marrow MSCs derived from healthy donors; the populations then being separated by a semipermeable membrane. Coculture had only minor effects on MSC proliferation. The unique cytokine network in cocultures was determined by high constitutive MSC release of certain cytokines (especially IL-6 and vascular endothelial growth factor) and constitutive release of a wide range of soluble mediators by primary AML cells. However, the AML cell release varied considerably between patients, and these differences between patients were also reflected in the coculture levels even though supra-additive effects were seen for many mediators. These effects on the local cytokine network were dependent on a functional crosstalk between the two cell subsets. The crosstalk altered the global gene expression profile of the MSCs, especially expression of genes encoding proteins involved in downstream signaling from Toll like receptors, NFκB signaling and CCL/CXCL chemokine release. Thus, primary AML cells alter the functional phenotype of normal MSCs.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Reikvam H,Brenner AK,Hagen KM,Liseth K,Skrede S,Hatfield KJ,Bruserud Ø

doi

10.1016/j.scr.2015.09.008

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

530-541

issue

3

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(15)00121-X

journal_volume

15

pub_type

杂志文章

相关文献

Stem Cell Research文献大全
  • Excision of the expanded GAA repeats corrects cardiomyopathy phenotypes of iPSC-derived Friedreich's ataxia cardiomyocytes.

    abstract：:Friedreich's ataxia is caused by large homozygous, intronic expansions of GAA repeats in the frataxin (FXN) gene, resulting in severe downregulation of its expression. Pathogenic repeats are located in intron one, hence patients express unaffected FXN protein, albeit in low quantities. Although FRDA symptoms typically...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101529

    authors： Li J,Rozwadowska N,Clark A,Fil D,Napierala JS,Napierala M

    更新日期：2019-10-01 00:00:00

  • Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+T cells of healthy individuals of Indian origin.

    abstract：:Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressin...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101506

    authors： Khan M,Zende S,Vaidyanath A,Avatade R,Shiras A

    更新日期：2019-08-01 00:00:00

  • Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9.

    abstract：:FOS is component of the AP-1 complex and has been reported to be involved in many cellular functions, including cell proliferation, differentiation, survival, angiogenesis, hematopoiesis and cancer progress. To further understand the exact role of FOS in these processes, here we created two FOS knockout human embryoni...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101479

    authors： Li C,Wang Q,Peng Z,Lin Y,Liu H,Yang X,Li S,Liu X,Chen J

    更新日期：2019-08-01 00:00:00

  • Generation of 2 induced pluripotent stem cell lines derived from patients with Parkinson's disease carrying LRRK2 G2385R variant.

    abstract：:Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.01.034

    authors： Cheng YC,Huang CY,Ho MC,Hsu YH,Syu SH,Lu HE,Lin HI,Lin CH,Hsieh PCH

    更新日期：2018-04-01 00:00:00

  • uPAR and cathepsin B-mediated compartmentalization of JNK regulates the migration of glioma-initiating cells.

    abstract：:In the present study, we investigated the effect of simultaneous downregulation of uPAR and cathepsin B (pUC), alone or in combination with radiation, on JNK-MAPK signaling pathway in regulating the migration of non-GICs (glioma-initiating cells) and GICs. The increase in the expression of p-JNK with pUC treatment was...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2014.02.008

    authors： Alapati K,Kesanakurti D,Rao JS,Dasari VR

    更新日期：2014-05-01 00:00:00

  • Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system.

    abstract：:Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Amniotic fluid (AF) cells are easy to obtain from routinely scheduled procedures for prenatal diagnosis and iPS cells have been generated from human AF. H...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.09.009

    authors： Bertin E,Piccoli M,Franzin C,Nagy A,Mileikovsky M,De Coppi P,Pozzobon M

    更新日期：2015-11-01 00:00:00

  • Adipose-derived stromal cell secretome reduces TNFα-induced hypertrophy and catabolic markers in primary human articular chondrocytes.

    abstract：:Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting the osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent a valid therapeutic substitute. The aim of this study was to investigate the e...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101463

    authors： Niada S,Giannasi C,Gomarasca M,Stanco D,Casati S,Brini AT

    更新日期：2019-07-01 00:00:00

  • Derivation of NEM2 affected human embryonic stem cell line Genea079.

    abstract：:The Genea079 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying compound heterozygous mutations in the NEB gene, exon 55 deletion & c.15110dupA, indicative of Nemaline Myopathy Type 2 (NEM2). Following ICM outgrowth on inactivated human feeders, karyotype w...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.02.015

    authors： Dumevska B,McKernan R,Goel D,Schmidt U

    更新日期：2016-03-01 00:00:00

  • L-WRN conditioned medium for gastrointestinal epithelial stem cell culture shows replicable batch-to-batch activity levels across multiple research teams.

    abstract：:Conditioned medium (CM) derived from engineered cells often facilitates the cost-effective culture of a variety of stem cells. Growing emphasis on the importance of rigor and reproducibility in lab-based science requires development of best practices approaches, including quality control procedures for the assessment ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101430

    authors： VanDussen KL,Sonnek NM,Stappenbeck TS

    更新日期：2019-05-01 00:00:00

  • Mesenchymal stromal cells up-regulate CD39 and increase adenosine production to suppress activated T-lymphocytes.

    abstract：:Mesenchymal stromal cells (MSCs) suppress T cell responses through mechanisms not completely understood. Adenosine is a strong immunosuppressant that acts mainly through its receptor A(2a) (ADORA2A). Extracellular adenosine levels are a net result of its production (mediated by CD39 and CD73), and of its conversion in...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2011.04.001

    authors： Saldanha-Araujo F,Ferreira FI,Palma PV,Araujo AG,Queiroz RH,Covas DT,Zago MA,Panepucci RA

    更新日期：2011-07-01 00:00:00

  • Optimized procedures for generating an enhanced, near physiological 2D culture system from porcine intestinal organoids.

    abstract：:An important practical limitation of the three-dimensional geometry of stem-cell derived intestinal organoids is that it prevents easy access to the apical epithelium for testing food components, microorganisms, bioactive and toxic compounds. To this end, we here report on a new robust method for generating confluent ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.02.013

    authors： van der Hee B,Loonen LMP,Taverne N,Taverne-Thiele JJ,Smidt H,Wells JM

    更新日期：2018-04-01 00:00:00

  • Promotion of hematopoietic differentiation from mouse induced pluripotent stem cells by transient HoxB4 transduction.

    abstract：:Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2011.09.001

    authors： Tashiro K,Kawabata K,Omori M,Yamaguchi T,Sakurai F,Katayama K,Hayakawa T,Mizuguchi H

    更新日期：2012-03-01 00:00:00

  • Generation of an induced pluripotent stem cell line (SHCDNRi001-A) from a patient with X-linked Alport syndrome carrying a heterozygous p.G409S (c. 1225 G > A) mutation in the COL4A5 gene.

    abstract：:X-linked Alport syndrome (XLAS) is a rare form of hereditary nephritis caused by mutations in the COL4A5 gene encoding the type IV collagen α5 chain. A skin biopsy was performed on one female patient with XLAS who carried a heterozygous p.G409S (c. 1225 G > A) mutation in the COL4A5 gene. A human-induced pluripotent s...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101833

    authors： Sun L,Zhang J,Kuang XY,Kang YL,Wu Y,Huang WY

    更新日期：2020-05-01 00:00:00

  • Eltrombopag, a thrombopoietin receptor agonist, enhances human umbilical cord blood hematopoietic stem/primitive progenitor cell expansion and promotes multi-lineage hematopoiesis.

    abstract：:Umbilical cord blood (UCB) transplantation has emerged as a promising therapy, but it is challenged by scarcity of stem cells. Eltrombopag is a non-peptide, thrombopoietin (TPO) receptor agonist, which selectively activates c-Mpl in humans and chimpanzees. We investigated eltrombopag's effects on human UCB hematopoiet...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2012.05.001

    authors： Sun H,Tsai Y,Nowak I,Liesveld J,Chen Y

    更新日期：2012-09-01 00:00:00

  • Generation of a human control iPSC line with a European mitochondrial haplogroup U background.

    abstract：:Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus. ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.12.010

    authors： Galera T,Zurita F,González-Páramos C,Moreno-Izquierdo A,Fraga MF,Fernández AF,Garesse R,Gallardo ME

    更新日期：2016-01-01 00:00:00

  • Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene.

    abstract：:Incomplete achromatopsia (ACHM) is a disorder in which there is function defect of cone photoreceptors in the retina and individuals with such disease retain residual color vision. Here, we have generated an induced pluripotent stem cell (iPSC) line carrying a homozygous mutation in the PDE6C gene, already related wit...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101569

    authors： Domingo-Prim J,Abad-Morales V,Riera M,Navarro R,Corcostegui B,Pomares E

    更新日期：2019-10-01 00:00:00

  • Establishment of induced pluripotent stem cell line (GSPHi001-A) from a 6-year old female with nephrotic syndrome.

    abstract：:Peripheral blood mononuclear cells (PBMCs) were collected from a 6-year-old female child who was clinically diagnosed as primary nephrotic syndrome (NS) with hormone resistance. An iPSC line was successfully established by the Sendai-virus (SeV) delivery system. The iPS-19 (GSPHi001-A) expressed pluripotent markers, e...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101661

    authors： Gao X,Gao X,Zhao H,Cui W,Tan M,Deng H

    更新日期：2019-12-01 00:00:00

  • The Sirt1 activator SRT3025 expands hematopoietic stem and progenitor cells and improves hematopoiesis in Fanconi anemia mice.

    abstract：:Fanconi anemia is a genetic bone marrow failure syndrome. The current treatment options are suboptimal and do not prevent the eventual onset of aplastic anemia requiring bone marrow transplantation. We previously showed that resveratrol, an antioxidant and an activator of the protein deacetylase Sirt1, enhanced hemato...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.05.007

    authors： Zhang QS,Deater M,Schubert K,Marquez-Loza L,Pelz C,Sinclair DA,Grompe M

    更新日期：2015-07-01 00:00:00

  • Generation of non-integrated induced pluripotent stem cells from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome.

    abstract：:Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was con...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.12.002

    authors： Guo D,Wu F,Liu H,Gao G,Kou S,Yang F,Abbas N,Zhou T,Cai X,Zhang H,Qin D,Li J,Xu K,Li YX

    更新日期：2017-01-01 00:00:00

  • Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease.

    abstract：:Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.12.026

    authors： Chumarina M,Azevedo C,Bigarreau J,Vignon C,Kim KS,Li JY,Roybon L

    更新日期：2017-03-01 00:00:00

  • An integration-free iPSC line SDQLCHi025-A from a girl with multiminicore disease carrying compound heterozygote mutations in RYR1 gene.

    abstract：:Peripheral blood mononuclear cells for reprogramming in this work were donated by a girl with clinically and genetically diagnosed multiminicore disease harboring compound heterozygote mutations of RYR1 gene. Induced pluripotent stem cells (iPSCs) were obtained by non-integrating episomal vectors containing OCT4, SOX2...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101775

    authors： Zhang H,Ma Y,Lv Y,Wan Y,Zhao Q,Gai Z,Liu Y

    更新日期：2020-05-01 00:00:00

  • Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).

    abstract：:The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated h...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102126

    authors： Siehler J,Blöchinger AK,Akgün M,Wang X,Shahryari A,Geerlof A,Lickert H,Burtscher I

    更新日期：2020-12-16 00:00:00

  • Generation of two human induced pluripotent stem cell (hiPSC) lines from a long QT syndrome South African founder population.

    abstract：:We generated PSMi001-A and PSMi008-A hiPSC lines from two individuals belonging to a South African (SA) founder population in which the malignant KCNQ1-A341V mutation cosegregates with the Long QT Syndrome (LQTS) phenotype. PSMi001-A was derived from an asymptomatic KCNQ1-A341V mutation carrier, whereas PSMi008-A was ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101510

    authors： Mura M,Pisano F,Stefanello M,Ginevrino M,Boni M,Calabrò F,Crotti L,Valente EM,Schwartz PJ,Brink PA,Gnecchi M

    更新日期：2019-08-01 00:00:00

  • Generation of the human induced pluripotent stem cell line (SHAMUi001-A) carrying the heterozygous c.-128G>T mutation in the 5'-UTR of the ANKRD26 gene.

    abstract：:Thrombocytopenia 2 (THC2) is a major type of inherited thrombocytopenia caused by the persistent ANKRD26 expression during the late stage of megakaryocytopoiesis. For the first time, we generated a human induced pluripotent stem cell (hiPSC) line SHAMUi001-A from the bone marrow hematopoietic progenitor cells of a THC...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102002

    authors： Tan C,Dai L,Yang W,Li F,Wang L,Xiao Y,Wang X,Zhang Y,Wang Y,Zeng C,Xiang Z,Zhang X,Zhang W,Ran Q,Chen M,Li Z,Chen L

    更新日期：2020-10-01 00:00:00

  • Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro.

    abstract：:Optimization of pluripotent stem cell expansion and differentiation is facilitated by biological tools that permit non-invasive and dynamic monitoring of pluripotency, and the ability to select for an undifferentiated input cell population. Here we report on the generation and characterisation of clonal human embryoni...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2014.05.006

    authors： Ovchinnikov DA,Titmarsh DM,Fortuna PR,Hidalgo A,Alharbi S,Whitworth DJ,Cooper-White JJ,Wolvetang EJ

    更新日期：2014-09-01 00:00:00

  • Generation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering Williams-Beuren syndrome (7q11.23 deletion).

    abstract：:Skin fibroblasts were obtained from four patients with Williams-Beuren syndrome (WBS) carrying the typical 1.5 Mb or 1.8 Mb deletion at the 7q11.23 genomic region. Induced pluripotent stem cells (iPSCs) were generated by retroviral infection of fibroblasts with polycystronic vectors. The generated iPSC clones ESi059A,...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102087

    authors： Kuebler B,Aran B,Flores R,Pérez-Jurado LA,Veiga A,Corominas R,Cuscó I

    更新日期：2020-12-01 00:00:00

  • Derivation of a disease-specific human induced pluripotent stem cell line from a biliary atresia patient.

    abstract：:Biliary atresia (BA) is a common cause of pediatric end-stage liver disease. While its etiology is not yet clear, evidence has suggested that BA results from interactions between genetic susceptibility and environmental factors. Disease relevant human cellular models of BA will facilitate identification of both geneti...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2017.08.001

    authors： Tian L,Eldridge L,Chaudhari P,Zhang L,Anders RA,Schwarz KB,Ye Z,Jang YY

    更新日期：2017-10-01 00:00:00

  • Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors.

    abstract：:Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiati...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2017.03.010

    authors： Táncos Z,Nemes C,Varga E,Bock I,Rungarunlert S,Tharasanit T,Techakumphu M,Kobolák J,Dinnyés A

    更新日期：2017-05-01 00:00:00

  • Derivation of the human embryonic stem cell line RCe011-A (RC-7).

    abstract：:The human embryonic stem cell line RCe011-A (RC-7) was derived from a failed to fertilise oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows norma...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.02.036

    authors： De Sousa PA,Tye BJ,Collins DM,Bruce K,Dand P,Russell G,Bradburn H,Downie JM,Bateman M,Courtney A

    更新日期：2016-03-01 00:00:00

  • Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes.

    abstract：:We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) w...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.07.007

    authors： Fatima A,Xu G,Nguemo F,Kuzmenkin A,Burkert K,Hescheler J,Šarić T

    更新日期：2016-09-01 00:00:00