Abstract:
:Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES cells. However, these methods cannot be applied therapeutically due to the risk of transgenes being integrated into the host genome. Here, we report the promotion of hematopoietic differentiation from mouse ES cells and induced pluripotent stem (iPS) cells by transient HoxB4 expression using an adenovirus (Ad) vector. Ad vector could mediate efficient HoxB4 expression in ES cell-derived embryoid bodies (ES-EBs) and iPS-EBs, and its expression was decreased during cultivation, showing that Ad vector transduction was transient. A colony-forming assay revealed that the number of hematopoietic progenitor cells with colony-forming potential in HoxB4-transduced cells was significantly increased in comparison with that in non-transduced cells or LacZ-transduced cells. HoxB4-transduced cells also showed more efficient generation of CD41-, CD45-, or Sca-1-positive cells than control cells. These results indicate that transient, but not constitutive, HoxB4 expression is sufficient to augment the hematopoietic differentiation of ES and iPS cells, and that our method would be useful for clinical applications, such as cell transplantation therapy.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Tashiro K,Kawabata K,Omori M,Yamaguchi T,Sakurai F,Katayama K,Hayakawa T,Mizuguchi Hdoi
10.1016/j.scr.2011.09.001subject
Has Abstractpub_date
2012-03-01 00:00:00pages
300-11issue
2eissn
1873-5061issn
1876-7753pii
S1873-5061(11)00115-2journal_volume
8pub_type
杂志文章abstract::Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 60-year-old female diagnosed with sporadic amyotrophic lateral sclerosis (sALS). The iPSCs shared the same karyotype with the parent PBMCs, expressed pluripotency stem cell markers, and demonstrated tr...
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2010.08.002
更新日期:2011-01-01 00:00:00
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journal_title:Stem cell research
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pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
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journal_title:Stem cell research
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