Abstract:
:FOS is component of the AP-1 complex and has been reported to be involved in many cellular functions, including cell proliferation, differentiation, survival, angiogenesis, hematopoiesis and cancer progress. To further understand the exact role of FOS in these processes, here we created two FOS knockout human embryonic stem cell lines by CRISPR/Cas9 mediated gene targeting. These cell lines retained normal morphology and karyotype, normal expression of pluripotent markers, and differentiation potential both in vivo and in vitro. These cell lines can be used to verify whether the FOS mutated produces any affect on endothelial cells and hematopoietic progenitor cells during the hematopoietic differentiation.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Li C,Wang Q,Peng Z,Lin Y,Liu H,Yang X,Li S,Liu X,Chen Jdoi
10.1016/j.scr.2019.101479subject
Has Abstractpub_date
2019-08-01 00:00:00pages
101479eissn
1873-5061issn
1876-7753pii
S1873-5061(19)30109-6journal_volume
39pub_type
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