Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).

Abstract:

:The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Siehler J,Blöchinger AK,Akgün M,Wang X,Shahryari A,Geerlof A,Lickert H,Burtscher I

doi

10.1016/j.scr.2020.102126

subject

Has Abstract

pub_date

2020-12-16 00:00:00

pages

102126

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(20)30427-X

journal_volume

50

pub_type

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