Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).


:The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.


Stem Cell Res


Stem cell research


Siehler J,Blöchinger AK,Akgün M,Wang X,Shahryari A,Geerlof A,Lickert H,Burtscher I




Has Abstract


2020-12-16 00:00:00












  • Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla.

    abstract::Insulin-like growth factor 1 (IGF-1) plays an important role in the regulation of tooth root development, and stem cells from apical papilla (SCAPs) are responsible for the formation of root pulp and dentin. To date, it remains unclear whether IGF-1 can regulate the function of SCAPs. In this study, SCAPs were isolate...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Wang S,Mu J,Fan Z,Yu Y,Yan M,Lei G,Tang C,Wang Z,Zheng Y,Yu J,Zhang G

    更新日期:2012-05-01 00:00:00

  • Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults.

    abstract::We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Vlahos K,Sourris K,Mayberry R,McDonald P,Bruveris FF,Schiesser JV,Bozaoglu K,Lockhart PJ,Stanley EG,Elefanty AG

    更新日期:2019-01-01 00:00:00

  • Generation of an induced pluripotent stem cell line (FRIMOi007-A) derived from an incomplete achromatopsia patient carrying a novel homozygous mutation in PDE6C gene.

    abstract::Incomplete achromatopsia (ACHM) is a disorder in which there is function defect of cone photoreceptors in the retina and individuals with such disease retain residual color vision. Here, we have generated an induced pluripotent stem cell (iPSC) line carrying a homozygous mutation in the PDE6C gene, already related wit...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Domingo-Prim J,Abad-Morales V,Riera M,Navarro R,Corcostegui B,Pomares E

    更新日期:2019-10-01 00:00:00

  • L-WRN conditioned medium for gastrointestinal epithelial stem cell culture shows replicable batch-to-batch activity levels across multiple research teams.

    abstract::Conditioned medium (CM) derived from engineered cells often facilitates the cost-effective culture of a variety of stem cells. Growing emphasis on the importance of rigor and reproducibility in lab-based science requires development of best practices approaches, including quality control procedures for the assessment ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: VanDussen KL,Sonnek NM,Stappenbeck TS

    更新日期:2019-05-01 00:00:00

  • Stem cell passage affects directional migration of stem cells in electrotaxis.

    abstract::Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. He...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Hong SH,Lee MH,Koo MA,Seon GM,Park YJ,Kim D,Park JC

    更新日期:2019-07-01 00:00:00

  • A multiplexed screening method for pluripotency.

    abstract::Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Plotnikov A,Kozer N,Krupalnik V,Peles S,Mor N,Rais Y,Hanna JH,Barr HM

    更新日期:2017-08-01 00:00:00

  • A novel variant of Oct3/4 gene in mouse embryonic stem cells.

    abstract::OCT4 is a highly conserved gene and plays an important role during early embryonic development and differentiation. Similar to human OCT4, mouse Oct4 gene generates variants. Oct4A is a master regulator of self-renewal in pluripotent stem cells. In this study, we have identified a novel Oct4 spliced variant, designate...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Guo CL,Liu L,Jia YD,Zhao XY,Zhou Q,Wang L

    更新日期:2012-09-01 00:00:00

  • Generation of induced pluripotent stem cells-derived hepatocyte-like cells for ex vivo gene therapy of primary hyperoxaluria type 1.

    abstract::Primary hyperoxaluria type 1 (PH1) is a rare autosomal recessive disorder of the liver metabolism due to functional deficiency of the peroxisomal enzyme alanine:glyoxylate aminotransferase (AGT). AGT deficiency results in overproduction of oxalate which complexes with calcium to form insoluble calcium-oxalate salts in...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Estève J,Blouin JM,Lalanne M,Azzi-Martin L,Dubus P,Bidet A,Harambat J,Llanas B,Moranvillier I,Bedel A,Moreau-Gaudry F,Richard E

    更新日期:2019-07-01 00:00:00

  • Promotion of hematopoietic differentiation from mouse induced pluripotent stem cells by transient HoxB4 transduction.

    abstract::Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Tashiro K,Kawabata K,Omori M,Yamaguchi T,Sakurai F,Katayama K,Hayakawa T,Mizuguchi H

    更新日期:2012-03-01 00:00:00

  • Generation of an induced pluripotent stem cell line, CSSi011-A (6534), from an Amyotrophic lateral sclerosis patient with heterozygous L145F mutation in SOD1 gene.

    abstract::Among the known causative genes of familial ALS, SOD1mutation is one of the most common. It encodes for the ubiquitous detoxifying copper/zinc binding SOD1 enzyme, whose mutations selectively cause motor neuron death, although the mechanisms are not as yet clear. What is known is that mutant-mediated toxicity is not c...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: D'Anzi A,Altieri F,Perciballi E,Ferrari D,Bernardini L,Goldoni M,Mazzini L,De Marchi F,Di Pierro A,D'Alfonso S,Gelati M,Vescovi AL,Rosati J

    更新日期:2020-07-25 00:00:00

  • Derivation of NEM2 affected human embryonic stem cell line Genea079.

    abstract::The Genea079 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying compound heterozygous mutations in the NEB gene, exon 55 deletion & c.15110dupA, indicative of Nemaline Myopathy Type 2 (NEM2). Following ICM outgrowth on inactivated human feeders, karyotype w...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Dumevska B,McKernan R,Goel D,Schmidt U

    更新日期:2016-03-01 00:00:00

  • Neuro-peptide treatment with Cerebrolysin improves the survival of neural stem cell grafts in an APP transgenic model of Alzheimer disease.

    abstract::Neural stem cells (NSCs) have been considered as potential therapy in Alzheimer's disease (AD) but their use is hampered by the poor survival of grafted cells. Supply of neurotrophic factors to the grafted cells has been proposed as a way to augment survival of the stem cells. In this context, we investigated the util...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Rockenstein E,Desplats P,Ubhi K,Mante M,Florio J,Adame A,Winter S,Brandstaetter H,Meier D,Masliah E

    更新日期:2015-07-01 00:00:00

  • Detection of mouse endogenous type B astrocytes migrating towards brain lesions.

    abstract::Neuroblasts represent the predominant migrating cell type in the adult mouse brain. There are, however, increasing evidences of migration of other neural precursors. This work aims at identifying in vivo endogenous early neural precursors, different from neuroblasts, able to migrate in response to brain injuries. The ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Elvira G,García I,Gallo J,Benito M,Montesinos P,Holgado-Martin E,Ayuso-Sacido A,Penadés S,Desco M,Silva A,Garcia-Sanz JA

    更新日期:2015-01-01 00:00:00

  • Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD).

    abstract::An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency marker...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Wang Y,Zhang J,Lei Y,Zhao J

    更新日期:2018-12-01 00:00:00

  • Generation of induced pluripotent stem cell line (ZZUi007-A) from a 52-year-old patient with a novel CHCHD2 gene mutation in Parkinson's disease.

    abstract::CHCHD2 mutation has been reported as a potential cause of a rare form of familial Parkinson's disease. Recently, a novel CHCHD2 mutation was identified in a family with Parkinson's disease. The dermal fibroblasts of the patient were obtained and successfully transformed into induced pluripotent stem cells(iPSCs), empl...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Wang Y,Wang Z,Sun H,Mao C,Yang J,Liu Y,Liu H,Zhang S,Zhang J,Xu Y,Shi C

    更新日期:2018-10-01 00:00:00

  • An induced pluripotent stem cell line (TRNDi009-C) from a Niemann-Pick disease type A patient carrying a heterozygous p.L302P (c.905 T>C) mutation in the SMPD1 gene.

    abstract::Niemann-Pick disease type A (NPA) is a rare autosomal recessive lysosomal storage disease caused by mutations in the SMPD1 gene, which encodes for the protein acid sphingomyelinase. A human induced pluripotent stem cell (iPSC) line was generated from dermal fibroblasts of a 21-fetal-week-old female patient with NPA th...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Baskfield A,Li R,Beers J,Zou J,Liu C,Zheng W

    更新日期:2019-07-01 00:00:00

  • Inhibition of focal adhesion kinase increases adult olfactory stem cell self-renewal and neuroregeneration through ciliary neurotrophic factor.

    abstract::Constant neuroregeneration in adult olfactory epithelium maintains olfactory function by basal stem cell proliferation and differentiation to replace lost olfactory sensory neurons (OSNs). Understanding the mechanisms regulating this process could reveal potential therapeutic targets for stimulating adult olfactory ne...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Jia C,Oliver J,Gilmer D,Lovins C,Rodriguez-Gil DJ,Hagg T

    更新日期:2020-12-01 00:00:00

  • Generation of two induced pluripotent stem cell lines from a patient with Stargardt Macular Dystrophy caused by the c.768G>T and c.6079C>T mutations in ABCA4.

    abstract::Autosomal recessive Stargardt disease is the most common cause of inherited retinal disease. In this report, we describe the generation and characterization of two human induced pluripotent stem cell (iPSC) lines from a patient with compound heterozygous mutations in the ABCA4 gene (c.[768G>T];[6079C>T]). Patient derm...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Jennings L,Zhang D,Chen SC,Moon SY,Lamey T,Thompson JA,McLaren T,De Roach JN,Chen FK,McLenachan S

    更新日期:2020-10-01 00:00:00

  • Generation of KCL035 research grade human embryonic stem cell line carrying a mutation in HBB gene.

    abstract::The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propag...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Hewitson H,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

    更新日期:2016-03-01 00:00:00

  • Integration-free induced pluripotent stem cells derived from a patient with autosomal recessive Alport syndrome (ARAS).

    abstract::A skin biopsy was obtained from a 25-year-old female patient with autosomal recessive Alport syndrome (ARAS) with the homozygous COL4A3 mutation c.345delG, p.(P166Lfs*37). Dermal fibroblasts were derived and reprogrammed by nucleofection with episomal plasmids carrying OCT3/4, SOX2, KLF4 LIN28, L-MYC and p53shRNA. The...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Kuebler B,Aran B,Miquel-Serra L,Muñoz Y,Ars E,Bullich G,Furlano M,Torra R,Marti M,Veiga A,Raya A

    更新日期:2017-12-01 00:00:00

  • Generation of induced pluripotent stem cell line (XDCMHi001-A) from an Ankylosing spondylitis patient with JAK2 mutation.

    abstract::Heredity is the major factor contributing to the susceptibility to ankylosing spondylitis(AS). Janus kinase 2 (JAK2) has been associated with AS. Urine-derived cells from an AS patient with JAK2 mutation were used to generate induced pluripotent stem cells (iPSCs) with five episomal iPSC reprogramming vectors (pCXLE-h...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Hu J,Ren W,Qiu W,Lv J,Zhang C,Xu C,DU W,Wu M,Wang J,Quan R

    更新日期:2020-05-01 00:00:00

  • Derivation and molecular characterization of pancreatic differentiated MODY1-iPSCs.

    abstract::Maturity onset diabetes of the young (MODY) is a hereditary form of diabetes mellitus presenting at childhood or adolescence, which eventually leads to pancreatic β-cells dysfunction. The underlying genetic basis of MODY disorders is haploinsufficiency, where loss-of-function mutations in a single allele cause the dia...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Braverman-Gross C,Nudel N,Ronen D,Beer NL,McCarthy MI,Benvenisty N

    更新日期:2018-08-01 00:00:00

  • Generation of an iPSC line of a patient with Angelman syndrome due to an imprinting defect.

    abstract::Angelman syndrome (AS) is a neurodevelopmental disorder with leading symptoms of happy demeanor, intellectual disability, ataxia and seizures. AS can be caused by genetic and epigenetic aberrations, resulting in the absence of functional UBE3A protein in the brain. UBE3A is an imprinted gene, which is, in neurons of t...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Neureiter A,Brändl B,Hiber M,Tandon R,Müller FJ,Steenpass L

    更新日期:2018-12-01 00:00:00

  • Establishment of induced pluripotent stem cell line CSUASOi004-A by reprogramming peripheral blood mononuclear cells of a PRPF6-related dominant retinitis pigmentosa patient.

    abstract::We have established the patient-specific induced pluripotent stem (iPS) cell line CSUASOi004-A by using peripheral blood mononuclear cells (PBMCs) of a retinitis pigmentosa (RP) patient with a PRPF6 gene mutation (c.G2699A:p.R900H). CSUASOi004-A was established by a non-integrative method with four episomal plasmids c...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Zhou Y,Jing Y,Mao S,Liu J,Cui Z,Wang Y,Chen J,Chan HF,Tang S,Chen J

    更新日期:2020-05-01 00:00:00

  • Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration.

    abstract::A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Pars S,Cristo F,Inácio JM,Rosas G,Carreira IM,Melo JB,Mendes P,Martins DS,de Almeida LP,Maio J,Anjos R,Belo JA

    更新日期:2018-05-01 00:00:00

  • Generation of three induced pluripotent stem cell lines (MHHi012-A, MHHi013-A, MHHi014-A) from a family with Loeys-Dietz syndrome carrying a heterozygous p.M253I (c.759G>A) mutation in the TGFBR1 gene.

    abstract::Loeys-Dietz syndrome (LDS) is a rare connective tissue disorder characterized by a genetic predisposition for thoracic aortic aneurysm and dissection. Despite heterozygous loss-of-function mutations in genes for ligand, receptor, or downstream mediators of the transforming growth factor β (TGFβ) pathway, LDS is associ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Pongpamorn P,Dahlmann J,Haase A,Ebeling CT,Merkert S,Göhring G,Lachmann N,Martens A,Haverich A,Martin U,Olmer R

    更新日期:2020-03-01 00:00:00

  • Generation of induced pluripotent stem cell line (ZZUi004-A) from urine sample of a patient with spinocerebellar ataxia type 3.

    abstract::Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the region of the ATXN3 gene. The main feature of SCA3 is progressive ataxia, which affects balance, gait, and speech. Urine cells (UCs) of a SCA3 patient were successfully translated to induced...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Wang Y,Shi C,Wang Z,Sun H,Yang Z,Zhang F,Liu Y,Liu H,Jiang C,Zhang S,Xu Y,Wen X

    更新日期:2018-04-01 00:00:00

  • Cell division in the cerebral cortex of adult rats after photothrombotic ring stroke.

    abstract::Neurogenesis has been shown to occur in the cerebral cortex in adult rats after ischemic stroke. The origin of the newborn neurons is largely unknown. This study aimed to explore cell division in the poststroke penumbral cortex. Adult male Wistar rats were subjected to photothrombotic ring stroke. After repeated deliv...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Gu W,Brännström T,Rosqvist R,Wester P

    更新日期:2009-01-01 00:00:00

  • Integration of BMP/Wnt signaling to control clonal growth of limbal epithelial progenitor cells by niche cells.

    abstract::Both BMP and Wnt signaling control stem cells in bulge/dermal papilla, intestinal crypt, and bone marrow. To explore their roles in the limbal niche, which govern corneal epithelial homeostasis, we established an in vitro model of sphere growth by reunion between single limbal epithelial progenitor cells (LEPCs) and a...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Han B,Chen SY,Zhu YT,Tseng SC

    更新日期:2014-03-01 00:00:00

  • Derivation, characterization, and gene expression profile of two new human ES cell lines from India.

    abstract::Human embryonic stem cells (hESCs) offer new avenues for studying human development and disease progression in addition to their tremendous potential toward development of cell-replacement therapies for various cellular disorders. We have earlier reported the derivation and characterization of Relicell(®) hES1, the fi...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Mandal A,Bhowmik S,Patki A,Viswanathan C,Majumdar AS

    更新日期:2010-11-01 00:00:00