Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).


:The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.


Stem Cell Res


Stem cell research


Siehler J,Blöchinger AK,Akgün M,Wang X,Shahryari A,Geerlof A,Lickert H,Burtscher I




Has Abstract


2020-12-16 00:00:00












  • Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9.

    abstract::FOS is component of the AP-1 complex and has been reported to be involved in many cellular functions, including cell proliferation, differentiation, survival, angiogenesis, hematopoiesis and cancer progress. To further understand the exact role of FOS in these processes, here we created two FOS knockout human embryoni...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Li C,Wang Q,Peng Z,Lin Y,Liu H,Yang X,Li S,Liu X,Chen J

    更新日期:2019-08-01 00:00:00

  • Generation of patient-specific induced pluripotent stem cells from Leber's hereditary optic neuropathy.

    abstract::Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial disease caused by homoplasmic point mutations in complex I subunit genes of mitochondrial DNA. In this report, we generated an induced pluripotent stem cell (iPSCs) line, TVGH-iPSC-010-09, from the peripheral blood mononuclear cells of ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Lu HE,Yang YP,Chen YT,Wu YR,Wang CL,Tsai FT,Hwang DK,Lin TC,Chen SJ,Wang AG,Hsieh PCH,Chiou SH

    更新日期:2018-04-01 00:00:00

  • Micropatterning of human embryonic stem cells dissects the mesoderm and endoderm lineages.

    abstract::Human pluripotent cells such as human embryonic stem cells (hESC) are a great potential source of cells for cell-based therapies; however, directing their differentiation into the desired cell types with high purity remains a challenge. The stem cell microenvironment plays a vital role in directing hESC fate and we ha...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Lee LH,Peerani R,Ungrin M,Joshi C,Kumacheva E,Zandstra P

    更新日期:2009-03-01 00:00:00

  • A multiplexed screening method for pluripotency.

    abstract::Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Plotnikov A,Kozer N,Krupalnik V,Peles S,Mor N,Rais Y,Hanna JH,Barr HM

    更新日期:2017-08-01 00:00:00

  • Promotion of hematopoietic differentiation from mouse induced pluripotent stem cells by transient HoxB4 transduction.

    abstract::Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Tashiro K,Kawabata K,Omori M,Yamaguchi T,Sakurai F,Katayama K,Hayakawa T,Mizuguchi H

    更新日期:2012-03-01 00:00:00

  • Embryonic stem cell-derived motoneurons provide a highly sensitive cell culture model for botulinum neurotoxin studies, with implications for high-throughput drug discovery.

    abstract::Botulinum neurotoxins (BoNTs) inhibit cholinergic synaptic transmission by specifically cleaving proteins that are crucial for neurotransmitter exocytosis. Due to the lethality of these toxins, there are elevated concerns regarding their possible use as bioterrorism agents. Moreover, their widespread use for cosmetic ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Kiris E,Nuss JE,Burnett JC,Kota KP,Koh DC,Wanner LM,Torres-Melendez E,Gussio R,Tessarollo L,Bavari S

    更新日期:2011-05-01 00:00:00

  • Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro.

    abstract::Optimization of pluripotent stem cell expansion and differentiation is facilitated by biological tools that permit non-invasive and dynamic monitoring of pluripotency, and the ability to select for an undifferentiated input cell population. Here we report on the generation and characterisation of clonal human embryoni...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Ovchinnikov DA,Titmarsh DM,Fortuna PR,Hidalgo A,Alharbi S,Whitworth DJ,Cooper-White JJ,Wolvetang EJ

    更新日期:2014-09-01 00:00:00

  • Generation of two human induced pluripotent stem cell lines, UNIBSi012-A and UNIBSi013-A, from two patients with treatment-resistant depression.

    abstract::Novel and complementary experimental models are required for investigating the molecular mechanisms underlying the resistance to the available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third of patients and need to be deeply investigated. Here, we hav...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Bono F,Mutti V,Piovani G,Minelli A,Mingardi J,Guglielmi A,Fiorentini C,Barbon A,Missale C,Gennarelli M

    更新日期:2020-12-01 00:00:00

  • Predictive bioinformatics identifies novel regulators of proliferation in a cancer stem cell model.

    abstract::The cancer stem cell model postulates that tumors are hierarchically organized with a minor population, the cancer stem cells, exhibiting unlimited proliferative potential. These cells give rise to the bulk of tumor cells, which retain a limited ability to divide. Without successful targeting of cancer stem cells, tum...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Fields E,Wren JD,Georgescu C,Daum JR,Gorbsky GJ

    更新日期:2018-01-01 00:00:00

  • Generation of an integration-free induced pluripotent stem cell line (CSC-43) from a patient with sporadic Parkinson's disease.

    abstract::An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of com...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Marote A,Pomeshchik Y,Goldwurm S,Collin A,Lamas NJ,Pinto L,Salgado AJ,Roybon L

    更新日期:2018-03-01 00:00:00

  • Generation and characterization of two iPSC lines from human epicardium-derived cells.

    abstract::Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid bod...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Paulitschek C,Schulze-Matz P,Hesse J,Schmidt T,Wruck W,Adjaye J,Schrader J

    更新日期:2017-04-01 00:00:00

  • Generation of a human control iPSC line with a European mitochondrial haplogroup U background.

    abstract::Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus. ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Galera T,Zurita F,González-Páramos C,Moreno-Izquierdo A,Fraga MF,Fernández AF,Garesse R,Gallardo ME

    更新日期:2016-01-01 00:00:00

  • Generation of induced pluripotent stem cell line (ZZUi007-A) from a 52-year-old patient with a novel CHCHD2 gene mutation in Parkinson's disease.

    abstract::CHCHD2 mutation has been reported as a potential cause of a rare form of familial Parkinson's disease. Recently, a novel CHCHD2 mutation was identified in a family with Parkinson's disease. The dermal fibroblasts of the patient were obtained and successfully transformed into induced pluripotent stem cells(iPSCs), empl...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Wang Y,Wang Z,Sun H,Mao C,Yang J,Liu Y,Liu H,Zhang S,Zhang J,Xu Y,Shi C

    更新日期:2018-10-01 00:00:00

  • Establishment of an induced pluripotent stem cell line from a retinitis pigmentosa patient with compound heterozygous CRB1 mutation.

    abstract::The human iPSC line LEIi006-A was generated from dermal fibroblasts from a patient with retinitis pigmentosa using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. The iPSC cells carry compound heterozygous mutations (c.1892A > G and c.2548G > A) in the CRB1 gene. LEI...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Zhang X,Zhang D,Chen SC,Lamey T,Thompson JA,McLaren T,De Roach JN,Chen FK,McLenachan S

    更新日期:2018-08-01 00:00:00

  • Generation of three iPSC lines (IAIi002, IAIi004, IAIi003) from Rubinstein-Taybi syndrome 1 patients carrying CREBBP non sense c.4435G>T, p.(Gly1479*) and c.3474G>A, p.(Trp1158*) and missense c.4627G>T, p.(Asp1543Tyr) mutations.

    abstract::Rubinstein-Taybi syndrome (RSTS) is a neurodevelopmental disorder characterized by growth retardation, skeletal anomalies and intellectual disability, caused by heterozygous mutations in either CREBBP (RSTS1) or EP300 (RSTS2) genes. We characterized 3 iPSC lines generated by Sendai from blood of RSTS1 patients with un...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Alari V,Russo S,Rovina D,Garzo M,Crippa M,Calzari L,Scalera C,Concolino D,Castiglioni E,Giardino D,Prosperi E,Finelli P,Gervasini C,Gowran A,Larizza L

    更新日期:2019-10-01 00:00:00

  • An induced pluripotent stem cell line (TRNDi009-C) from a Niemann-Pick disease type A patient carrying a heterozygous p.L302P (c.905 T>C) mutation in the SMPD1 gene.

    abstract::Niemann-Pick disease type A (NPA) is a rare autosomal recessive lysosomal storage disease caused by mutations in the SMPD1 gene, which encodes for the protein acid sphingomyelinase. A human induced pluripotent stem cell (iPSC) line was generated from dermal fibroblasts of a 21-fetal-week-old female patient with NPA th...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Baskfield A,Li R,Beers J,Zou J,Liu C,Zheng W

    更新日期:2019-07-01 00:00:00

  • Integration of BMP/Wnt signaling to control clonal growth of limbal epithelial progenitor cells by niche cells.

    abstract::Both BMP and Wnt signaling control stem cells in bulge/dermal papilla, intestinal crypt, and bone marrow. To explore their roles in the limbal niche, which govern corneal epithelial homeostasis, we established an in vitro model of sphere growth by reunion between single limbal epithelial progenitor cells (LEPCs) and a...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Han B,Chen SY,Zhu YT,Tseng SC

    更新日期:2014-03-01 00:00:00

  • Cotransplantation of haploidentical hematopoietic and umbilical cord mesenchymal stem cells for severe aplastic anemia: successful engraftment and mild GVHD.

    abstract::Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunesuppressive effects. We cotransplan...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Wu Y,Cao Y,Li X,Xu L,Wang Z,Liu P,Yan P,Liu Z,Wang J,Jiang S,Wu X,Gao C,Da W,Han Z

    更新日期:2014-01-01 00:00:00

  • Generation of induced pluripotent stem cells (iPSCs) from an infant with Pompe disease carrying with compound mutations of R608X and E888X in GAA gene.

    abstract::Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a five months-old boy with glycogen storage disease type II(GSD II, also known as Pompe disease, PD) carries compound mutations R608X E888X in GAA gene. PBMCs were reprogrammed us...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Zhang Y,Li A,Wang J,Wang G,Wang D

    更新日期:2019-12-01 00:00:00

  • Establishment of induced pluripotent stem cell line (GSPHi001-A) from a 6-year old female with nephrotic syndrome.

    abstract::Peripheral blood mononuclear cells (PBMCs) were collected from a 6-year-old female child who was clinically diagnosed as primary nephrotic syndrome (NS) with hormone resistance. An iPSC line was successfully established by the Sendai-virus (SeV) delivery system. The iPS-19 (GSPHi001-A) expressed pluripotent markers, e...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Gao X,Gao X,Zhao H,Cui W,Tan M,Deng H

    更新日期:2019-12-01 00:00:00

  • Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes.

    abstract::We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) w...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Fatima A,Xu G,Nguemo F,Kuzmenkin A,Burkert K,Hescheler J,Šarić T

    更新日期:2016-09-01 00:00:00

  • Derivation of LIF-independent mouse iPS cells with modified Oct4.

    abstract::It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, ...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Hirai H,Firpo M,Kikyo N

    更新日期:2015-09-01 00:00:00

  • Feeder-independent derivation of induced-pluripotent stem cells from peripheral blood endothelial progenitor cells.

    abstract::Induced-pluripotent stem cells (iPSCs) are a potential alternative cell source in regenerative medicine, which includes the use of differentiated iPSCs for cell therapies to treat coronary artery and/or peripheral arterial diseases. Late-outgrowth endothelial progenitor cells (late-EPCs) are a unique primary cell pres...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Chang WY,Lavoie JR,Kwon SY,Chen Z,Manias JL,Behbahani J,Ling V,Kandel RA,Stewart DJ,Stanford WL

    更新日期:2013-03-01 00:00:00

  • Generation of an induced pluripotent stem cell line (CHFUi001-A) from an osteogenesis imperfecta patient with COL1A2 mutation.

    abstract::Osteogenesis Imperfecta (OI) is a rare autosomal dominant metabolic disorder caused by heterozygous mutations in the COL1A1 or COL1A2 genes, which encode the pro-α1(I) and pro-α2(I) chains of type I procollagen, respectively. A human induced pluripotent stem cell (iPSC) line, termed as CHFUi001-A, was generated from p...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Zheng Z,Lu W,Pei Z,Chen J,Yang T,Luo F

    更新日期:2020-07-05 00:00:00

  • Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation.

    abstract::Hypertrophic cardiomyopathy is an inherited cardiomyopathy with a prevalence of up to 1 in 200, which can result in significant morbidity and mortality. An iPSC line was generated from peripheral blood mononuclear cells obtained from the whole blood of a 58-year-old male with hypertrophic cardiomyopathy who carries th...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Holliday M,Ross SB,Lim S,Semsarian C

    更新日期:2018-12-01 00:00:00

  • Lymphoblast-derived integration-free iPSC lines from a female and male Alzheimer's disease patient expressing different copy numbers of a coding CNV in the Alzheimer risk gene CR1.

    abstract::Human lymphoblast cells from a female and male patient diagnosed with Alzheimer's disease (AD) with different genotypes of a functional copy number variation (CNV) in the AD risk gene CR1 were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal plasmids expressing OCT4, SOX2, NA...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Schröter F,Sleegers K,Van Cauwenberghe C,Bohndorf M,Wruck W,Van Broeckhoven C,Adjaye J

    更新日期:2016-11-01 00:00:00

  • An integration-free iPSC line SDQLCHi025-A from a girl with multiminicore disease carrying compound heterozygote mutations in RYR1 gene.

    abstract::Peripheral blood mononuclear cells for reprogramming in this work were donated by a girl with clinically and genetically diagnosed multiminicore disease harboring compound heterozygote mutations of RYR1 gene. Induced pluripotent stem cells (iPSCs) were obtained by non-integrating episomal vectors containing OCT4, SOX2...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Zhang H,Ma Y,Lv Y,Wan Y,Zhao Q,Gai Z,Liu Y

    更新日期:2020-05-01 00:00:00

  • HIF1α is a regulator of hematopoietic progenitor and stem cell development in hypoxic sites of the mouse embryo.

    abstract::Hypoxia affects many physiologic processes during early stages of mammalian ontogeny, particularly placental and vascular development. In the adult, the hypoxic bone marrow microenvironment plays a role in regulating hematopoietic stem cell (HSC) function. HSCs are generated from the major vasculature of the embryo, b...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Imanirad P,Solaimani Kartalaei P,Crisan M,Vink C,Yamada-Inagawa T,de Pater E,Kurek D,Kaimakis P,van der Linden R,Speck N,Dzierzak E

    更新日期:2014-01-01 00:00:00

  • Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies.

    abstract::Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, i...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Chevalier F,Lavergne M,Negroni E,Ferratge S,Carpentier G,Gilbert-Sirieix M,Siñeriz F,Uzan G,Albanese P

    更新日期:2014-05-01 00:00:00

  • Ocular surface ectoderm instigated by WNT inhibition and BMP4.

    abstract::We sought to elucidate how and when the ocular surface ectoderm commits to its differentiation into the corneal epithelium in eye development from human induced pluripotent stem cells (hiPSCs) under the influence of WNT signaling and the actions of BMP4. These signals are key drivers ocular surface ectodermal cell fat...

    journal_title:Stem cell research

    pub_type: 杂志文章


    authors: Kobayashi Y,Hayashi R,Shibata S,Quantock AJ,Nishida K

    更新日期:2020-07-01 00:00:00