Abstract:
:The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on matrigel-coated microcarriers achieving 2- to 4-fold higher cell densities than those in 2D colony cultures. Stable, continuous propagation of two hESC lines on microcarriers has been demonstrated in conditioned media for 6 months. Microcarrier cultures (MC) were also demonstrated in two serum-free defined media (StemPro and mTeSR1). MC achieved even higher cell concentrations in suspension spinner flasks, thus opening the prospect of propagation in controlled bioreactors.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Oh SK,Chen AK,Mok Y,Chen X,Lim UM,Chin A,Choo AB,Reuveny Sdoi
10.1016/j.scr.2009.02.005subject
Has Abstractpub_date
2009-05-01 00:00:00pages
219-30issue
3eissn
1873-5061issn
1876-7753pii
S1873-5061(09)00020-8journal_volume
2pub_type
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journal_title:Stem cell research
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journal_title:Stem cell research
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doi:10.1016/j.scr.2012.10.002
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journal_title:Stem cell research
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doi:10.1016/j.scr.2018.10.007
更新日期:2018-12-01 00:00:00
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doi:10.1016/j.scr.2020.102122
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pub_type: 杂志文章
doi:10.1016/j.scr.2016.01.013
更新日期:2016-03-01 00:00:00
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.01.007
更新日期:2016-03-01 00:00:00
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.05.008
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