Abstract:
:The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Zhou M,Wei R,Jiang Y,Fu J,Liu Y,Yang B,Yu B,Lin Y,Ran X,Lai WH,Chu M,Hu Y,Yang J,Tse HFdoi
10.1016/j.scr.2020.102120subject
Has Abstractpub_date
2020-12-10 00:00:00pages
102120eissn
1873-5061issn
1876-7753pii
S1873-5061(20)30421-9journal_volume
50pub_type
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