Generation of iPSC line from patient with arrhythmogenic right ventricular cardiomyopathy carrying mutations in PKP2 gene.

abstract：:Novel and complementary experimental models are required for investigating the molecular mechanisms underlying the resistance to the available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third of patients and need to be deeply investigated. Here, we hav...

journal_title：Stem cell research

authors： Bono F,Mutti V,Piovani G,Minelli A,Mingardi J,Guglielmi A,Fiorentini C,Barbon A,Missale C,Gennarelli M

更新日期：2020-12-01 00:00:00

abstract：:Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN t...

journal_title：Stem cell research

authors： Drick N,Sahabian A,Pongpamorn P,Merkert S,Göhring G,Welte T,Martin U,Olmer R

更新日期：2020-01-01 00:00:00

abstract：:Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprog...

journal_title：Stem cell research

authors： Haase A,Göhring G,Martin U

更新日期：2017-05-01 00:00:00

abstract：:An important practical limitation of the three-dimensional geometry of stem-cell derived intestinal organoids is that it prevents easy access to the apical epithelium for testing food components, microorganisms, bioactive and toxic compounds. To this end, we here report on a new robust method for generating confluent ...

journal_title：Stem cell research

authors： van der Hee B,Loonen LMP,Taverne N,Taverne-Thiele JJ,Smidt H,Wells JM

更新日期：2018-04-01 00:00:00

abstract：:Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG-repeat expanding mutation in ATXN3. We generated induced pluripotent stem cells (iPSCs) from a SCA3 patient by electroporation of dermal fibroblasts with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 an...

journal_title：Stem cell research

authors： Hansen SK,Borland H,Hasholt LF,Tümer Z,Nielsen JE,Rasmussen MA,Nielsen TT,Stummann TC,Fog K,Hyttel P

更新日期：2016-05-01 00:00:00

abstract：:We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 10years old patient with primary congenital glaucoma (PCG). The cells were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system and shown to have full differentiation potential. ...

journal_title：Stem cell research

authors： Zhang J,Wu S,Hu M,Liu Q

更新日期：2018-05-01 00:00:00

abstract：:Human pluripotent cells such as human embryonic stem cells (hESC) are a great potential source of cells for cell-based therapies; however, directing their differentiation into the desired cell types with high purity remains a challenge. The stem cell microenvironment plays a vital role in directing hESC fate and we ha...

journal_title：Stem cell research

authors： Lee LH,Peerani R,Ungrin M,Joshi C,Kumacheva E,Zandstra P

更新日期：2009-03-01 00:00:00

abstract：:An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency marker...

journal_title：Stem cell research

authors： Wang Y,Zhang J,Lei Y,Zhao J

更新日期：2018-12-01 00:00:00

abstract：:Glycogen debranching enzyme (GDE) plays a critical role in glycogenolysis. Mutations in the GDE gene are associated with a metabolic disease known as glycogen storage disease type III (GSDIII). We generated a mutant GDE human embryonic stem cell line, WAe001-A-14, using the CRISPR/Cas9 editing system. This cell line c...

journal_title：Stem cell research

authors： Xu G,Guo D,Wu F,Abbas N,Lai K,Yuan F,You K,Liu Y,Zhuang Y,Wu Y,Xu Y,Chen Y,Yang F,Pan T,Li YX

更新日期：2018-03-01 00:00:00

abstract：:Wolfram Syndrome is a rare, autosomal recessive genetic disorder with clinical symptoms appearing in early childhood. Here, we report a generation of iPSCs from fibroblasts of a patient affected by this disease. Induced pluripotent cells obtained with the application of integration-free episomal vectors display a norm...

journal_title：Stem cell research

authors： Grzela DP,Marciniak B,Pulaski L

更新日期：2020-07-01 00:00:00

abstract：:Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the region of the ATXN3 gene. The main feature of SCA3 is progressive ataxia, which affects balance, gait, and speech. Urine cells (UCs) of a SCA3 patient were successfully translated to induced...

journal_title：Stem cell research

authors： Wang Y,Shi C,Wang Z,Sun H,Yang Z,Zhang F,Liu Y,Liu H,Jiang C,Zhang S,Xu Y,Wen X

更新日期：2018-04-01 00:00:00

abstract：:Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus. ...

journal_title：Stem cell research

authors： Galera T,Zurita F,González-Páramos C,Moreno-Izquierdo A,Fraga MF,Fernández AF,Garesse R,Gallardo ME

更新日期：2016-01-01 00:00:00

abstract：:The Mesenchymal-to-Epithelial Transition (MET) has been recognized as a crucial step for successful reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs). Thus, it has been demonstrated, that the efficiency of reprogramming can be enhanced by promoting an epithelial expression program in cells, with a...

journal_title：Stem cell research

authors： Høffding MK,Hyttel P

更新日期：2015-01-01 00:00:00

abstract：:It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, ...

journal_title：Stem cell research

authors： Hirai H,Firpo M,Kikyo N

更新日期：2015-09-01 00:00:00

abstract：:The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated h...

journal_title：Stem cell research

authors： Siehler J,Blöchinger AK,Akgün M,Wang X,Shahryari A,Geerlof A,Lickert H,Burtscher I

更新日期：2020-12-16 00:00:00

abstract：:In order to evaluate the functional significance of palmitoylation during multi-potent neural stem/progenitor cell proliferation and differentiation, retinoic acid-induced P19 cells were used in this study as a model system. Cell behaviour was monitored in the presence of the protein palmitoylation inhibitor 2-bromopa...

journal_title：Stem cell research

authors： Chen X,Du Z,Shi W,Wang C,Yang Y,Wang F,Yao Y,He K,Hao A

更新日期：2014-03-01 00:00:00

abstract：:Neural progenitor cells (NPCs) are promising candidates for cell-based therapy of neurodegenerative diseases; however, safety concerns must be addressed through transplantation studies in large animal models, such as the pig. The aim of this study was to derive NPCs from porcine blastocysts and evaluate their in-vitro...

journal_title：Stem cell research

authors： Rasmussen MA,Hall VJ,Carter TF,Hyttel P

更新日期：2011-09-01 00:00:00

abstract：:We generated an induced human pluripotent stem cell line from a child with microcephaly carrying compound heterozygous mutations of TYW1 inherited from healthy parents. This iPS cell line showed typical embryonic stem cell-like morphology, expressed pluripotent markers that comparable to human embryonic stem cells. Mo...

journal_title：Stem cell research

authors： Sun C,Zhou P,Yuan S,Guo R,Zhang Z,Xu Q,Han D,He L,Tang H,Xu K,Hu H,Li N

更新日期：2020-05-01 00:00:00

abstract：:Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of m...

journal_title：Stem cell research

authors： Manukjan G,Ripperger T,Venturini L,Stadler M,Göhring G,Schambach A,Schlegelberger B,Steinemann D

更新日期：2016-05-01 00:00:00

abstract：:Insulin-like growth factor 1 (IGF-1) plays an important role in the regulation of tooth root development, and stem cells from apical papilla (SCAPs) are responsible for the formation of root pulp and dentin. To date, it remains unclear whether IGF-1 can regulate the function of SCAPs. In this study, SCAPs were isolate...

journal_title：Stem cell research

authors： Wang S,Mu J,Fan Z,Yu Y,Yan M,Lei G,Tang C,Wang Z,Zheng Y,Yu J,Zhang G

更新日期：2012-05-01 00:00:00

abstract：:Gastrointestinal (GI) homeostasis requires the action of multiple pathways. There is some controversy regarding whether small intestine (SI) Paneth cells (PCs) play a central role in orchestrating crypt architecture and their relationship with Lgr5+ve stem cells. Nevertheless, we previously showed that germline CSF-1 ...

journal_title：Stem cell research

authors： Akcora D,Huynh D,Lightowler S,Germann M,Robine S,de May JR,Pollard JW,Stanley ER,Malaterre J,Ramsay RG

更新日期：2013-03-01 00:00:00

abstract：:The discovery of the Ten-Eleven Translocation (TET) protein family was initiated by the identification of the MLL partner TET1, and of mutations in the TET2 gene in hematological malignancies including myeloproliferative neoplasms (MPN). TET1, 2 and 3 proteins hydroxylate 5-methylcytosine (5-mC) into 5-hydroxymethylcy...

journal_title：Stem cell research

authors： Secardin L,Limia CEG,di Stefano A,Bonamino MH,Saliba J,Kataoka K,Rehen SK,Raslova H,Marty C,Ogawa S,Vainchenker W,Monte-Mor BDCR,Plo I

更新日期：2020-04-01 00:00:00

abstract：:Among the known causative genes of familial ALS, SOD1mutation is one of the most common. It encodes for the ubiquitous detoxifying copper/zinc binding SOD1 enzyme, whose mutations selectively cause motor neuron death, although the mechanisms are not as yet clear. What is known is that mutant-mediated toxicity is not c...

journal_title：Stem cell research

authors： D'Anzi A,Altieri F,Perciballi E,Ferrari D,Bernardini L,Goldoni M,Mazzini L,De Marchi F,Di Pierro A,D'Alfonso S,Gelati M,Vescovi AL,Rosati J

更新日期：2020-07-25 00:00:00

abstract：:The purpose of this study is to establish a murine embryonic stem cell (mESC) line for isolation of functional ventricular cardiomyocytes (VCMs) and then to characterize the derived VCMs. By crossing the myosin light chain 2v (Mlc2v)-Cre mouse line with the reporter strain Rosa26-yellow fluorescent protein (YFP), we g...

journal_title：Stem cell research

authors： Lee MY,Sun B,Schliffke S,Yue Z,Ye M,Paavola J,Bozkulak EC,Amos PJ,Ren Y,Ju R,Jung YW,Ge X,Yue L,Ehrlich BE,Qyang Y

更新日期：2012-01-01 00:00:00

abstract：:Characterisation of mouse pluripotent stem cells has revealed two distinct pluripotent states, naive and primed, that maintain characteristics of the pre and post implanted epiblast respectively. Recent studies have developed several culture systems that seek to recapitulate the naive phenomenon in human pluripotent s...

journal_title：Stem cell research

authors： Trusler O,Huang Z,Goodwin J,Laslett AL

更新日期：2018-01-01 00:00:00

abstract：:Maturity onset diabetes of the young (MODY) is a hereditary form of diabetes mellitus presenting at childhood or adolescence, which eventually leads to pancreatic β-cells dysfunction. The underlying genetic basis of MODY disorders is haploinsufficiency, where loss-of-function mutations in a single allele cause the dia...

journal_title：Stem cell research

authors： Braverman-Gross C,Nudel N,Ronen D,Beer NL,McCarthy MI,Benvenisty N

更新日期：2018-08-01 00:00:00

abstract：:The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under ...

journal_title：Stem cell research

authors： Devito L,Jacquet L,Petrova A,Miere C,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

更新日期：2016-01-01 00:00:00

abstract：:Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, i...

journal_title：Stem cell research

authors： Chevalier F,Lavergne M,Negroni E,Ferratge S,Carpentier G,Gilbert-Sirieix M,Siñeriz F,Uzan G,Albanese P

更新日期：2014-05-01 00:00:00

abstract：:Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous kn...

journal_title：Stem cell research

authors： Zhang T,Huang W,Xue X

更新日期：2020-12-10 00:00:00

abstract：:Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into in...

journal_title：Stem cell research

authors： Ciampi O,Iacone R,Longaretti L,Benedetti V,Graf M,Magnone MC,Patsch C,Xinaris C,Remuzzi G,Benigni A,Tomasoni S

更新日期：2016-07-01 00:00:00