Abstract：
:The discovery of the Ten-Eleven Translocation (TET) protein family was initiated by the identification of the MLL partner TET1, and of mutations in the TET2 gene in hematological malignancies including myeloproliferative neoplasms (MPN). TET1, 2 and 3 proteins hydroxylate 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC) and further oxidize 5-hmC into 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC). Previous studies highlight the involvement of TET proteins in somatic cells reprogramming into induced pluripotent stem cells (iPSC), particularly Tet1 and 2 in mouse and TET1 in human. Here, we asked whether endogenous TET2 knockdown also displays this function. Using different shRNA against TET2, we provide evidence that TET2 strongly decreases the reprogramming of human hematopoietic progenitor cells into iPSC. Importantly, using 2 MPN patients, we observed that TET2 mutations affecting catalytic domain allowed iPSC generation. Instead, using another TET2 and TET3-mutated patient, we could only reprogram IPSC with TET3 mutation alone, suggesting that the type of TET2 mutation and/or the cooperation with TET3 mutations may alter the reprogramming activity. Altogether, this work highlights the importance of endogenous TET in the reprogramming process of human hematopoietic progenitors.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Secardin L,Limia CEG,di Stefano A,Bonamino MH,Saliba J,Kataoka K,Rehen SK,Raslova H,Marty C,Ogawa S,Vainchenker W,Monte-Mor BDCR,Plo Idoi
10.1016/j.scr.2020.101755subject
Has Abstractpub_date
2020-04-01 00:00:00pages
101755eissn
1873-5061issn
1876-7753pii
S1873-5061(20)30059-3journal_volume
44pub_type
杂志文章abstract：:Incomplete achromatopsia (ACHM) is a disorder in which there is function defect of cone photoreceptors in the retina and individuals with such disease retain residual color vision. Here, we have generated an induced pluripotent stem cell (iPSC) line carrying a homozygous mutation in the PDE6C gene, already related wit...
journal_title：Stem cell research
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abstract：:The KCL037 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under ...
journal_title：Stem cell research
pub_type： 杂志文章
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abstract：BACKGROUND:Angiogenesis is an essential step in tissue engineering. MSC exosomes play an important role in angiogenesis. Functional biomolecules in exosomes vested by the culture microenvironment can be transferred to recipient cells and affects their effect. 3D culture can improve the proliferation and activity of MSC...
journal_title：Stem cell research
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abstract：:Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disordersmay yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a ne...
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.04.012
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journal_title：Stem cell research
pub_type： 杂志文章
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.09.001
更新日期：2012-03-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Stem cell research
pub_type： 杂志文章
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.04.003
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.05.007
更新日期：2018-08-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.05.004
更新日期：2018-07-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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journal_title：Stem cell research
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pub_type： 杂志文章
doi：10.1016/j.scr.2015.04.008
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2013-09-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Stem cell research
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更新日期：2020-12-10 00:00:00
abstract：:Loeys-Dietz syndrome (LDS) is a rare connective tissue disorder characterized by a genetic predisposition for thoracic aortic aneurysm and dissection. Despite heterozygous loss-of-function mutations in genes for ligand, receptor, or downstream mediators of the transforming growth factor β (TGFβ) pathway, LDS is associ...
journal_title：Stem cell research
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2017-08-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102126
更新日期：2020-12-16 00:00:00
abstract：:Human embryonic stem cells (hESCs) can undergo unlimited self-renewal and differentiate into hepatic cells, including expandable hepato-blasts (HBs) and hepatocyte-like cells (HLCs) in vitro. Therefore, hESC-derived HBs have the potential to become a renewable cell source for cell therapy of serious liver damage. Howe...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102010
更新日期：2020-12-01 00:00:00
abstract：:Progenitor cells in the adult pancreas are potential sources of endocrine beta cells for treating type 1 diabetes. Previously, we identified tri-potent progenitor cells in the adult (2-4month-old) murine pancreas that were capable of self-renewal and differentiation into duct, acinar, and endocrine cells in vitro. The...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.11.015
更新日期：2016-01-01 00:00:00
