Abstract：
:Progenitor cells in the adult pancreas are potential sources of endocrine beta cells for treating type 1 diabetes. Previously, we identified tri-potent progenitor cells in the adult (2-4month-old) murine pancreas that were capable of self-renewal and differentiation into duct, acinar, and endocrine cells in vitro. These progenitor cells were named pancreatic colony-forming units (PCFUs). However, because PCFUs are a minor population in the pancreas (~1%) they are difficult to study. To enrich PCFUs, strategies using cell-surface marker analyses and fluorescence-activated cell sorting were developed. We found that CD133(high)CD71(low) cells, but not other cell populations, enriched PCFUs by up to 30 fold compared to the unsorted cells. CD133(high)CD71(low) cells generated primary, secondary, and subsequent colonies when serially re-plated in Matrigel-containing cultures, suggesting self-renewal abilities. In the presence of a laminin hydrogel, CD133(high)CD71(low) cells gave rise to colonies that contained duct, acinar, and Insulin(+)Glucagon(+) double-hormonal endocrine cells. Colonies from the laminin hydrogel culture were implanted into diabetic mice, and five weeks later duct, acinar, and Insulin(+)Glucagon(-) cells were detected in the grafts, demonstrating tri-lineage differentiation potential of CD133(high)CD71(low) cells. These CD133(high)CD71(low) cells will enable future studies of putative adult pancreas stem cells in vivo.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Jin L,Gao D,Feng T,Tremblay JR,Ghazalli N,Luo A,Rawson J,Quijano JC,Chai J,Wedeken L,Hsu J,LeBon J,Walker S,Shih HP,Mahdavi A,Tirrell DA,Riggs AD,Ku HTdoi
10.1016/j.scr.2015.11.015subject
Has Abstractpub_date
2016-01-01 00:00:00pages
40-53issue
1eissn
1873-5061issn
1876-7753pii
S1873-5061(15)00168-3journal_volume
16pub_type
杂志文章abstract：:An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of com...
journal_title：Stem cell research
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pub_type： 杂志文章,评审
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journal_title：Stem cell research
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abstract：:Neurogenesis has been shown to occur in the cerebral cortex in adult rats after ischemic stroke. The origin of the newborn neurons is largely unknown. This study aimed to explore cell division in the poststroke penumbral cortex. Adult male Wistar rats were subjected to photothrombotic ring stroke. After repeated deliv...
journal_title：Stem cell research
pub_type： 杂志文章
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abstract：:Asparagine synthetase (ASNS) deficiency (ASNSD; MIM #615574) is a rare neurodevelopmental disorder caused by mutations in the ASNS gene. The ASNS gene maps to cytogenetic band 7q21.3 and is 35 kb long. ASNSD is characterised by congenital microcephaly, severely delayed psychomotor development, seizures, and hyperekple...
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abstract：:Mesenchymal stem cells (MSC) have been isolated from almost every adult tissue. In cord blood (CB), different non-hematopoietic CD45-, CD34- adherent cell populations can be generated: the cord blood derived MSC (CB-MSC), that behave almost like MSC from bone marrow (BM-MSC), and unrestricted somatic stem cells (USSC)...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2010.03.004
更新日期：2010-07-01 00:00:00
abstract：:The purpose of this study is to establish a murine embryonic stem cell (mESC) line for isolation of functional ventricular cardiomyocytes (VCMs) and then to characterize the derived VCMs. By crossing the myosin light chain 2v (Mlc2v)-Cre mouse line with the reporter strain Rosa26-yellow fluorescent protein (YFP), we g...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.08.004
更新日期：2012-01-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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abstract：BACKGROUND:Non-random segregation of DNA strands during stem cell replication has been proposed as a mechanism to minimize accumulated genetic errors in stem cells of rapidly dividing tissues. According to this hypothesis, an "immortal" DNA strand is passed to the stem cell daughter and not the more differentiated cell...
journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2015-03-01 00:00:00
abstract：:The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propag...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.01.007
更新日期：2016-03-01 00:00:00
abstract：:Bone marrow stromal cell (BMSC) adhesion and migration are fundamental to a number of pathophysiologic processes, including fracture and wound healing. Vitamin C is beneficial for bone formation, fracture repair and wound healing. However, the role of the vitamin C transporter in BMSC adhesion, migration and wound hea...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2013.11.002
更新日期：2014-03-01 00:00:00
abstract：:The human embryonic stem cell line NYSCFe001-A was derived from a day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe001-A line, registered as NYSCF100 on the NIH reg...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.03.017
更新日期：2018-05-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.09.003
更新日期：2017-10-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2018-10-01 00:00:00
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更新日期：2014-07-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
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journal_title：Stem cell research
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journal_title：Stem cell research
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abstract：:Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, s...
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更新日期：2019-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-07-01 00:00:00
abstract：:Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of esta...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.05.003
更新日期：2016-07-01 00:00:00
