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Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1.

Abstract：

:ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Wen MH,Xie X,Tu J,Lee DF,Chen TY

doi

10.1016/j.scr.2019.101631

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

101631

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(19)30261-2

journal_volume

41

pub_type

杂志文章

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