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Generation of OCT4-EGFP, NANOG-tdTomato dual reporter human induced pluripotent stem cell line, KKUi001-A, using the CRISPR/Cas9 system.

Abstract：

:OCT4 and NANOG are core transcription factor genes in self-renewal, differentiation, and reprogramming. Here, we generated an OCT4-EGFP, NANOG-tdTomato dual reporter hiPSC line, KKUi001-A, on the basis of human induced pluripotent stem cells using CRISPR/Cas9 technology. EGFP and tdTomato reporter were inserted into before the stop codon of OCT4 and NANOG, respectively. Simultaneous expression of EGFP and tdTomato was observed when expression of OCT4 and NANOG was changed during differentiation and reprogramming. KKUi001-A hiPSC line will be a useful tool to find initial time point of OCT4 and NANOG expression during reprogramming process and to screen small molecules that promote reprogramming.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Lee M,Choi NY,Park S,Bang JS,Lee Y,Jeong D,Ham S,Lim S,Kim KH,Ko K

doi

10.1016/j.scr.2020.101943

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

101943

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(20)30244-0

journal_volume

48

pub_type

杂志文章

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