Abstract：
:Human embryonic stem cells (hESCs) can undergo unlimited self-renewal and differentiate into hepatic cells, including expandable hepato-blasts (HBs) and hepatocyte-like cells (HLCs) in vitro. Therefore, hESC-derived HBs have the potential to become a renewable cell source for cell therapy of serious liver damage. However, one of the key challenges for such cell therapy is the allogeneic immune rejection of hESC-derived HBs. To overcome this challenge, we developed a strategy to protect the hESC-derived HBs from allogeneic immune rejection by ectopically expressing immune suppressive molecules CTLA4-Ig and PD-L1, denoted CP HBs. Like HBs derived from normal hESCs, CP HBs are capable of repairing liver damage in animal models. Using humanized mice (Hu-mice) reconstituted with human immune system, we showed that CP HBs are protected from allogeneic immune system and can survive long-term in Hu-mice. These data support the feasibility to develop CP HBs into a cell therapy to treat serious liver damage.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Liu J,Pan T,Chen Y,Liu Y,Yang F,Chen Q,Abbas N,Zhong M,Zhang Q,Xu Y,Li YXdoi
10.1016/j.scr.2020.102010subject
Has Abstractpub_date
2020-12-01 00:00:00pages
102010eissn
1873-5061issn
1876-7753pii
S1873-5061(20)30311-1journal_volume
49pub_type
杂志文章abstract：:Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, s...
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