Abstract:
:Autosomal recessive Stargardt disease is the most common cause of inherited retinal disease. In this report, we describe the generation and characterization of two human induced pluripotent stem cell (iPSC) lines from a patient with compound heterozygous mutations in the ABCA4 gene (c.[768G>T];[6079C>T]). Patient dermal fibroblasts were reprogrammed using episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for P53. The clonal iPSC lines LEIi012-A and LEIi012-B were established. Both lines had a normal karyotype, displayed iPSC morphology, expressed pluripotency genes at similar levels to control iPSC and displayed trilineage differentiation potential during embryoid body differentiation.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Jennings L,Zhang D,Chen SC,Moon SY,Lamey T,Thompson JA,McLaren T,De Roach JN,Chen FK,McLenachan Sdoi
10.1016/j.scr.2020.101947subject
Has Abstractpub_date
2020-10-01 00:00:00pages
101947eissn
1873-5061issn
1876-7753pii
S1873-5061(20)30248-8journal_volume
48pub_type
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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doi:10.1016/j.scr.2019.101443
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101631
更新日期:2019-12-01 00:00:00
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章,评审
doi:10.1016/j.scr.2011.11.001
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abstract::There are currently no reports of identification of stem cells in human gallbladder. The differences between human gallbladder and intrahepatic bile duct (IHBD) cells have also not been explored. The goals of this study were to evaluate if human fetal gallbladder contains a candidate stem cell population and if fetal ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.12.003
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