Cardiac regeneration based on mechanisms of cardiomyocyte proliferation and differentiation.

abstract：:GADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been me...

journal_title：Stem cell research

authors： Li J,Li Y,Zhan XY,Ran X,Tse HF,Dang S,Nie Y,Huang K

更新日期：2020-12-01 00:00:00

abstract：:The Global Alliance for iPSC Therapies (GAiT) is a new initiative to support the implementation and clinical application of therapies derived from pluripotent stem cells to the benefit of patients globally. GAiT's mission is to serve as a central, international resource for those organisations developing therapies fro...

journal_title：Stem cell research

authors： Sullivan S,Ginty P,McMahon S,May M,Solomon SL,Kurtz A,Stacey GN,Bennaceur Griscelli A,Li RA,Barry J,Song J,Turner ML

更新日期：2020-12-01 00:00:00

abstract：:We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripote...

journal_title：Stem cell research

authors： Liu CL,Huang CY,Chen HC,Lu HE,Hsieh PCH,Lee JJ

更新日期：2020-05-01 00:00:00

abstract：:Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunesuppressive effects. We cotransplan...

journal_title：Stem cell research

authors： Wu Y,Cao Y,Li X,Xu L,Wang Z,Liu P,Yan P,Liu Z,Wang J,Jiang S,Wu X,Gao C,Da W,Han Z

更新日期：2014-01-01 00:00:00

abstract：:Here we utilized the chromatin in vivo assay (CiA) mouse platform to directly examine the epigenetic barriers impeding the activation of the CiA:Oct4 allele in mouse embryonic fibroblasts (MEF)s when stimulated with a transcription factor. The CiA:Oct4 allele contains an engineered EGFP reporter replacing one copy of ...

journal_title：Stem cell research

authors： Headley KM,Kedziora KM,Alejo A,Lai EZ,Purvis JE,Hathaway NA

更新日期：2019-07-01 00:00:00

abstract：:Gene therapy mediated by bone marrow-derived hematopoietic stem cells (BM-HSC) has been widely used in treating genetic deficiencies in both pre-clinical and clinical settings. Using mitotically inactive cell-targeting lentivirus with separate promoters for our gene of interest (the murine MHC class II (MHCII) chapero...

journal_title：Stem cell research

authors： Wang N,Rajasekaran N,Hou T,Mellins ED

更新日期：2014-01-01 00:00:00

abstract：:Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...

journal_title：Stem cell research

authors： Zhang C,Yu Y,Li F,Lan X,Wang L

更新日期：2020-12-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) offer new avenues for studying human development and disease progression in addition to their tremendous potential toward development of cell-replacement therapies for various cellular disorders. We have earlier reported the derivation and characterization of Relicell(®) hES1, the fi...

journal_title：Stem cell research

authors： Mandal A,Bhowmik S,Patki A,Viswanathan C,Majumdar AS

更新日期：2010-11-01 00:00:00

abstract：:Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous kn...

journal_title：Stem cell research

authors： Zhang T,Huang W,Xue X

更新日期：2020-12-10 00:00:00

abstract：:Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. He...

journal_title：Stem cell research

authors： Hong SH,Lee MH,Koo MA,Seon GM,Park YJ,Kim D,Park JC

更新日期：2019-07-01 00:00:00

abstract：:The human iPSC line LEIi006-A was generated from dermal fibroblasts from a patient with retinitis pigmentosa using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. The iPSC cells carry compound heterozygous mutations (c.1892A > G and c.2548G > A) in the CRB1 gene. LEI...

journal_title：Stem cell research

authors： Zhang X,Zhang D,Chen SC,Lamey T,Thompson JA,McLaren T,De Roach JN,Chen FK,McLenachan S

更新日期：2018-08-01 00:00:00

abstract：:Human iPSC line, iPSC-ADM01(SYSUi001-A), was generated from a 70-year-old male patient with sporadic Alzheimer's disease, using non-integrative reprogramming method. This cell line shows pluripotency both in vitro and in vivo, and has a normal karyotype. ...

journal_title：Stem cell research

authors： Wei R,Han H,Ye M,He L,Lei Q,Zhou T,Cai X,Li Z

更新日期：2019-03-01 00:00:00

abstract：:Liver cirrhosis accompanied with hepatic encephalopathy commonly causes cognitive impairment in patients. To model this disease, two independent patient specific induced pluripotent stem cell-line (iPSC) clones, NCCSi011-A and NCCSi011-B were generated by reprogramming the CD4+ T cells of an Indian male patient suffer...

journal_title：Stem cell research

authors： Vaidyanath A,Khan M,Vaishnav B,Kakrani AL,Patil S,Shiras A

更新日期：2020-10-01 00:00:00

abstract：:The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on ma...

journal_title：Stem cell research

authors： Oh SK,Chen AK,Mok Y,Chen X,Lim UM,Chin A,Choo AB,Reuveny S

更新日期：2009-05-01 00:00:00

abstract：:It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, ...

journal_title：Stem cell research

authors： Hirai H,Firpo M,Kikyo N

更新日期：2015-09-01 00:00:00

abstract：:The cancer stem cell model postulates that tumors are hierarchically organized with a minor population, the cancer stem cells, exhibiting unlimited proliferative potential. These cells give rise to the bulk of tumor cells, which retain a limited ability to divide. Without successful targeting of cancer stem cells, tum...

journal_title：Stem cell research

authors： Fields E,Wren JD,Georgescu C,Daum JR,Gorbsky GJ

更新日期：2018-01-01 00:00:00

abstract：:Two distinct types of embryonic pluripotent stem cells can be established from either the inner cell mass (ICM) of preimplantation blastocyst (leukemia inhibitory factor (LIF)-dependent embryonic stem cell, ESC, called naive state) or the epiblast of postimplantation fetuses (fibroblast growth factor 2 (FGF2)-dependen...

journal_title：Stem cell research

authors： Ozawa M,Kawakami E,Sakamoto R,Shibasaki T,Goto A,Yoshida N

更新日期：2014-07-01 00:00:00

abstract：:ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endoge...

journal_title：Stem cell research

authors： Wen MH,Xie X,Tu J,Lee DF,Chen TY

更新日期：2019-12-01 00:00:00

abstract：:Our work aimed at evaluating the role of adipose stem cells (ASC) on chondrocytes from osteoarthritic (OA) patients and identifying the mediators involved. We used primary chondrocytes, ASCs from different sources and bone marrow mesenchymal stromal cells (MSC) from OA donors. ASCs or MSCs were co-cultured with chondr...

journal_title：Stem cell research

authors： Maumus M,Manferdini C,Toupet K,Peyrafitte JA,Ferreira R,Facchini A,Gabusi E,Bourin P,Jorgensen C,Lisignoli G,Noël D

更新日期：2013-09-01 00:00:00

abstract：:The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurg...

journal_title：Stem cell research

authors： Miere C,Hewitson H,Devito L,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

更新日期：2016-03-01 00:00:00

abstract：:The human embryonic stem cell line RCe019-A (RC-15) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a cleavage st...

journal_title：Stem cell research

authors： De Sousa PA,Tye BJ,Bruce K,Dand P,Russell G,Collins DM,Greenshields A,McDonald K,Bradburn H,Laurie A,Downie JM,Bateman M,Courtney A

更新日期：2016-05-01 00:00:00

abstract：:Liver progenitor (oval) cells have enormous potential in the treatment of patients with liver disease using a cell therapy approach, but their use is limited by their scarcity and the number of donor livers from which they can be derived. Bone marrow may be a suitable source. Previously the derivation of oval cells fr...

journal_title：Stem cell research

authors： Tonkin JN,Knight B,Curtis D,Abraham LJ,Yeoh GC

更新日期：2008-09-01 00:00:00

abstract：:There are currently no reports of identification of stem cells in human gallbladder. The differences between human gallbladder and intrahepatic bile duct (IHBD) cells have also not been explored. The goals of this study were to evaluate if human fetal gallbladder contains a candidate stem cell population and if fetal ...

journal_title：Stem cell research

authors： Manohar R,Li Y,Fohrer H,Guzik L,Stolz DB,Chandran UR,LaFramboise WA,Lagasse E

更新日期：2015-05-01 00:00:00

abstract：:The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1...

journal_title：Stem cell research

authors： Zhou M,Wei R,Jiang Y,Fu J,Liu Y,Yang B,Yu B,Lin Y,Ran X,Lai WH,Chu M,Hu Y,Yang J,Tse HF

更新日期：2020-12-10 00:00:00

abstract：:Glycogen debranching enzyme (GDE) plays a critical role in glycogenolysis. Mutations in the GDE gene are associated with a metabolic disease known as glycogen storage disease type III (GSDIII). We generated a mutant GDE human embryonic stem cell line, WAe001-A-14, using the CRISPR/Cas9 editing system. This cell line c...

journal_title：Stem cell research

authors： Xu G,Guo D,Wu F,Abbas N,Lai K,Yuan F,You K,Liu Y,Zhuang Y,Wu Y,Xu Y,Chen Y,Yang F,Pan T,Li YX

更新日期：2018-03-01 00:00:00

abstract：:Induced-pluripotent stem cells (iPSCs) are a potential alternative cell source in regenerative medicine, which includes the use of differentiated iPSCs for cell therapies to treat coronary artery and/or peripheral arterial diseases. Late-outgrowth endothelial progenitor cells (late-EPCs) are a unique primary cell pres...

journal_title：Stem cell research

authors： Chang WY,Lavoie JR,Kwon SY,Chen Z,Manias JL,Behbahani J,Ling V,Kandel RA,Stewart DJ,Stanford WL

更新日期：2013-03-01 00:00:00

abstract：:Friedreich's ataxia is caused by large homozygous, intronic expansions of GAA repeats in the frataxin (FXN) gene, resulting in severe downregulation of its expression. Pathogenic repeats are located in intron one, hence patients express unaffected FXN protein, albeit in low quantities. Although FRDA symptoms typically...

journal_title：Stem cell research

authors： Li J,Rozwadowska N,Clark A,Fil D,Napierala JS,Napierala M

更新日期：2019-10-01 00:00:00

abstract：:Constant neuroregeneration in adult olfactory epithelium maintains olfactory function by basal stem cell proliferation and differentiation to replace lost olfactory sensory neurons (OSNs). Understanding the mechanisms regulating this process could reveal potential therapeutic targets for stimulating adult olfactory ne...

journal_title：Stem cell research

authors： Jia C,Oliver J,Gilmer D,Lovins C,Rodriguez-Gil DJ,Hagg T

更新日期：2020-12-01 00:00:00

abstract：:Neural progenitor cells have been proposed as a therapy for central nervous system disorders, including neurodegenerative diseases and trauma injuries, however their accessibility is a major limitation. We recently isolated Tuj1+ cells from skeletal muscle culture of Nestin-GFP transgenic mice however whether they for...

journal_title：Stem cell research

authors： Birbrair A,Zhang T,Wang ZM,Messi ML,Enikolopov GN,Mintz A,Delbono O

更新日期：2013-01-01 00:00:00

abstract：:The CD71/Ter119 combination has been widely used to reflect dynamic maturation of erythrocytes in vivo. However, because CD71 is expressed on all proliferating cells, it is unclear whether it can be utilized as an erythrocyte-specific marker during differentiation of embryonic stem cells (ESCs). In this study, we reve...

journal_title：Stem cell research

authors： Chao R,Gong X,Wang L,Wang P,Wang Y

更新日期：2015-01-01 00:00:00