Abstract:
:Human iPSC line, iPSC-ADM01(SYSUi001-A), was generated from a 70-year-old male patient with sporadic Alzheimer's disease, using non-integrative reprogramming method. This cell line shows pluripotency both in vitro and in vivo, and has a normal karyotype.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Wei R,Han H,Ye M,He L,Lei Q,Zhou T,Cai X,Li Zdoi
10.1016/j.scr.2018.101375subject
Has Abstractpub_date
2019-03-01 00:00:00pages
101375eissn
1873-5061issn
1876-7753pii
S1873-5061(18)30310-6journal_volume
35pub_type
杂志文章abstract::We sought to elucidate how and when the ocular surface ectoderm commits to its differentiation into the corneal epithelium in eye development from human induced pluripotent stem cells (hiPSCs) under the influence of WNT signaling and the actions of BMP4. These signals are key drivers ocular surface ectodermal cell fat...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101868
更新日期:2020-07-01 00:00:00
abstract::Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them in a medium supplemented with fetal calf serum and leukemia inhi...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.09.001
更新日期:2017-10-01 00:00:00
abstract::The human iPSC line LEIi006-A was generated from dermal fibroblasts from a patient with retinitis pigmentosa using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. The iPSC cells carry compound heterozygous mutations (c.1892A > G and c.2548G > A) in the CRB1 gene. LEI...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.08.001
更新日期:2018-08-01 00:00:00
abstract::Interactions between acute myeloid leukemia (AML) blasts and neighboring stromal cells are important for disease development and chemosensitivity. However, the molecular mechanisms involved in the cytokine-mediated crosstalk between mesenchymal stem cells (MSCs) and AML cells are largely unknown. Leukemic cells derive...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.09.008
更新日期:2015-11-01 00:00:00
abstract::Neural progenitor cells (NPCs) are promising candidates for cell-based therapy of neurodegenerative diseases; however, safety concerns must be addressed through transplantation studies in large animal models, such as the pig. The aim of this study was to derive NPCs from porcine blastocysts and evaluate their in-vitro...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2011.04.004
更新日期:2011-09-01 00:00:00
abstract::The discovery of the Ten-Eleven Translocation (TET) protein family was initiated by the identification of the MLL partner TET1, and of mutations in the TET2 gene in hematological malignancies including myeloproliferative neoplasms (MPN). TET1, 2 and 3 proteins hydroxylate 5-methylcytosine (5-mC) into 5-hydroxymethylcy...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101755
更新日期:2020-04-01 00:00:00
abstract::Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.12.026
更新日期:2017-03-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 60-year-old female diagnosed with sporadic amyotrophic lateral sclerosis (sALS). The iPSCs shared the same karyotype with the parent PBMCs, expressed pluripotency stem cell markers, and demonstrated tr...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101841
更新日期:2020-05-01 00:00:00
abstract::SLC26A4 is the second most frequent gene implicated in congenital hearing loss after GJB2 mutations. Here, we report the generation of induced pluripotent stem cells (iPSCs), from a patient who was carrying a homozygous c.919-2A>G variant in the SLC26A4 gene. This is the most common variant of SLC26A4 gene in the Chin...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101524
更新日期:2019-10-01 00:00:00
abstract::Cardiomyocyte proliferation and progenitor differentiation are endogenous mechanisms of myocardial development. Cardiomyocytes continue to proliferate in mammals for part of post-natal development. In adult mammals under homeostatic conditions, cardiomyocytes proliferate at an extremely low rate. Because the mechanism...
journal_title:Stem cell research
pub_type: 杂志文章,评审
doi:10.1016/j.scr.2014.09.003
更新日期:2014-11-01 00:00:00
abstract::Peripheral blood mononuclear cells (PBMCs) were collected from a 6-year-old female child who was clinically diagnosed as primary nephrotic syndrome (NS) with hormone resistance. An iPSC line was successfully established by the Sendai-virus (SeV) delivery system. The iPS-19 (GSPHi001-A) expressed pluripotent markers, e...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101661
更新日期:2019-12-01 00:00:00
abstract::Cartilage defects have limited capacity for repair and are often replaced by fibrocartilage with inferior mechanical properties. To overcome the limitations of artificial joint replacement, high-throughput screens (HTS) could be developed to identify molecules that stimulate differentiation and/or proliferation of art...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2010.08.007
更新日期:2011-01-01 00:00:00
abstract::Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, i...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.03.001
更新日期:2014-05-01 00:00:00
abstract::Skin fibroblasts were obtained from four patients with Williams-Beuren syndrome (WBS) carrying the typical 1.5 Mb or 1.8 Mb deletion at the 7q11.23 genomic region. Induced pluripotent stem cells (iPSCs) were generated by retroviral infection of fibroblasts with polycystronic vectors. The generated iPSC clones ESi059A,...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102087
更新日期:2020-12-01 00:00:00
abstract::FOS is component of the AP-1 complex and has been reported to be involved in many cellular functions, including cell proliferation, differentiation, survival, angiogenesis, hematopoiesis and cancer progress. To further understand the exact role of FOS in these processes, here we created two FOS knockout human embryoni...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101479
更新日期:2019-08-01 00:00:00
abstract::Stem cell differentiation is a complex biological event. Our understanding of this process is partly hampered by the co-existence of different cell subpopulations within a given population, which are characterized by different gene expression states driven by different underlying transcriptional regulatory networks (T...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.08.006
更新日期:2015-09-01 00:00:00
abstract::In order to evaluate the functional significance of palmitoylation during multi-potent neural stem/progenitor cell proliferation and differentiation, retinoic acid-induced P19 cells were used in this study as a model system. Cell behaviour was monitored in the presence of the protein palmitoylation inhibitor 2-bromopa...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.12.010
更新日期:2014-03-01 00:00:00
abstract::Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprog...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.03.022
更新日期:2017-05-01 00:00:00
abstract::Glycogen debranching enzyme (GDE) plays a critical role in glycogenolysis. Mutations in the GDE gene are associated with a metabolic disease known as glycogen storage disease type III (GSDIII). We generated a mutant GDE human embryonic stem cell line, WAe001-A-14, using the CRISPR/Cas9 editing system. This cell line c...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.12.009
更新日期:2018-03-01 00:00:00
abstract::Derivation of induced pluripotent stem (iPS) cells is mainly an epigenetic reprogramming process. It is still quite controversial how genomic imprinting is reprogrammed in iPS cells. Thus, we derived multiple iPS clones from genetically identical mouse somatic cells. We found that parentally inherited imprint was vari...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.05.011
更新日期:2013-09-01 00:00:00
abstract::Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting the osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent a valid therapeutic substitute. The aim of this study was to investigate the e...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101463
更新日期:2019-07-01 00:00:00
abstract::Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102069
更新日期:2020-12-01 00:00:00
abstract::Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.01.034
更新日期:2018-04-01 00:00:00
abstract::Autosomal recessive Stargardt disease is the most common cause of inherited retinal disease. In this report, we describe the generation and characterization of two human induced pluripotent stem cell (iPSC) lines from a patient with compound heterozygous mutations in the ABCA4 gene (c.[768G>T];[6079C>T]). Patient derm...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101947
更新日期:2020-10-01 00:00:00
abstract::CHCHD2 mutation has been reported as a potential cause of a rare form of familial Parkinson's disease. Recently, a novel CHCHD2 mutation was identified in a family with Parkinson's disease. The dermal fibroblasts of the patient were obtained and successfully transformed into induced pluripotent stem cells(iPSCs), empl...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.08.011
更新日期:2018-10-01 00:00:00
abstract::Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG-repeat expanding mutation in ATXN3. We generated induced pluripotent stem cells (iPSCs) from a SCA3 patient by electroporation of dermal fibroblasts with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 an...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.02.040
更新日期:2016-05-01 00:00:00
abstract::The future application of human embryonic stem cells (hESC) for therapeutic approaches requires the development of xeno-free culture conditions to prevent the potential transmission of animal pathogens or xenobiotic substances to hESC. An important component of the majority of hESC culture systems developed is the req...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2010.08.002
更新日期:2011-01-01 00:00:00
abstract::Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid bod...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.02.007
更新日期:2017-04-01 00:00:00
abstract::Over-expression of the early neural inducer, Noggin, in nestin positive subventricular zone (SVZ), neural stem cells (NSC) promotes proliferation and neuronal differentiation of neural progenitors and inhibits the expression of a CNS-enriched microRNA-410 (miR-410) (Morell et al., 2015). When expressed in neurospheres...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.07.003
更新日期:2016-09-01 00:00:00
abstract::Gastrointestinal (GI) homeostasis requires the action of multiple pathways. There is some controversy regarding whether small intestine (SI) Paneth cells (PCs) play a central role in orchestrating crypt architecture and their relationship with Lgr5+ve stem cells. Nevertheless, we previously showed that germline CSF-1 ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2012.12.001
更新日期:2013-03-01 00:00:00