当前位置： SCI文献检索 > Stem Cell Research期刊下所有文献 > One-step generation of murine embryonic stem cell-derived mesoderm progenitors and chondrocytes in a serum-free monolayer differentiation system.

One-step generation of murine embryonic stem cell-derived mesoderm progenitors and chondrocytes in a serum-free monolayer differentiation system.

Abstract：

:Cartilage defects have limited capacity for repair and are often replaced by fibrocartilage with inferior mechanical properties. To overcome the limitations of artificial joint replacement, high-throughput screens (HTS) could be developed to identify molecules that stimulate differentiation and/or proliferation of articular cartilage for drug therapy or tissue engineering. Currently embryonic stem cells (ESCs) can differentiate into articular cartilage by forming aggregates (embryoid body (EB), pellet, micromass), which are difficult to image. We present a novel, single-step method of generating murine ESC-derived chondrocytes in monolayer cultures under chemically defined conditions. Mesoderm induction was achieved in cultures supplemented with BMP4, activin A, or Wnt3a. Prolonged culture with sustained activin A, TGFβ3, or BMP4 supplementation led to robust chondrogenic induction. A short pulse of activin A or BMP4 also induced chondrogenesis efficiently while Wnt3a acted as a later inducer. Long-term supplementation with activin A or with activin A followed by TGFβ3 promoted articular cartilage formation. Thus, we devised a serum-free (SF) culture system to generate ESC-derived chondrocytes without the establishment of 3D cultures or the aid of cell sorting. Cultures were governed by the same signaling pathways as 3D ESC differentiation systems and limb bud mesenchyme or articular cartilage explant cultures.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Waese EY,Stanford WL

doi

10.1016/j.scr.2010.08.007

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

34-49

issue

1

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(10)00099-1

journal_volume

6

pub_type

杂志文章

相关文献

Stem Cell Research文献大全
  • Generation of the human induced pluripotent stem cell (hiPSC) line PSMi003-A from a patient affected by an autosomal recessive form of Long QT Syndrome type 1.

    abstract：:We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a 51years old female patient homozygous for the mutation c.535 G>A p.G179S on the KCNQ1 gene, causing a severe form of autosomal recessive Long QT Syndrome type 1 (AR-LQT1), not associated with deafness. The hiPSCs, generated using f...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.04.003

    authors： Mura M,Ginevrino M,Zappatore R,Pisano F,Boni M,Castelletti S,Crotti L,Valente EM,Schwartz PJ,Gnecchi M

    更新日期：2018-05-01 00:00:00

  • Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene.

    abstract：:The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurg...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.01.004

    authors： Miere C,Hewitson H,Devito L,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

    更新日期：2016-03-01 00:00:00

  • Generation of an induced pluripotent stem cell line, CSSi011-A (6534), from an Amyotrophic lateral sclerosis patient with heterozygous L145F mutation in SOD1 gene.

    abstract：:Among the known causative genes of familial ALS, SOD1mutation is one of the most common. It encodes for the ubiquitous detoxifying copper/zinc binding SOD1 enzyme, whose mutations selectively cause motor neuron death, although the mechanisms are not as yet clear. What is known is that mutant-mediated toxicity is not c...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101924

    authors： D'Anzi A,Altieri F,Perciballi E,Ferrari D,Bernardini L,Goldoni M,Mazzini L,De Marchi F,Di Pierro A,D'Alfonso S,Gelati M,Vescovi AL,Rosati J

    更新日期：2020-07-25 00:00:00

  • Cardiac regeneration based on mechanisms of cardiomyocyte proliferation and differentiation.

    abstract：:Cardiomyocyte proliferation and progenitor differentiation are endogenous mechanisms of myocardial development. Cardiomyocytes continue to proliferate in mammals for part of post-natal development. In adult mammals under homeostatic conditions, cardiomyocytes proliferate at an extremely low rate. Because the mechanism...

    journal_title：Stem cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.scr.2014.09.003

    authors： Senyo SE,Lee RT,Kühn B

    更新日期：2014-11-01 00:00:00

  • Characterization of an induced pluripotent stem cell line (IMBPASi001-A) derived from fibroblasts of a patient affected by Wolfram Syndrome.

    abstract：:Wolfram Syndrome is a rare, autosomal recessive genetic disorder with clinical symptoms appearing in early childhood. Here, we report a generation of iPSCs from fibroblasts of a patient affected by this disease. Induced pluripotent cells obtained with the application of integration-free episomal vectors display a norm...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101858

    authors： Grzela DP,Marciniak B,Pulaski L

    更新日期：2020-07-01 00:00:00

  • Establishment of induced pluripotent stem cell line CSUASOi003- A from an autosomal recessive retinitis pigmentosa patient carrying compound heterozygous mutations in CRB1 gene.

    abstract：:Crumbs homologue 1 (CRB1) mutations have been found in retinitis pigmentosa (RP) patients lead to severe retinal dystrophies. The human induced pluripotent stem (iPS) cell line CSUASOi003-A derived from peripheral blood mononuclear cells (PBMCs) of a patient carrying two heterozygous mutations (2249G>A p.G750D and c.2...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101742

    authors： Zhou Y,Ding C,Xia S,Jing Y,Mao S,Liu J,Chen J,Chan HF,Tang S,Chen J

    更新日期：2020-04-01 00:00:00

  • Reporter gene-engineering of human induced pluripotent stem cells during differentiation renders in vivo traceable hepatocyte-like cells accessible.

    abstract：:Primary hepatocyte transplantation (HTx) is a safe cell therapy for patients with liver disease, but wider application is circumvented by poor cell engraftment due to limitations in hepatocyte quality and transplantation strategies. Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (hiPSC)...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101599

    authors： Ashmore-Harris C,Blackford SJ,Grimsdell B,Kurtys E,Glatz MC,Rashid TS,Fruhwirth GO

    更新日期：2019-12-01 00:00:00

  • Establishment of human embryonic stem cell WAe009-A-48 carrying a long QT syndrome mutation in SCN5A.

    abstract：:The long QT syndrome type 3 (LQT3) is currently the 3rd most prevalent of the 15 known types of LQT syndrome. Cardiac events in LQT3 are less frequent than LQT1 and LQT2, but more likely to be fatal. LQT3 is caused by mutation in gene SCN5A, which codes for the Nav1.5 Na+ channel. Herein, we have generated a human emb...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2021.102194

    authors： Yang X,Wu F,Zhong J,Li F

    更新日期：2021-01-25 00:00:00

  • Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD).

    abstract：:An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency marker...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.11.008

    authors： Wang Y,Zhang J,Lei Y,Zhao J

    更新日期：2018-12-01 00:00:00

  • Generation of functional podocytes from human induced pluripotent stem cells.

    abstract：:Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into in...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.06.001

    authors： Ciampi O,Iacone R,Longaretti L,Benedetti V,Graf M,Magnone MC,Patsch C,Xinaris C,Remuzzi G,Benigni A,Tomasoni S

    更新日期：2016-07-01 00:00:00

  • Bone marrow cells play only a very minor role in chronic liver regeneration induced by a choline-deficient, ethionine-supplemented diet.

    abstract：:Liver progenitor (oval) cells have enormous potential in the treatment of patients with liver disease using a cell therapy approach, but their use is limited by their scarcity and the number of donor livers from which they can be derived. Bone marrow may be a suitable source. Previously the derivation of oval cells fr...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2008.05.004

    authors： Tonkin JN,Knight B,Curtis D,Abraham LJ,Yeoh GC

    更新日期：2008-09-01 00:00:00

  • Ocular surface ectoderm instigated by WNT inhibition and BMP4.

    abstract：:We sought to elucidate how and when the ocular surface ectoderm commits to its differentiation into the corneal epithelium in eye development from human induced pluripotent stem cells (hiPSCs) under the influence of WNT signaling and the actions of BMP4. These signals are key drivers ocular surface ectodermal cell fat...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101868

    authors： Kobayashi Y,Hayashi R,Shibata S,Quantock AJ,Nishida K

    更新日期：2020-07-01 00:00:00

  • Repair of acute liver damage with immune evasive hESC derived hepato-blasts.

    abstract：:Human embryonic stem cells (hESCs) can undergo unlimited self-renewal and differentiate into hepatic cells, including expandable hepato-blasts (HBs) and hepatocyte-like cells (HLCs) in vitro. Therefore, hESC-derived HBs have the potential to become a renewable cell source for cell therapy of serious liver damage. Howe...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102010

    authors： Liu J,Pan T,Chen Y,Liu Y,Yang F,Chen Q,Abbas N,Zhong M,Zhang Q,Xu Y,Li YX

    更新日期：2020-12-01 00:00:00

  • Establishment of an induced pluripotent stem cell line (FDEENTi002-A) from a patient with Best's disease carrying c.888C > A mutation in BEST1 gene.

    abstract：:Best's disease (BD) is an inherited retinal degenerative disease caused by mutations in BEST1 gene. A human induced pluripotent stem cell (iPSC) line has been generated with integration-free Sendai virus method from peripheral blood mononuclear cells (PBMCs) of a BD patient carrying c.888C > A mutation in BEST1 gene. ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101459

    authors： Bai X,Yang XJ,Chen L

    更新日期：2019-07-01 00:00:00

  • Generation of 2 induced pluripotent stem cell lines derived from patients with Parkinson's disease carrying LRRK2 G2385R variant.

    abstract：:Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.01.034

    authors： Cheng YC,Huang CY,Ho MC,Hsu YH,Syu SH,Lu HE,Lin HI,Lin CH,Hsieh PCH

    更新日期：2018-04-01 00:00:00

  • Generation of human iPSC line GRX-MCiPS4F-A2 from adult peripheral blood mononuclear cells (PBMCs) with Spanish genetic background.

    abstract：:We have generated iPSCs from peripheral blood mononuclear cells (PBMCs) of a healthy man using heat sensitive and non-integrative Sendai virus containing Sox2, Oct3/4, c-Myc and Klf4. Human GRX-MCiPS4F-A2 cell line was established and characterized through this study. ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.07.002

    authors： Cabrera S,Ji AR,Frejo L,Ramos-Mejia V,Romero T,Real P,Lopez-Escamez JA

    更新日期：2015-09-01 00:00:00

  • Derivation, characterization, and gene expression profile of two new human ES cell lines from India.

    abstract：:Human embryonic stem cells (hESCs) offer new avenues for studying human development and disease progression in addition to their tremendous potential toward development of cell-replacement therapies for various cellular disorders. We have earlier reported the derivation and characterization of Relicell(®) hES1, the fi...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2010.07.001

    authors： Mandal A,Bhowmik S,Patki A,Viswanathan C,Majumdar AS

    更新日期：2010-11-01 00:00:00

  • Generation of induced pluripotent stem cell line (ZZUi004-A) from urine sample of a patient with spinocerebellar ataxia type 3.

    abstract：:Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the region of the ATXN3 gene. The main feature of SCA3 is progressive ataxia, which affects balance, gait, and speech. Urine cells (UCs) of a SCA3 patient were successfully translated to induced...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.01.027

    authors： Wang Y,Shi C,Wang Z,Sun H,Yang Z,Zhang F,Liu Y,Liu H,Jiang C,Zhang S,Xu Y,Wen X

    更新日期：2018-04-01 00:00:00

  • Generation of GADD45A gene knockout human embryonic stem cell line using CRISPR/Cas9.

    abstract：:GADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been me...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102090

    authors： Li J,Li Y,Zhan XY,Ran X,Tse HF,Dang S,Nie Y,Huang K

    更新日期：2020-12-01 00:00:00

  • The cytokine-mediated crosstalk between primary human acute myeloid cells and mesenchymal stem cells alters the local cytokine network and the global gene expression profile of the mesenchymal cells.

    abstract：:Interactions between acute myeloid leukemia (AML) blasts and neighboring stromal cells are important for disease development and chemosensitivity. However, the molecular mechanisms involved in the cytokine-mediated crosstalk between mesenchymal stem cells (MSCs) and AML cells are largely unknown. Leukemic cells derive...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.09.008

    authors： Reikvam H,Brenner AK,Hagen KM,Liseth K,Skrede S,Hatfield KJ,Bruserud Ø

    更新日期：2015-11-01 00:00:00

  • Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease.

    abstract：:Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.12.026

    authors： Chumarina M,Azevedo C,Bigarreau J,Vignon C,Kim KS,Li JY,Roybon L

    更新日期：2017-03-01 00:00:00

  • Stage-specific roles of FGF2 signaling in human neural development.

    abstract：:This study elucidated the stage-specific roles of FGF2 signaling during neural development using in-vitro human embryonic stem cell-based developmental modeling. We found that the dysregulation of FGF2 signaling prior to the onset of neural induction resulted in the malformation of neural rosettes (a neural tube-like ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.08.012

    authors： Grabiec M,Hříbková H,Vařecha M,Střítecká D,Hampl A,Dvořák P,Sun YM

    更新日期：2016-09-01 00:00:00

  • Constitutive transgene expression of Stem Cell Antigen-1 in the hair follicle alters the sensitivity to tumor formation and progression.

    abstract：:The cell surface protein Stem Cell Antigen-1 (Sca-1) marks stem or progenitor cells in several murine tissues and is normally upregulated during cancer development. Although the specific function of Sca-1 remains unknown, Sca-1 seems to play a role in proliferation, differentiation and cell migration in a number of ti...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2017.07.002

    authors： Christensen R,Owens DM,Füchtbauer AC,Gunnarsson A,Ramsing M,Füchtbauer EM,Jensen UB

    更新日期：2017-08-01 00:00:00

  • Feeder-independent derivation of induced-pluripotent stem cells from peripheral blood endothelial progenitor cells.

    abstract：:Induced-pluripotent stem cells (iPSCs) are a potential alternative cell source in regenerative medicine, which includes the use of differentiated iPSCs for cell therapies to treat coronary artery and/or peripheral arterial diseases. Late-outgrowth endothelial progenitor cells (late-EPCs) are a unique primary cell pres...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2012.11.006

    authors： Chang WY,Lavoie JR,Kwon SY,Chen Z,Manias JL,Behbahani J,Ling V,Kandel RA,Stewart DJ,Stanford WL

    更新日期：2013-03-01 00:00:00

  • Generation of the human induced pluripotent stem cell line (SHAMUi001-A) carrying the heterozygous c.-128G>T mutation in the 5'-UTR of the ANKRD26 gene.

    abstract：:Thrombocytopenia 2 (THC2) is a major type of inherited thrombocytopenia caused by the persistent ANKRD26 expression during the late stage of megakaryocytopoiesis. For the first time, we generated a human induced pluripotent stem cell (hiPSC) line SHAMUi001-A from the bone marrow hematopoietic progenitor cells of a THC...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102002

    authors： Tan C,Dai L,Yang W,Li F,Wang L,Xiao Y,Wang X,Zhang Y,Wang Y,Zeng C,Xiang Z,Zhang X,Zhang W,Ran Q,Chen M,Li Z,Chen L

    更新日期：2020-10-01 00:00:00

  • Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation.

    abstract：:Hypertrophic cardiomyopathy is an inherited cardiomyopathy with a prevalence of up to 1 in 200, which can result in significant morbidity and mortality. An iPSC line was generated from peripheral blood mononuclear cells obtained from the whole blood of a 58-year-old male with hypertrophic cardiomyopathy who carries th...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.10.009

    authors： Holliday M,Ross SB,Lim S,Semsarian C

    更新日期：2018-12-01 00:00:00

  • Generation of non-transgenic iPS cells from human cord blood CD34+ cells under animal component-free conditions.

    abstract：:Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprog...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2017.03.022

    authors： Haase A,Göhring G,Martin U

    更新日期：2017-05-01 00:00:00

  • A novel variant of Oct3/4 gene in mouse embryonic stem cells.

    abstract：:OCT4 is a highly conserved gene and plays an important role during early embryonic development and differentiation. Similar to human OCT4, mouse Oct4 gene generates variants. Oct4A is a master regulator of self-renewal in pluripotent stem cells. In this study, we have identified a novel Oct4 spliced variant, designate...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2012.04.004

    authors： Guo CL,Liu L,Jia YD,Zhao XY,Zhou Q,Wang L

    更新日期：2012-09-01 00:00:00

  • Cell division in the cerebral cortex of adult rats after photothrombotic ring stroke.

    abstract：:Neurogenesis has been shown to occur in the cerebral cortex in adult rats after ischemic stroke. The origin of the newborn neurons is largely unknown. This study aimed to explore cell division in the poststroke penumbral cortex. Adult male Wistar rats were subjected to photothrombotic ring stroke. After repeated deliv...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2008.07.003

    authors： Gu W,Brännström T,Rosqvist R,Wester P

    更新日期：2009-01-01 00:00:00

  • Impact of transient down-regulation of DREAM in human embryonic stem cell pluripotency: The role of DREAM in the maintenance of hESCs.

    abstract：:Little is known about the functions of downstream regulatory element antagonist modulator (DREAM) in embryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca(2+)-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are inv...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.03.001

    authors： Fontán-Lozano A,Capilla-Gonzalez V,Aguilera Y,Mellado N,Carrión AM,Soria B,Hmadcha A

    更新日期：2016-05-01 00:00:00