Abstract：
:Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 60-year-old female diagnosed with sporadic amyotrophic lateral sclerosis (sALS). The iPSCs shared the same karyotype with the parent PBMCs, expressed pluripotency stem cell markers, and demonstrated trilineage differentiation potential. This cell line could serve as an ideal model to investigate the mechanisms underlying amyotrophic lateral sclerosis (ALS).
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Feng B,Ma J,Amponsah AE,Guo R,Kong D,He J,Jiang Y,Zhang W,Zhang Z,Song Y,Shen S,O'Brien T,Cui Hdoi
10.1016/j.scr.2020.101841subject
Has Abstractpub_date
2020-05-01 00:00:00pages
101841eissn
1873-5061issn
1876-7753pii
S1873-5061(20)30142-2journal_volume
45pub_type
杂志文章abstract：:As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated wit...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.101360
更新日期：2019-01-01 00:00:00
abstract：:Both BMP and Wnt signaling control stem cells in bulge/dermal papilla, intestinal crypt, and bone marrow. To explore their roles in the limbal niche, which govern corneal epithelial homeostasis, we established an in vitro model of sphere growth by reunion between single limbal epithelial progenitor cells (LEPCs) and a...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.01.003
更新日期：2014-03-01 00:00:00
abstract：:Characterisation of mouse pluripotent stem cells has revealed two distinct pluripotent states, naive and primed, that maintain characteristics of the pre and post implanted epiblast respectively. Recent studies have developed several culture systems that seek to recapitulate the naive phenomenon in human pluripotent s...
journal_title：Stem cell research
pub_type： 杂志文章,评审
doi：10.1016/j.scr.2017.11.017
更新日期：2018-01-01 00:00:00
abstract：:The human iPSC cell line, CARS-FiPS4F1 (ESi064-A), derived from dermal fibroblast from the apparently healthy carrier of the mutation of the gene SACSIN, was generated by non-integrative reprogramming technology using OCT3/4, SOX2, CMYC and KLF4 reprogramming factors. The pluripotency was assessed by immunocytochemist...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.10.016
更新日期：2018-12-01 00:00:00
abstract：:Our work aimed at evaluating the role of adipose stem cells (ASC) on chondrocytes from osteoarthritic (OA) patients and identifying the mediators involved. We used primary chondrocytes, ASCs from different sources and bone marrow mesenchymal stromal cells (MSC) from OA donors. ASCs or MSCs were co-cultured with chondr...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2013.05.008
更新日期：2013-09-01 00:00:00
abstract：:Oligodendrocyte precursor cells (OPCs) differentiation from multipotent neural stem cells (NSCs) into mature oligodendrocytes is driven by thyroid hormone and mediated by thyroid hormone receptors (TRs). We show that several nuclear receptors display strong changes in expression levels between fetal and adult NSCs, wi...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101443
更新日期：2019-05-01 00:00:00
abstract：:Transplantation of primary hepatocytes has been used in treatments for various liver pathologies and end-stage liver disease. However, shortage of donor tissue and the inability of hepatocyte proliferation in vitro have lead to alternative methods such as stem cell-derived hepatocyte-like cells (HLCs). Mesenchymal str...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101471
更新日期：2019-07-01 00:00:00
abstract：:Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressin...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101506
更新日期：2019-08-01 00:00:00
abstract：:Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunesuppressive effects. We cotransplan...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2013.10.001
更新日期：2014-01-01 00:00:00
abstract：:Two iPSC clones, NCCSi008-A and NCCSi008-B, were generated from a healthy male individual of Indian origin by reprogramming his CD4+ T cells with an integration free Sendai viral vector. The established iPSC clones showed high alkaline phosphatase (ALP) activity, expression of pluripotency markers, a normal male karyo...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.101765
更新日期：2020-05-01 00:00:00
abstract：:Skin fibroblasts were obtained from four patients with Williams-Beuren syndrome (WBS) carrying the typical 1.5 Mb or 1.8 Mb deletion at the 7q11.23 genomic region. Induced pluripotent stem cells (iPSCs) were generated by retroviral infection of fibroblasts with polycystronic vectors. The generated iPSC clones ESi059A,...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102087
更新日期：2020-12-01 00:00:00
abstract：:Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.09.001
更新日期：2012-03-01 00:00:00
abstract：:Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was con...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.12.002
更新日期：2017-01-01 00:00:00
abstract：:Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, s...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101590
更新日期：2019-12-01 00:00:00
abstract：:Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into in...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.06.001
更新日期：2016-07-01 00:00:00
abstract：:Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial disease caused by homoplasmic point mutations in complex I subunit genes of mitochondrial DNA. In this report, we generated an induced pluripotent stem cell (iPSCs) line, TVGH-iPSC-010-09, from the peripheral blood mononuclear cells of ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.01.029
更新日期：2018-04-01 00:00:00
abstract：:Dominant deafness-onychodystrophy (DDOD) syndrome is a rare, autosomal dominant inherited disorder with no concrete therapies in human. We previously identified c.1516 C > T (p.Arg506*) in ATP6V1B2 as cause of DDOD syndrome, accounting for all cases of this genetic disorder. The induced pluripotent stem cell (iPSC) li...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.101986
更新日期：2020-10-01 00:00:00
abstract：:Transgene free UNIPDi002-A-human induced pluripotent stem cell (hiPSC) line was generated by Sendai Virus Vectors reprogramming from human oral mucosal epithelial stem cells (hOMESCs) of a patient affected by ectrodactyly-ectodermal dysplasia-clefting (EEC)-syndrome, carrying a mutation in exon 8 of the TP63 gene (R30...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.02.006
更新日期：2018-04-01 00:00:00
abstract：:The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Plurip...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.12.005
更新日期：2017-01-01 00:00:00
abstract：:Pathological myopia (PM) is a retinal degenerative disease with an increasing prevalence in Asia. The peripheral blood mononuclear cells (PBMCs) from a patient with PM were successfully reprogrammed to induced pluripotent stem cells (iPSCs) using integration-free method, Sendai viral (SeV) vectors expressing OCT4, SOX...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.101369
更新日期：2019-01-01 00:00:00
abstract：:The niche concept of stem cell biology proposes a functional unit between the precursor cells and their local microenvironment, to which several cell types might contribute by cell-cell contacts, extracellular matrix, and humoral factors. We here established three co-culture models (with cell types separated by membra...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.09.010
更新日期：2015-11-01 00:00:00
abstract：:Liver progenitor (oval) cells have enormous potential in the treatment of patients with liver disease using a cell therapy approach, but their use is limited by their scarcity and the number of donor livers from which they can be derived. Bone marrow may be a suitable source. Previously the derivation of oval cells fr...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2008.05.004
更新日期：2008-09-01 00:00:00
abstract：:Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprog...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.03.022
更新日期：2017-05-01 00:00:00
abstract：:The KCL017 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676+3A>T). The ICM was isolated using laser microsurgery and plated on γ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.01.013
更新日期：2016-03-01 00:00:00
abstract：:Primary hyperoxaluria type 1 (PH1) is a rare autosomal recessive disorder of the liver metabolism due to functional deficiency of the peroxisomal enzyme alanine:glyoxylate aminotransferase (AGT). AGT deficiency results in overproduction of oxalate which complexes with calcium to form insoluble calcium-oxalate salts in...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101467
更新日期：2019-07-01 00:00:00
abstract：:Endothelial progenitor cells (EPCs) may contribute to neurovascular repair after stroke and neurodegeneration. A key step in this process should involve adhesive interactions between EPCs and the targeted cerebral endothelium. Here, we tested the hypothesis that reactive astrocytes may play a critical role in enhancin...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2013.12.008
更新日期：2014-03-01 00:00:00
abstract：:The human embryonic stem cell line RCe019-A (RC-15) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a cleavage st...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.04.003
更新日期：2016-05-01 00:00:00
abstract：:We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 10years old patient with primary congenital glaucoma (PCG). The cells were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system and shown to have full differentiation potential. ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.04.004
更新日期：2018-05-01 00:00:00
abstract：:A skin biopsy was obtained from a 25-year-old female patient with autosomal recessive Alport syndrome (ARAS) with the homozygous COL4A3 mutation c.345delG, p.(P166Lfs*37). Dermal fibroblasts were derived and reprogrammed by nucleofection with episomal plasmids carrying OCT3/4, SOX2, KLF4 LIN28, L-MYC and p53shRNA. The...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.08.021
更新日期：2017-12-01 00:00:00
abstract：:The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.12.034
更新日期：2016-01-01 00:00:00
