当前位置： SCI文献检索 > Stem Cell Research期刊下所有文献 > Generation of non-transgenic iPS cells from human cord blood CD34+ cells under animal component-free conditions.

Generation of non-transgenic iPS cells from human cord blood CD34+ cells under animal component-free conditions.

Abstract：

:Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34+ cell isolation, cultivation, reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Haase A,Göhring G,Martin U

doi

10.1016/j.scr.2017.03.022

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

71-73

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(17)30066-1

journal_volume

21

pub_type

杂志文章

相关文献

Stem Cell Research文献大全
  • Generation of three iPSC lines (IAIi002, IAIi004, IAIi003) from Rubinstein-Taybi syndrome 1 patients carrying CREBBP non sense c.4435G>T, p.(Gly1479*) and c.3474G>A, p.(Trp1158*) and missense c.4627G>T, p.(Asp1543Tyr) mutations.

    abstract：:Rubinstein-Taybi syndrome (RSTS) is a neurodevelopmental disorder characterized by growth retardation, skeletal anomalies and intellectual disability, caused by heterozygous mutations in either CREBBP (RSTS1) or EP300 (RSTS2) genes. We characterized 3 iPSC lines generated by Sendai from blood of RSTS1 patients with un...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101553

    authors： Alari V,Russo S,Rovina D,Garzo M,Crippa M,Calzari L,Scalera C,Concolino D,Castiglioni E,Giardino D,Prosperi E,Finelli P,Gervasini C,Gowran A,Larizza L

    更新日期：2019-10-01 00:00:00

  • Generation of an iPSC line of a patient with Angelman syndrome due to an imprinting defect.

    abstract：:Angelman syndrome (AS) is a neurodevelopmental disorder with leading symptoms of happy demeanor, intellectual disability, ataxia and seizures. AS can be caused by genetic and epigenetic aberrations, resulting in the absence of functional UBE3A protein in the brain. UBE3A is an imprinted gene, which is, in neurons of t...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.09.015

    authors： Neureiter A,Brändl B,Hiber M,Tandon R,Müller FJ,Steenpass L

    更新日期：2018-12-01 00:00:00

  • Generation of induced pluripotent stem cells-derived hepatocyte-like cells for ex vivo gene therapy of primary hyperoxaluria type 1.

    abstract：:Primary hyperoxaluria type 1 (PH1) is a rare autosomal recessive disorder of the liver metabolism due to functional deficiency of the peroxisomal enzyme alanine:glyoxylate aminotransferase (AGT). AGT deficiency results in overproduction of oxalate which complexes with calcium to form insoluble calcium-oxalate salts in...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101467

    authors： Estève J,Blouin JM,Lalanne M,Azzi-Martin L,Dubus P,Bidet A,Harambat J,Llanas B,Moranvillier I,Bedel A,Moreau-Gaudry F,Richard E

    更新日期：2019-07-01 00:00:00

  • Human embryonic fibroblasts support single cell enzymatic expansion of human embryonic stem cells in xeno-free cultures.

    abstract：:The future application of human embryonic stem cells (hESC) for therapeutic approaches requires the development of xeno-free culture conditions to prevent the potential transmission of animal pathogens or xenobiotic substances to hESC. An important component of the majority of hESC culture systems developed is the req...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2010.08.002

    authors： Kibschull M,Mileikovsky M,Michael IP,Lye SJ,Nagy A

    更新日期：2011-01-01 00:00:00

  • Cardiac regeneration based on mechanisms of cardiomyocyte proliferation and differentiation.

    abstract：:Cardiomyocyte proliferation and progenitor differentiation are endogenous mechanisms of myocardial development. Cardiomyocytes continue to proliferate in mammals for part of post-natal development. In adult mammals under homeostatic conditions, cardiomyocytes proliferate at an extremely low rate. Because the mechanism...

    journal_title：Stem cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.scr.2014.09.003

    authors： Senyo SE,Lee RT,Kühn B

    更新日期：2014-11-01 00:00:00

  • TET2 haploinsufficiency alters reprogramming into induced pluripotent stem cells.

    abstract：:The discovery of the Ten-Eleven Translocation (TET) protein family was initiated by the identification of the MLL partner TET1, and of mutations in the TET2 gene in hematological malignancies including myeloproliferative neoplasms (MPN). TET1, 2 and 3 proteins hydroxylate 5-methylcytosine (5-mC) into 5-hydroxymethylcy...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101755

    authors： Secardin L,Limia CEG,di Stefano A,Bonamino MH,Saliba J,Kataoka K,Rehen SK,Raslova H,Marty C,Ogawa S,Vainchenker W,Monte-Mor BDCR,Plo I

    更新日期：2020-04-01 00:00:00

  • L-WRN conditioned medium for gastrointestinal epithelial stem cell culture shows replicable batch-to-batch activity levels across multiple research teams.

    abstract：:Conditioned medium (CM) derived from engineered cells often facilitates the cost-effective culture of a variety of stem cells. Growing emphasis on the importance of rigor and reproducibility in lab-based science requires development of best practices approaches, including quality control procedures for the assessment ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101430

    authors： VanDussen KL,Sonnek NM,Stappenbeck TS

    更新日期：2019-05-01 00:00:00

  • Derivation of the clinical grade human embryonic stem cell line RCe019-A (RC-15).

    abstract：:The human embryonic stem cell line RCe019-A (RC-15) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a cleavage st...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.04.003

    authors： De Sousa PA,Tye BJ,Bruce K,Dand P,Russell G,Collins DM,Greenshields A,McDonald K,Bradburn H,Laurie A,Downie JM,Bateman M,Courtney A

    更新日期：2016-05-01 00:00:00

  • Generation of iPSC lines from primary human amniotic fluid cells.

    abstract：:By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human emb...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.11.003

    authors： Drews K,Matz P,Adjaye J

    更新日期：2015-11-01 00:00:00

  • Impact of transient down-regulation of DREAM in human embryonic stem cell pluripotency: The role of DREAM in the maintenance of hESCs.

    abstract：:Little is known about the functions of downstream regulatory element antagonist modulator (DREAM) in embryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca(2+)-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are inv...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.03.001

    authors： Fontán-Lozano A,Capilla-Gonzalez V,Aguilera Y,Mellado N,Carrión AM,Soria B,Hmadcha A

    更新日期：2016-05-01 00:00:00

  • Stage-specific roles of FGF2 signaling in human neural development.

    abstract：:This study elucidated the stage-specific roles of FGF2 signaling during neural development using in-vitro human embryonic stem cell-based developmental modeling. We found that the dysregulation of FGF2 signaling prior to the onset of neural induction resulted in the malformation of neural rosettes (a neural tube-like ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.08.012

    authors： Grabiec M,Hříbková H,Vařecha M,Střítecká D,Hampl A,Dvořák P,Sun YM

    更新日期：2016-09-01 00:00:00

  • Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system.

    abstract：:Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Amniotic fluid (AF) cells are easy to obtain from routinely scheduled procedures for prenatal diagnosis and iPS cells have been generated from human AF. H...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.09.009

    authors： Bertin E,Piccoli M,Franzin C,Nagy A,Mileikovsky M,De Coppi P,Pozzobon M

    更新日期：2015-11-01 00:00:00

  • The CSF-1 receptor fashions the intestinal stem cell niche.

    abstract：:Gastrointestinal (GI) homeostasis requires the action of multiple pathways. There is some controversy regarding whether small intestine (SI) Paneth cells (PCs) play a central role in orchestrating crypt architecture and their relationship with Lgr5+ve stem cells. Nevertheless, we previously showed that germline CSF-1 ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2012.12.001

    authors： Akcora D,Huynh D,Lightowler S,Germann M,Robine S,de May JR,Pollard JW,Stanley ER,Malaterre J,Ramsay RG

    更新日期：2013-03-01 00:00:00

  • GABP is necessary for stem/progenitor cell maintenance and myeloid differentiation in human hematopoiesis and chronic myeloid leukemia.

    abstract：:Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of m...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.04.007

    authors： Manukjan G,Ripperger T,Venturini L,Stadler M,Göhring G,Schambach A,Schlegelberger B,Steinemann D

    更新日期：2016-05-01 00:00:00

  • Establishment of induced pluripotent stem cell line CSUASOi004-A by reprogramming peripheral blood mononuclear cells of a PRPF6-related dominant retinitis pigmentosa patient.

    abstract：:We have established the patient-specific induced pluripotent stem (iPS) cell line CSUASOi004-A by using peripheral blood mononuclear cells (PBMCs) of a retinitis pigmentosa (RP) patient with a PRPF6 gene mutation (c.G2699A:p.R900H). CSUASOi004-A was established by a non-integrative method with four episomal plasmids c...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101793

    authors： Zhou Y,Jing Y,Mao S,Liu J,Cui Z,Wang Y,Chen J,Chan HF,Tang S,Chen J

    更新日期：2020-05-01 00:00:00

  • Eltrombopag, a thrombopoietin receptor agonist, enhances human umbilical cord blood hematopoietic stem/primitive progenitor cell expansion and promotes multi-lineage hematopoiesis.

    abstract：:Umbilical cord blood (UCB) transplantation has emerged as a promising therapy, but it is challenged by scarcity of stem cells. Eltrombopag is a non-peptide, thrombopoietin (TPO) receptor agonist, which selectively activates c-Mpl in humans and chimpanzees. We investigated eltrombopag's effects on human UCB hematopoiet...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2012.05.001

    authors： Sun H,Tsai Y,Nowak I,Liesveld J,Chen Y

    更新日期：2012-09-01 00:00:00

  • Generation of three spinocerebellar ataxia type-12 patients derived induced pluripotent stem cell lines (IGIBi002-A, IGIBi003-A and IGIBi004-A).

    abstract：:Spinocerebellar ataxia type-12 (SCA12) is a neurological disorder caused due to triplet (CAG) repeat expansion in 5' UTR of PPP2R2B. It is one of the most prominent SCA-subtype in Indian population and till date no patient specific models have been described. Human-induced-pluripotent-stem cell (HiPSC) based disease m...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.08.008

    authors： Kumar D,Hussain A,Srivastava AK,Mukerji M,Mukherjee O,Faruq M

    更新日期：2018-08-01 00:00:00

  • Laminin α5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro.

    abstract：:Laminins are one of the major protein groups in the extracellular matrix (ECM) and specific laminin isoforms are crucial for neuronal functions in the central nervous system in vivo. In the present study, we compared recombinant human laminin isoforms (LN211, LN332, LN411, LN511, and LN521) and laminin isoform fragmen...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2017.09.002

    authors： Hyysalo A,Ristola M,Mäkinen ME,Häyrynen S,Nykter M,Narkilahti S

    更新日期：2017-10-01 00:00:00

  • Characterization of an induced pluripotent stem cell line (IMBPASi001-A) derived from fibroblasts of a patient affected by Wolfram Syndrome.

    abstract：:Wolfram Syndrome is a rare, autosomal recessive genetic disorder with clinical symptoms appearing in early childhood. Here, we report a generation of iPSCs from fibroblasts of a patient affected by this disease. Induced pluripotent cells obtained with the application of integration-free episomal vectors display a norm...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101858

    authors： Grzela DP,Marciniak B,Pulaski L

    更新日期：2020-07-01 00:00:00

  • Adipose-derived stromal cell secretome reduces TNFα-induced hypertrophy and catabolic markers in primary human articular chondrocytes.

    abstract：:Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting the osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent a valid therapeutic substitute. The aim of this study was to investigate the e...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101463

    authors： Niada S,Giannasi C,Gomarasca M,Stanco D,Casati S,Brini AT

    更新日期：2019-07-01 00:00:00

  • Adrenomedullin promotes differentiation of oligodendrocyte precursor cells into myelin-basic-protein expressing oligodendrocytes under pathological conditions in vitro.

    abstract：:Oligodendrocytes, which are the main cell type in cerebral white matter, are generated from their precursor cells (oligodendrocyte precursor cells: OPCs). However, the differentiation from OPCs to oligodendrocytes is disturbed under stressed conditions. Therefore, drugs that can improve oligodendrocyte regeneration ma...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.05.001

    authors： Maki T,Takahashi Y,Miyamoto N,Liang AC,Ihara M,Lo EH,Arai K

    更新日期：2015-07-01 00:00:00

  • Generation of a human iPS cell line (CGMH.SLC26A4919-2) from a Pendred syndrome patient carrying SLC26A4 c.919-2A>G splice-site mutation.

    abstract：:SLC26A4 is the second most frequent gene implicated in congenital hearing loss after GJB2 mutations. Here, we report the generation of induced pluripotent stem cells (iPSCs), from a patient who was carrying a homozygous c.919-2A>G variant in the SLC26A4 gene. This is the most common variant of SLC26A4 gene in the Chin...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2019.101524

    authors： Cheng YF,Chan YH,Hu CJ,Lu YC,Saeki T,Hosoya M,Saegusa C,Fujioka M,Okano H,Weng SM,Hsu CJ,Chang KH,Wu CC

    更新日期：2019-10-01 00:00:00

  • Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain.

    abstract：:Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.04.015

    authors： Hallmann AL,Araúzo-Bravo MJ,Zerfass C,Senner V,Ehrlich M,Psathaki OE,Han DW,Tapia N,Zaehres H,Schöler HR,Kuhlmann T,Hargus G

    更新日期：2016-05-01 00:00:00

  • Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system.

    abstract：:Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102069

    authors： Zhang C,Yu Y,Li F,Lan X,Wang L

    更新日期：2020-12-01 00:00:00

  • Cholesterol-derived glucocorticoids control early fate specification in embryonic stem cells.

    abstract：:Aside from its role in cell membrane integrity, cholesterol is a key component in steroid hormone production. The vital functions of steroid hormones such as estrogen, testosterone, glucocorticoids (Gcrts) and mineralocorticoids (Mnrts) in perinatal and adult life are well understood; however, their role during early ...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2015.04.010

    authors： Cabral-Teixeira J,Martinez-Fernandez A,Cai W,Terzic A,Mercola M,Willems E

    更新日期：2015-07-01 00:00:00

  • Generation of functional podocytes from human induced pluripotent stem cells.

    abstract：:Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into in...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2016.06.001

    authors： Ciampi O,Iacone R,Longaretti L,Benedetti V,Graf M,Magnone MC,Patsch C,Xinaris C,Remuzzi G,Benigni A,Tomasoni S

    更新日期：2016-07-01 00:00:00

  • Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults.

    abstract：:We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2018.101380

    authors： Vlahos K,Sourris K,Mayberry R,McDonald P,Bruveris FF,Schiesser JV,Bozaoglu K,Lockhart PJ,Stanley EG,Elefanty AG

    更新日期：2019-01-01 00:00:00

  • Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs.

    abstract：:The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.102120

    authors： Zhou M,Wei R,Jiang Y,Fu J,Liu Y,Yang B,Yu B,Lin Y,Ran X,Lai WH,Chu M,Hu Y,Yang J,Tse HF

    更新日期：2020-12-10 00:00:00

  • Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

    abstract：:In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carr...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101806

    authors： Sawada T,Benjamin KJM,Brandtjen AC,Tietze E,Allen SJ,Paquola ACM,Kleinman JE,Hyde TM,Erwin JA

    更新日期：2020-07-01 00:00:00

  • Ocular surface ectoderm instigated by WNT inhibition and BMP4.

    abstract：:We sought to elucidate how and when the ocular surface ectoderm commits to its differentiation into the corneal epithelium in eye development from human induced pluripotent stem cells (hiPSCs) under the influence of WNT signaling and the actions of BMP4. These signals are key drivers ocular surface ectodermal cell fat...

    journal_title：Stem cell research

    pub_type： 杂志文章

    doi：10.1016/j.scr.2020.101868

    authors： Kobayashi Y,Hayashi R,Shibata S,Quantock AJ,Nishida K

    更新日期：2020-07-01 00:00:00