Abstract：
:Little is known about the functions of downstream regulatory element antagonist modulator (DREAM) in embryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca(2+)-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are involved in maintaining ESC self-renewal and pluripotency. However, a previous knockout study revealed the protective function of DREAM depletion in brain aging degeneration and that aging is accompanied by a progressive decline in stem cells (SCs) function. Interestingly, we found that DREAM is expressed in different cell types, including human ESCs (hESCs), human adipose-derived stromal cells (hASCs), human bone marrow-derived stromal cells (hBMSCs), and human newborn foreskin fibroblasts (hFFs), and that transitory inhibition of DREAM in hESCs reduces their pluripotency, increasing differentiation. We stipulate that these changes are partly mediated by increased CREB transcriptional activity. Overall, our data indicates that DREAM acts in the regulation of hESC pluripotency and could be a target to promote or prevent differentiation in embryonic cells.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Fontán-Lozano A,Capilla-Gonzalez V,Aguilera Y,Mellado N,Carrión AM,Soria B,Hmadcha Adoi
10.1016/j.scr.2016.03.001subject
Has Abstractpub_date
2016-05-01 00:00:00pages
568-78issue
3eissn
1873-5061issn
1876-7753pii
S1873-5061(16)00082-9journal_volume
16pub_type
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journal_title：Stem cell research
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Stem cell research
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journal_title：Stem cell research
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journal_title：Stem cell research
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journal_title：Stem cell research
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pub_type： 杂志文章,评审
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journal_title：Stem cell research
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journal_title：Stem cell research
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abstract：:Derivation of induced pluripotent stem (iPS) cells is mainly an epigenetic reprogramming process. It is still quite controversial how genomic imprinting is reprogrammed in iPS cells. Thus, we derived multiple iPS clones from genetically identical mouse somatic cells. We found that parentally inherited imprint was vari...
journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2013-09-01 00:00:00
abstract：:Gene therapy mediated by bone marrow-derived hematopoietic stem cells (BM-HSC) has been widely used in treating genetic deficiencies in both pre-clinical and clinical settings. Using mitotically inactive cell-targeting lentivirus with separate promoters for our gene of interest (the murine MHC class II (MHCII) chapero...
journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
