Abstract:
:Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of murine hematopoietic and leukemic stem cells. Here, we report the consequences of functional impairment of GABP in human hematopoietic and in leukemic stem/progenitor cells. Ectopic overexpression of a dominant-negative acting GABP mutant led to impaired myeloid differentiation of CD34(+) hematopoietic stem/progenitor cells obtained from healthy donors. Moreover, drastically reduced clonogenic capacity of leukemic stem/progenitor cells isolated from bone marrow aspirates of chronic myeloid leukemia (CML) patients underlines the importance of GABP on stem/progenitor cell maintenance and confirms the relevance of GABP for human myelopoiesis in healthy and diseased states.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Manukjan G,Ripperger T,Venturini L,Stadler M,Göhring G,Schambach A,Schlegelberger B,Steinemann Ddoi
10.1016/j.scr.2016.04.007subject
Has Abstractpub_date
2016-05-01 00:00:00pages
677-81issue
3eissn
1873-5061issn
1876-7753pii
S1873-5061(16)30018-6journal_volume
16pub_type
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