Abstract：
:Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. Here, we investigated whether cell migration could be used to compare young and senescent cells. We used 'electrotaxis' where cells under electric treatment move towards the anode or cathode. Without an electric stimulus, stem cells moved randomly. However, under a direct electric current, the cells moved with directionality. Under stimulation with a direct electric current, early passage stem cells moved towards the anode; when the cells became senescent with increasing passages, the percentage of cells migrating to the anode decreased. These results suggest that the behavior of stem cells under the influence of a direct electric current is also related to their passage number. Therefore, electrotaxis migration analysis can be used to distinguish between young cell and senescent cells.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Hong SH,Lee MH,Koo MA,Seon GM,Park YJ,Kim D,Park JCdoi
10.1016/j.scr.2019.101475subject
Has Abstractpub_date
2019-07-01 00:00:00pages
101475eissn
1873-5061issn
1876-7753pii
S1873-5061(19)30105-9journal_volume
38pub_type
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