Abstract：
:Neuroblasts represent the predominant migrating cell type in the adult mouse brain. There are, however, increasing evidences of migration of other neural precursors. This work aims at identifying in vivo endogenous early neural precursors, different from neuroblasts, able to migrate in response to brain injuries. The monoclonal antibody Nilo1, which unequivocally identifies type B astrocytes and embryonic radial glia, was coupled to magnetic glyconanoparticles (mGNPs). Here we show that Nilo1-mGNPs in combination with magnetic resonance imaging in living mice allowed the in vivo identification of endogenous type B astrocytes at their niche, as well as their migration to the lesion site in response to glioblastoma, demyelination, cryolesion or mechanical injuries. In addition, Nilo1(+) adult radial glia-like structures were identified at the lesion site a few hours after damage. For all damage models used, type B astrocyte migration was fast and orderly. Identification of Nilo1(+) cells surrounding an induced glioblastoma was also possible after intraperitoneal injection of the antibody. This opens up the possibility of an early identification of the initial damage site(s) after brain insults, by the migration of type B astrocytes.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Elvira G,García I,Gallo J,Benito M,Montesinos P,Holgado-Martin E,Ayuso-Sacido A,Penadés S,Desco M,Silva A,Garcia-Sanz JAdoi
10.1016/j.scr.2014.11.006subject
Has Abstractpub_date
2015-01-01 00:00:00pages
114-29issue
1eissn
1873-5061issn
1876-7753pii
S1873-5061(14)00142-1journal_volume
14pub_type
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