Abstract：
:Extracellular signal-regulated kinases (ERKs) have many important functions during embryogenesis. However, their role in embryonic stem (ES) cells is controversial. Previous studies reported that, in contrast to mouse ES cells, human ES cells differentiate if ERK1/2 is inhibited. We reexamined the role of ERK1/2 in human ES cells using a chemically defined culture system and found that when ERK1/2 is blocked with specific chemical inhibitors, neural and mesendodermal differentiation is prevented, but cells become sensitive to BMP-induced differentiation. Inhibition of ERK1/2 significantly reduced the clonogenicity of human ES cells by preventing cell adhesion and survival. When this negative effect was avoided, we were able to maintain human ES cell self-renewal for more than 3months in the presence of ERK1/2 inhibitors in a chemically defined culture system containing FGF2 and activin A but no BMP4. Our results suggest that the functional outcome of FGF/ERK1/2 signaling in human ES cells is influenced by the relative levels of activin A/TGFbeta and BMP activity. Moreover, activation of ERK1/2 in human ES cells is required for proper neural and mesendodermal differentiation. In contrast to mouse ES cells, a low level of BMP4 is sufficient to initiate extraembryonic differentiation when ERK1/2 is inhibited.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Na J,Furue MK,Andrews PWdoi
10.1016/j.scr.2010.06.002subject
Has Abstractpub_date
2010-09-01 00:00:00pages
157-69issue
2eissn
1873-5061issn
1876-7753pii
S1873-5061(10)00073-5journal_volume
5pub_type
杂志文章abstract：:Human lymphoblast cells from a female and male patient diagnosed with Alzheimer's disease (AD) with different genotypes of a functional copy number variation (CNV) in the AD risk gene CR1 were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal plasmids expressing OCT4, SOX2, NA...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.10.003
更新日期：2016-11-01 00:00:00
abstract：:Two iPSC clones, NCCSi008-A and NCCSi008-B, were generated from a healthy male individual of Indian origin by reprogramming his CD4+ T cells with an integration free Sendai viral vector. The established iPSC clones showed high alkaline phosphatase (ALP) activity, expression of pluripotency markers, a normal male karyo...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.101765
更新日期：2020-05-01 00:00:00
abstract：:The CD71/Ter119 combination has been widely used to reflect dynamic maturation of erythrocytes in vivo. However, because CD71 is expressed on all proliferating cells, it is unclear whether it can be utilized as an erythrocyte-specific marker during differentiation of embryonic stem cells (ESCs). In this study, we reve...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.11.002
更新日期：2015-01-01 00:00:00
abstract：:The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated h...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102126
更新日期：2020-12-16 00:00:00
abstract：:Peripheral blood mononuclear cells (PBMCs) were collected from a 6-year-old female child who was clinically diagnosed as primary nephrotic syndrome (NS) with hormone resistance. An iPSC line was successfully established by the Sendai-virus (SeV) delivery system. The iPS-19 (GSPHi001-A) expressed pluripotent markers, e...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101661
更新日期：2019-12-01 00:00:00
abstract：:Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.09.001
更新日期：2012-03-01 00:00:00
abstract：:Rubinstein-Taybi syndrome (RSTS) is a neurodevelopmental disorder characterized by growth retardation, skeletal anomalies and intellectual disability, caused by heterozygous mutations in either CREBBP (RSTS1) or EP300 (RSTS2) genes. We characterized 3 iPSC lines generated by Sendai from blood of RSTS1 patients with un...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101553
更新日期：2019-10-01 00:00:00
abstract：:We studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors we...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2010.09.002
更新日期：2011-01-01 00:00:00
abstract：:In the present study, we investigated the effect of simultaneous downregulation of uPAR and cathepsin B (pUC), alone or in combination with radiation, on JNK-MAPK signaling pathway in regulating the migration of non-GICs (glioma-initiating cells) and GICs. The increase in the expression of p-JNK with pUC treatment was...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.02.008
更新日期：2014-05-01 00:00:00
abstract：:Bone marrow stromal cell (BMSC) adhesion and migration are fundamental to a number of pathophysiologic processes, including fracture and wound healing. Vitamin C is beneficial for bone formation, fracture repair and wound healing. However, the role of the vitamin C transporter in BMSC adhesion, migration and wound hea...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2013.11.002
更新日期：2014-03-01 00:00:00
abstract：:Parental origin-dependent expression of the imprinted genes is essential for mammalian development. Zfp57 maintains genomic imprinting in mouse embryos and ES cells. To examine the allelic expression patterns of the imprinted genes in ES cells, we obtained multiple hybrid ES clones that were directly derived from the ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.01.017
更新日期：2016-03-01 00:00:00
abstract：:Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102069
更新日期：2020-12-01 00:00:00
abstract：:SOX4 has been shown to promote neuronal differentiation both in the adult and embryonic neural progenitors. Ectopic SOX4 expression has also been shown to inhibit oligodendrocyte differentiation in mice, however the underlying molecular mechanisms remain poorly understood. Here we demonstrate that SOX4 regulates trans...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.10.005
更新日期：2018-12-01 00:00:00
abstract：:The human iPSC cell line, CARS-FiPS4F1 (ESi064-A), derived from dermal fibroblast from the apparently healthy carrier of the mutation of the gene SACSIN, was generated by non-integrative reprogramming technology using OCT3/4, SOX2, CMYC and KLF4 reprogramming factors. The pluripotency was assessed by immunocytochemist...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.10.016
更新日期：2018-12-01 00:00:00
abstract：:The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propag...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.01.007
更新日期：2016-03-01 00:00:00
abstract：:Asparagine synthetase (ASNS) deficiency (ASNSD; MIM #615574) is a rare neurodevelopmental disorder caused by mutations in the ASNS gene. The ASNS gene maps to cytogenetic band 7q21.3 and is 35 kb long. ASNSD is characterised by congenital microcephaly, severely delayed psychomotor development, seizures, and hyperekple...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101583
更新日期：2019-12-01 00:00:00
abstract：:A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integra...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.05.007
更新日期：2018-08-01 00:00:00
abstract：:Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, s...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101590
更新日期：2019-12-01 00:00:00
abstract：:ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endoge...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101631
更新日期：2019-12-01 00:00:00
abstract：:The niche concept of stem cell biology proposes a functional unit between the precursor cells and their local microenvironment, to which several cell types might contribute by cell-cell contacts, extracellular matrix, and humoral factors. We here established three co-culture models (with cell types separated by membra...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.09.010
更新日期：2015-11-01 00:00:00
abstract：:Satellite cells (SCs) are the resident stem cells of skeletal muscle tissue which play a major role in muscle adaptation, e.g. as a response to physical training. The aim of this study was to examine the effects of an intermittent lactate (La) treatment on the proliferation and differentiation of C2C12 myoblasts, simu...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.03.004
更新日期：2014-05-01 00:00:00
abstract：:GABAergic interneuron dysfunction has been implicated in temporal lobe epilepsy (TLE), autism, and schizophrenia. Inhibitory interneuron progenitors transplanted into the hippocampus of rodents with TLE provide varying degrees of seizure suppression. We investigated whether human embryonic stem cell (hESC)-derived int...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.10.007
更新日期：2018-12-01 00:00:00
abstract：:Loeys-Dietz syndrome (LDS) is a rare connective tissue disorder characterized by a genetic predisposition for thoracic aortic aneurysm and dissection. Despite heterozygous loss-of-function mutations in genes for ligand, receptor, or downstream mediators of the transforming growth factor β (TGFβ) pathway, LDS is associ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.101707
更新日期：2020-03-01 00:00:00
abstract：:Neural stem cells (NSCs) have been considered as potential therapy in Alzheimer's disease (AD) but their use is hampered by the poor survival of grafted cells. Supply of neurotrophic factors to the grafted cells has been proposed as a way to augment survival of the stem cells. In this context, we investigated the util...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.04.008
更新日期：2015-07-01 00:00:00
abstract：:Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.04.015
更新日期：2016-05-01 00:00:00
abstract：:Insulin-like growth factor 1 (IGF-1) plays an important role in the regulation of tooth root development, and stem cells from apical papilla (SCAPs) are responsible for the formation of root pulp and dentin. To date, it remains unclear whether IGF-1 can regulate the function of SCAPs. In this study, SCAPs were isolate...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.12.005
更新日期：2012-05-01 00:00:00
abstract：:The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Plurip...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.12.005
更新日期：2017-01-01 00:00:00
abstract：:Two distinct types of embryonic pluripotent stem cells can be established from either the inner cell mass (ICM) of preimplantation blastocyst (leukemia inhibitory factor (LIF)-dependent embryonic stem cell, ESC, called naive state) or the epiblast of postimplantation fetuses (fibroblast growth factor 2 (FGF2)-dependen...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.04.012
更新日期：2014-07-01 00:00:00
abstract：:Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was con...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.12.002
更新日期：2017-01-01 00:00:00
abstract：:Maple syrup urine disease type Ib (MSUD Ib) is an autosomal recessive genetic metabolic disease caused by homozygous or compound heterozygous mutation in BCKDHB on chromosome 6q14. We generated an induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMCs) of a 5-day-old boy with MSUD Ib ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101579
更新日期：2019-10-01 00:00:00
