Abstract:
:The niche concept of stem cell biology proposes a functional unit between the precursor cells and their local microenvironment, to which several cell types might contribute by cell-cell contacts, extracellular matrix, and humoral factors. We here established three co-culture models (with cell types separated by membrane) for both adherent monolayers and neurospheres to address the potential influence of different niche cell types in the neurogenic zone of the adult hippocampus of mice. Astrocytes and endothelial cells enhanced precursor cell proliferation and neurosphere formation. Endothelial factors also led to a prolonged increase in proliferation after growth factor withdrawal, which otherwise induces differentiation. All niche cell types enhanced cell survival in monolayer cultures, endothelial cells also stimulated neuronal differentiation. A parallel trend elicited by astrocytes did not reach conventional statistical significance. Pericytes had variable effects here. We did not observe changes in differentiation in neurosphere co-cultures. In summary, our data indicate that in precursor cell culture protocols survival could be improved by adding as yet unknown factors physiologically contributed by astrocytes and endothelial cells. Our findings also underscore the complexity of the niche and the differential impact of factors from the different sources on distinct aspects of neuronal development. With the help of the models presented here, identification of these factors and their specific biological activity can now be initiated.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Ehret F,Vogler S,Kempermann Gdoi
10.1016/j.scr.2015.09.010subject
Has Abstractpub_date
2015-11-01 00:00:00pages
514-521issue
3eissn
1873-5061issn
1876-7753pii
S1873-5061(15)00129-4journal_volume
15pub_type
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.11.006
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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doi:10.1016/j.scr.2013.09.006
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doi:10.1016/j.scr.2019.101633
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章
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