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Lactate regulates myogenesis in C2C12 myoblasts in vitro.

Abstract：

:Satellite cells (SCs) are the resident stem cells of skeletal muscle tissue which play a major role in muscle adaptation, e.g. as a response to physical training. The aim of this study was to examine the effects of an intermittent lactate (La) treatment on the proliferation and differentiation of C2C12 myoblasts, simulating a microcycle of high intensity endurance training. Furthermore, the involvement of reactive oxygen species (ROS) in this context was examined. C2C12 myoblasts were therefore repeatedly incubated for 2 h each day with 10 mM or 20 mM La differentiation medium (DM) and in some cases 20 mM La DM plus different antioxidative substances for up to 5 days. La free (0 mM) DM served as a control. Immunocytochemical staining, Western blot analysis and colorimetric assays were used to assess oxidative stress, proliferation, and differentiation. Results show that La induces oxidative stress, enhances cell-cycle withdrawal, and initiates early differentiation but delays late differentiation in a timely and dose-dependent manner. These effects can be reversed by the addition of antioxidants to the La DM. We therefore conclude that La has a regulatory role in C2C12 myogenesis via a ROS-sensitive mechanism which elicits implications for reassessing some aspects of training and the use of nutritional supplements.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Willkomm L,Schubert S,Jung R,Elsen M,Borde J,Gehlert S,Suhr F,Bloch W

doi

10.1016/j.scr.2014.03.004

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

742-53

issue

3

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(14)00031-2

journal_volume

12

pub_type

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