Abstract：
:Induced-pluripotent stem cells (iPSCs) are a potential alternative cell source in regenerative medicine, which includes the use of differentiated iPSCs for cell therapies to treat coronary artery and/or peripheral arterial diseases. Late-outgrowth endothelial progenitor cells (late-EPCs) are a unique primary cell present in peripheral blood that exhibit high proliferative capacity, are being used in a wide variety of clinical trials, and have the ability to differentiate into mature endothelial cells. The objective of this study was to reprogram peripheral blood-derived late-EPCs to a pluripotent state under feeder-free and defined culture conditions. Late-EPCs that were retrovirally transduced with OCT4, SOX2, KLF4, c-MYC, and iPSC colonies were derived in feeder-free and defined media conditions. EPC-iPSCs expressed pluripotent markers, were capable of differentiating to cells from all three germ-layers, and retained a normal karyotype. Transcriptome analyses demonstrated that EPC-iPSCs exhibit a global gene expression profile similar to human embryonic stem cells (hESCs). We have generated iPSCs from late-EPCs under feeder-free conditions. Thus, peripheral blood-derived late-outgrowth EPCs represent an alternative cell source for generating iPSCs.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Chang WY,Lavoie JR,Kwon SY,Chen Z,Manias JL,Behbahani J,Ling V,Kandel RA,Stewart DJ,Stanford WLdoi
10.1016/j.scr.2012.11.006subject
Has Abstractpub_date
2013-03-01 00:00:00pages
195-202issue
2eissn
1873-5061issn
1876-7753pii
S1873-5061(12)00119-5journal_volume
10pub_type
杂志文章abstract：:Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available ...
journal_title：Stem cell research
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abstract：:Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous kn...
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journal_title：Stem cell research
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doi：10.1016/j.scr.2013.07.005
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abstract：:Skin fibroblasts were obtained from a 47-year-old hereditary spastic paraplegia patient carrying a homozygous mutation Y275X in CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1), responsible for causing hereditary spastic paraplegia type 5 (SPG5). Induced pluripotent stem cells (iPSCs) were generated by t...
journal_title：Stem cell research
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abstract：:Dominant deafness-onychodystrophy (DDOD) syndrome is a rare, autosomal dominant inherited disorder with no concrete therapies in human. We previously identified c.1516 C > T (p.Arg506*) in ATP6V1B2 as cause of DDOD syndrome, accounting for all cases of this genetic disorder. The induced pluripotent stem cell (iPSC) li...
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journal_title：Stem cell research
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journal_title：Stem cell research
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doi：10.1016/j.scr.2017.11.018
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journal_title：Stem cell research
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.07.002
更新日期：2015-09-01 00:00:00
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.04.010
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journal_title：Stem cell research
pub_type： 杂志文章
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journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2008.11.004
更新日期：2009-03-01 00:00:00
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journal_title：Stem cell research
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doi：10.1016/j.scr.2019.101583
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journal_title：Stem cell research
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journal_title：Stem cell research
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journal_title：Stem cell research
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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journal_title：Stem cell research
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journal_title：Stem cell research
pub_type： 杂志文章
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journal_title：Stem cell research
pub_type： 杂志文章
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abstract：:Neural progenitor cells (NPCs) are promising candidates for cell-based therapy of neurodegenerative diseases; however, safety concerns must be addressed through transplantation studies in large animal models, such as the pig. The aim of this study was to derive NPCs from porcine blastocysts and evaluate their in-vitro...
journal_title：Stem cell research
pub_type： 杂志文章
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