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Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system.

Abstract：

:Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) line using the CRISPR/Cas9 genome-editing technology. We believe that this cell line will be a good resource for exploring the function of the MEIS1 gene in embryonic development in vitro. Furthermore, the gene-knockout method reported in this study is efficient and labor-saving, which may provide an effective strategy for hESC gene deletion.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Zhang C,Yu Y,Li F,Lan X,Wang L

doi

10.1016/j.scr.2020.102069

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

102069

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(20)30370-6

journal_volume

49

pub_type

杂志文章

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