Abstract:
:Both BMP and Wnt signaling control stem cells in bulge/dermal papilla, intestinal crypt, and bone marrow. To explore their roles in the limbal niche, which govern corneal epithelial homeostasis, we established an in vitro model of sphere growth by reunion between single limbal epithelial progenitor cells (LEPCs) and aggregates of limbal niche cells (LNCs) in 3D Matrigel. Compared to LEPCs alone, spheres formed by LEPC+LNC exhibited higher clonal growth and less corneal epithelial differentiation. Furthermore, pSmad1/5/8 was in the nucleus of LEPCs, but not LNCs, and correlated with upregulation of BMP1, BMP3, BMP4, all three BMP receptors, and BMP target genes. Inactivation of BMP signaling in LNCs was correlated with upregulation of noggin preferentially expressed by LNCs. Additionally, β-catenin was stabilized in the perinuclear cytoplasm in LEPCs and correlated with upregulation of Wnt7A and FZD5 preferentially expressed by LEPCs. Inactivation of Wnt signaling in LNCs was correlated with upregulation of DKK1/2 by LNCs. Addition of XAV939 that expectedly downregulated perinuclear β-catenin in LEPCs led to significant reduction of epithelial clonal growth, but upregulated all three BMP receptors and downregulated LNC-derived noggin, resulting in activation of BMP signaling in LNCs. Addition of noggin that expectedly downregulated nuclear localization of pSmad1/5/8 in LEPCs led to nuclear localization of β-catenin in larger LEPCs but membrane relocation of β-catenin in smaller LEPCs and significant upregulation of DKK1/2. Hence, balancing acts between Wnt signaling and BMP signaling exist not only within LEPCs but also between LEPCs and LNCs to regulate clonal growth of LEPCs.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Han B,Chen SY,Zhu YT,Tseng SCdoi
10.1016/j.scr.2014.01.003subject
Has Abstractpub_date
2014-03-01 00:00:00pages
562-73issue
2eissn
1873-5061issn
1876-7753pii
S1873-5061(14)00006-3journal_volume
12pub_type
杂志文章abstract::Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.04.015
更新日期:2016-05-01 00:00:00
abstract::Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was con...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.12.002
更新日期:2017-01-01 00:00:00
abstract::Neuroblasts represent the predominant migrating cell type in the adult mouse brain. There are, however, increasing evidences of migration of other neural precursors. This work aims at identifying in vivo endogenous early neural precursors, different from neuroblasts, able to migrate in response to brain injuries. The ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.11.006
更新日期:2015-01-01 00:00:00
abstract::An important practical limitation of the three-dimensional geometry of stem-cell derived intestinal organoids is that it prevents easy access to the apical epithelium for testing food components, microorganisms, bioactive and toxic compounds. To this end, we here report on a new robust method for generating confluent ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.02.013
更新日期:2018-04-01 00:00:00
abstract::The Genea016 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype and female Allele pattern through traditional karyotyping...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.11.009
更新日期:2016-01-01 00:00:00
abstract::Dominant deafness-onychodystrophy (DDOD) syndrome is a rare, autosomal dominant inherited disorder with no concrete therapies in human. We previously identified c.1516 C > T (p.Arg506*) in ATP6V1B2 as cause of DDOD syndrome, accounting for all cases of this genetic disorder. The induced pluripotent stem cell (iPSC) li...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101986
更新日期:2020-10-01 00:00:00
abstract::Gastrointestinal (GI) homeostasis requires the action of multiple pathways. There is some controversy regarding whether small intestine (SI) Paneth cells (PCs) play a central role in orchestrating crypt architecture and their relationship with Lgr5+ve stem cells. Nevertheless, we previously showed that germline CSF-1 ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2012.12.001
更新日期:2013-03-01 00:00:00
abstract::Human lymphoblast cells from a female and male patient diagnosed with Alzheimer's disease (AD) with different genotypes of a functional copy number variation (CNV) in the AD risk gene CR1 were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal plasmids expressing OCT4, SOX2, NA...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.10.003
更新日期:2016-11-01 00:00:00
abstract::Laminins are one of the major protein groups in the extracellular matrix (ECM) and specific laminin isoforms are crucial for neuronal functions in the central nervous system in vivo. In the present study, we compared recombinant human laminin isoforms (LN211, LN332, LN411, LN511, and LN521) and laminin isoform fragmen...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.09.002
更新日期:2017-10-01 00:00:00
abstract::The Mesenchymal-to-Epithelial Transition (MET) has been recognized as a crucial step for successful reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs). Thus, it has been demonstrated, that the efficiency of reprogramming can be enhanced by promoting an epithelial expression program in cells, with a...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.11.003
更新日期:2015-01-01 00:00:00
abstract::Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. He...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101475
更新日期:2019-07-01 00:00:00
abstract::Angelman syndrome (AS) is a neurodevelopmental disorder with leading symptoms of happy demeanor, intellectual disability, ataxia and seizures. AS can be caused by genetic and epigenetic aberrations, resulting in the absence of functional UBE3A protein in the brain. UBE3A is an imprinted gene, which is, in neurons of t...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.09.015
更新日期:2018-12-01 00:00:00
abstract::Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG-repeat expanding mutation in ATXN3. We generated induced pluripotent stem cells (iPSCs) from a SCA3 patient by electroporation of dermal fibroblasts with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 an...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.02.040
更新日期:2016-05-01 00:00:00
abstract::Cardiomyocyte proliferation and progenitor differentiation are endogenous mechanisms of myocardial development. Cardiomyocytes continue to proliferate in mammals for part of post-natal development. In adult mammals under homeostatic conditions, cardiomyocytes proliferate at an extremely low rate. Because the mechanism...
journal_title:Stem cell research
pub_type: 杂志文章,评审
doi:10.1016/j.scr.2014.09.003
更新日期:2014-11-01 00:00:00
abstract::Androgen-deprivation is a mainstay of therapy for advanced prostate cancer but tumor regression is usually incomplete and temporary because of androgen-independent cells in the tumor. It has been speculated that these tumor cells resemble the stem/progenitor cells of the normal prostate. The purpose of this study was ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.04.005
更新日期:2014-07-01 00:00:00
abstract::Large-scale production of human induced pluripotent stem cells (hiPSCs) by robust and economic methods has been one of the major challenges for translational realization of hiPSC technology. Here we demonstrate a scalable culture system for hiPSC expansion using the E8 chemically defined and xeno-free medium under eit...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.07.011
更新日期:2013-11-01 00:00:00
abstract::Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disordersmay yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a ne...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102038
更新日期:2020-12-01 00:00:00
abstract::Parental origin-dependent expression of the imprinted genes is essential for mammalian development. Zfp57 maintains genomic imprinting in mouse embryos and ES cells. To examine the allelic expression patterns of the imprinted genes in ES cells, we obtained multiple hybrid ES clones that were directly derived from the ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.01.017
更新日期:2016-03-01 00:00:00
abstract::Wolfram Syndrome is a rare, autosomal recessive genetic disorder with clinical symptoms appearing in early childhood. Here, we report a generation of iPSCs from fibroblasts of a patient affected by this disease. Induced pluripotent cells obtained with the application of integration-free episomal vectors display a norm...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101858
更新日期:2020-07-01 00:00:00
abstract::Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting the osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent a valid therapeutic substitute. The aim of this study was to investigate the e...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101463
更新日期:2019-07-01 00:00:00
abstract::Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and generated isogenic iPSC l...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.11.010
更新日期:2014-03-01 00:00:00
abstract::Human iPSC line, iPSC-ADM01(SYSUi001-A), was generated from a 70-year-old male patient with sporadic Alzheimer's disease, using non-integrative reprogramming method. This cell line shows pluripotency both in vitro and in vivo, and has a normal karyotype. ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.101375
更新日期:2019-03-01 00:00:00
abstract::Maturity onset diabetes of the young (MODY) is a hereditary form of diabetes mellitus presenting at childhood or adolescence, which eventually leads to pancreatic β-cells dysfunction. The underlying genetic basis of MODY disorders is haploinsufficiency, where loss-of-function mutations in a single allele cause the dia...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.06.013
更新日期:2018-08-01 00:00:00
abstract::Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, s...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101590
更新日期:2019-12-01 00:00:00
abstract::Biliary atresia (BA) is a common cause of pediatric end-stage liver disease. While its etiology is not yet clear, evidence has suggested that BA results from interactions between genetic susceptibility and environmental factors. Disease relevant human cellular models of BA will facilitate identification of both geneti...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.08.001
更新日期:2017-10-01 00:00:00
abstract::The purpose of this study is to establish a murine embryonic stem cell (mESC) line for isolation of functional ventricular cardiomyocytes (VCMs) and then to characterize the derived VCMs. By crossing the myosin light chain 2v (Mlc2v)-Cre mouse line with the reporter strain Rosa26-yellow fluorescent protein (YFP), we g...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2011.08.004
更新日期:2012-01-01 00:00:00
abstract::Transgene free UNIPDi002-A-human induced pluripotent stem cell (hiPSC) line was generated by Sendai Virus Vectors reprogramming from human oral mucosal epithelial stem cells (hOMESCs) of a patient affected by ectrodactyly-ectodermal dysplasia-clefting (EEC)-syndrome, carrying a mutation in exon 8 of the TP63 gene (R30...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.02.006
更新日期:2018-04-01 00:00:00
abstract::Endothelial progenitor cells (EPCs) may contribute to neurovascular repair after stroke and neurodegeneration. A key step in this process should involve adhesive interactions between EPCs and the targeted cerebral endothelium. Here, we tested the hypothesis that reactive astrocytes may play a critical role in enhancin...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.12.008
更新日期:2014-03-01 00:00:00
abstract::An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of com...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.01.007
更新日期:2018-03-01 00:00:00
abstract::Osteogenesis Imperfecta (OI) is a rare autosomal dominant metabolic disorder caused by heterozygous mutations in the COL1A1 or COL1A2 genes, which encode the pro-α1(I) and pro-α2(I) chains of type I procollagen, respectively. A human induced pluripotent stem cell (iPSC) line, termed as CHFUi001-A, was generated from p...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101907
更新日期:2020-07-05 00:00:00