Abstract:
:BRCA1 germline mutation confers hereditary predisposition for breast and ovarian cancer. To understand the physiopathology of mammary and ovarian epithelial cancer transformation, and to identify early driver molecular events, we have generated an iPSC line from a patient carrying a germline exon 17 deletion in BRCA1 gene (BRAC1Ex17 iPSC) in a high-risk family context. Blood cells were reprogrammed used non-integrative virus of Sendaï. The BRCA1-deleted iPSC had normal karyotype, harboured a deletion in the exon 17 of the BRCA1 gene, expressed pluripotent hallmarks and had the differentiation capacity into the three germ layers.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Griscelli F,Oudrhiri N,Feraud O,Divers D,Portier L,Turhan AG,Bennaceur Griscelli Adoi
10.1016/j.scr.2017.09.003subject
Has Abstractpub_date
2017-10-01 00:00:00pages
135-138eissn
1873-5061issn
1876-7753pii
S1873-5061(17)30188-5journal_volume
24pub_type
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