Abstract：
:Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and generated isogenic iPSC lines by reprogramming. The gene expression and methylation profile of three lines were compared to the parental line and intermediate NPC population. We found no gene probe with expression that differed significantly between hESC and iPSC samples under undifferentiated or differentiated conditions. Analysis of the global methylation pattern also showed no significant difference between the two PSC populations. Both undifferentiated populations were distinctly different from the intermediate NPC population in both gene expression and methylation profiles. One point to note is that H1 is a male line and so extrapolation to female lines should be cautioned. However, these data confirm our previous findings that there are no significant differences between hESCs and hiPSCs at the gene expression or methylation level.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Mallon BS,Hamilton RS,Kozhich OA,Johnson KR,Fann YC,Rao MS,Robey PGdoi
10.1016/j.scr.2013.11.010subject
Has Abstractpub_date
2014-03-01 00:00:00pages
376-86issue
2eissn
1873-5061issn
1876-7753pii
S1873-5061(13)00172-4journal_volume
12pub_type
杂志文章abstract：:An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of com...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.01.007
更新日期：2018-03-01 00:00:00
abstract：:We have generated iPSCs from peripheral blood mononuclear cells (PBMCs) of a healthy man using heat sensitive and non-integrative Sendai virus containing Sox2, Oct3/4, c-Myc and Klf4. Human GRX-MCiPS4F-A2 cell line was established and characterized through this study. ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.07.002
更新日期：2015-09-01 00:00:00
abstract：:Oligodendrocytes, which are the main cell type in cerebral white matter, are generated from their precursor cells (oligodendrocyte precursor cells: OPCs). However, the differentiation from OPCs to oligodendrocytes is disturbed under stressed conditions. Therefore, drugs that can improve oligodendrocyte regeneration ma...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.05.001
更新日期：2015-07-01 00:00:00
abstract：:Frontotemporal dementia with parkinsonism linked to chromosome 17q21.2 (FTDP-17) is an autosomal-dominant neurodegenerative disorder. Mutations in the MAPT (microtubule-associated protein tau) gene can cause FTDP-17, but the underlying pathomechanisms of the disease are still unknown. Induced pluripotent stem cells (i...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.09.024
更新日期：2016-11-01 00:00:00
abstract：:Human embryonic stem cells (hESCs) offer new avenues for studying human development and disease progression in addition to their tremendous potential toward development of cell-replacement therapies for various cellular disorders. We have earlier reported the derivation and characterization of Relicell(®) hES1, the fi...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2010.07.001
更新日期：2010-11-01 00:00:00
abstract：:Umbilical cord blood (UCB) transplantation has emerged as a promising therapy, but it is challenged by scarcity of stem cells. Eltrombopag is a non-peptide, thrombopoietin (TPO) receptor agonist, which selectively activates c-Mpl in humans and chimpanzees. We investigated eltrombopag's effects on human UCB hematopoiet...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2012.05.001
更新日期：2012-09-01 00:00:00
abstract：:Skin fibroblasts were obtained from four patients with Williams-Beuren syndrome (WBS) carrying the typical 1.5 Mb or 1.8 Mb deletion at the 7q11.23 genomic region. Induced pluripotent stem cells (iPSCs) were generated by retroviral infection of fibroblasts with polycystronic vectors. The generated iPSC clones ESi059A,...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102087
更新日期：2020-12-01 00:00:00
abstract：:To develop a disease model for the human 'brittle bone' disease, osteogenesis imperfecta, we have used gene editing to produce a facsimile of the patient heterozygous COL1A1 mutation in an established control iPSC line. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiated into...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101449
更新日期：2019-05-01 00:00:00
abstract：:Cell-mediated gene therapy is a possible means to treat muscular dystrophies like Duchenne muscular dystrophy. Autologous patient stem cells can be genetically-corrected and transplanted back into the patient, without causing immunorejection problems. Regenerated muscle fibres derived from these cells will express the...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.05.004
更新日期：2018-07-01 00:00:00
abstract：:Two distinct types of embryonic pluripotent stem cells can be established from either the inner cell mass (ICM) of preimplantation blastocyst (leukemia inhibitory factor (LIF)-dependent embryonic stem cell, ESC, called naive state) or the epiblast of postimplantation fetuses (fibroblast growth factor 2 (FGF2)-dependen...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.04.012
更新日期：2014-07-01 00:00:00
abstract：:The human embryonic stem cell line RCe019-A (RC-15) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a cleavage st...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.04.003
更新日期：2016-05-01 00:00:00
abstract：:Cardiomyocyte proliferation and progenitor differentiation are endogenous mechanisms of myocardial development. Cardiomyocytes continue to proliferate in mammals for part of post-natal development. In adult mammals under homeostatic conditions, cardiomyocytes proliferate at an extremely low rate. Because the mechanism...
journal_title：Stem cell research
pub_type： 杂志文章,评审
doi：10.1016/j.scr.2014.09.003
更新日期：2014-11-01 00:00:00
abstract：:Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiati...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.03.010
更新日期：2017-05-01 00:00:00
abstract：:Conditioned medium (CM) derived from engineered cells often facilitates the cost-effective culture of a variety of stem cells. Growing emphasis on the importance of rigor and reproducibility in lab-based science requires development of best practices approaches, including quality control procedures for the assessment ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101430
更新日期：2019-05-01 00:00:00
abstract：:The Mesenchymal-to-Epithelial Transition (MET) has been recognized as a crucial step for successful reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs). Thus, it has been demonstrated, that the efficiency of reprogramming can be enhanced by promoting an epithelial expression program in cells, with a...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.11.003
更新日期：2015-01-01 00:00:00
abstract：:A recessive mutation in PLA2G6, which is known to cause a heterogeneous neurodegenerative clinical spectrum, has recently been shown to be responsible for autosomal-recessive familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cell...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101432
更新日期：2019-05-01 00:00:00
abstract：:Aside from its role in cell membrane integrity, cholesterol is a key component in steroid hormone production. The vital functions of steroid hormones such as estrogen, testosterone, glucocorticoids (Gcrts) and mineralocorticoids (Mnrts) in perinatal and adult life are well understood; however, their role during early ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.04.010
更新日期：2015-07-01 00:00:00
abstract：:Adult skeletal muscle regeneration relies on the activity of satellite cells residing in the skeletal muscle niche. However, systemic and intrinsic factors decrease the myogenic differentiation potential of satellite cells thereby impairing muscle regeneration. Here we present data showing that late passage C2C12 myob...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.11.018
更新日期：2018-01-01 00:00:00
abstract：:Human embryonic stem (hES) cells have the dual ability to self-renew and differentiate into specialized cell types. However, in order to realize the full potential of these cells it is important to understand how the genes responsible for their unique characteristics are regulated. In this study we examine the regulat...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.10.003
更新日期：2012-03-01 00:00:00
abstract：:Neural stem cells (NSCs) have been considered as potential therapy in Alzheimer's disease (AD) but their use is hampered by the poor survival of grafted cells. Supply of neurotrophic factors to the grafted cells has been proposed as a way to augment survival of the stem cells. In this context, we investigated the util...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2015.04.008
更新日期：2015-07-01 00:00:00
abstract：:Neural progenitor cells (NPCs) are promising candidates for cell-based therapy of neurodegenerative diseases; however, safety concerns must be addressed through transplantation studies in large animal models, such as the pig. The aim of this study was to derive NPCs from porcine blastocysts and evaluate their in-vitro...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2011.04.004
更新日期：2011-09-01 00:00:00
abstract：:SOX4 has been shown to promote neuronal differentiation both in the adult and embryonic neural progenitors. Ectopic SOX4 expression has also been shown to inhibit oligodendrocyte differentiation in mice, however the underlying molecular mechanisms remain poorly understood. Here we demonstrate that SOX4 regulates trans...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.10.005
更新日期：2018-12-01 00:00:00
abstract：:The long QT syndrome type 3 (LQT3) is currently the 3rd most prevalent of the 15 known types of LQT syndrome. Cardiac events in LQT3 are less frequent than LQT1 and LQT2, but more likely to be fatal. LQT3 is caused by mutation in gene SCN5A, which codes for the Nav1.5 Na+ channel. Herein, we have generated a human emb...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2021.102194
更新日期：2021-01-25 00:00:00
abstract：:The cell surface protein Stem Cell Antigen-1 (Sca-1) marks stem or progenitor cells in several murine tissues and is normally upregulated during cancer development. Although the specific function of Sca-1 remains unknown, Sca-1 seems to play a role in proliferation, differentiation and cell migration in a number of ti...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.07.002
更新日期：2017-08-01 00:00:00
abstract：:Large-scale production of human induced pluripotent stem cells (hiPSCs) by robust and economic methods has been one of the major challenges for translational realization of hiPSC technology. Here we demonstrate a scalable culture system for hiPSC expansion using the E8 chemically defined and xeno-free medium under eit...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2013.07.011
更新日期：2013-11-01 00:00:00
abstract：:Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them in a medium supplemented with fetal calf serum and leukemia inhi...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.09.001
更新日期：2017-10-01 00:00:00
abstract：:In the present study, we investigated the effect of simultaneous downregulation of uPAR and cathepsin B (pUC), alone or in combination with radiation, on JNK-MAPK signaling pathway in regulating the migration of non-GICs (glioma-initiating cells) and GICs. The increase in the expression of p-JNK with pUC treatment was...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.02.008
更新日期：2014-05-01 00:00:00
abstract：:An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency marker...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.11.008
更新日期：2018-12-01 00:00:00
abstract：:Spinocerebellar ataxia type-12 (SCA12) is a neurological disorder caused due to triplet (CAG) repeat expansion in 5' UTR of PPP2R2B. It is one of the most prominent SCA-subtype in Indian population and till date no patient specific models have been described. Human-induced-pluripotent-stem cell (HiPSC) based disease m...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.08.008
更新日期：2018-08-01 00:00:00
abstract：:Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. He...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101475
更新日期：2019-07-01 00:00:00
