Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene.

abstract：:Aside from its role in cell membrane integrity, cholesterol is a key component in steroid hormone production. The vital functions of steroid hormones such as estrogen, testosterone, glucocorticoids (Gcrts) and mineralocorticoids (Mnrts) in perinatal and adult life are well understood; however, their role during early ...

journal_title：Stem cell research

authors： Cabral-Teixeira J,Martinez-Fernandez A,Cai W,Terzic A,Mercola M,Willems E

更新日期：2015-07-01 00:00:00

abstract：:Human pluripotent cells such as human embryonic stem cells (hESC) are a great potential source of cells for cell-based therapies; however, directing their differentiation into the desired cell types with high purity remains a challenge. The stem cell microenvironment plays a vital role in directing hESC fate and we ha...

journal_title：Stem cell research

authors： Lee LH,Peerani R,Ungrin M,Joshi C,Kumacheva E,Zandstra P

更新日期：2009-03-01 00:00:00

abstract：:Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying two mutations in plakophilin-2 (PKP2) gene using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. Pluripotency was confirmed i...

journal_title：Stem cell research

authors： Khudiakov A,Kostina D,Zlotina A,Yany N,Sergushichev A,Pervunina T,Tomilin A,Kostareva A,Malashicheva A

更新日期：2017-10-01 00:00:00

abstract：:CHCHD2 mutation has been reported as a potential cause of a rare form of familial Parkinson's disease. Recently, a novel CHCHD2 mutation was identified in a family with Parkinson's disease. The dermal fibroblasts of the patient were obtained and successfully transformed into induced pluripotent stem cells(iPSCs), empl...

journal_title：Stem cell research

authors： Wang Y,Wang Z,Sun H,Mao C,Yang J,Liu Y,Liu H,Zhang S,Zhang J,Xu Y,Shi C

更新日期：2018-10-01 00:00:00

abstract：:FOS is component of the AP-1 complex and has been reported to be involved in many cellular functions, including cell proliferation, differentiation, survival, angiogenesis, hematopoiesis and cancer progress. To further understand the exact role of FOS in these processes, here we created two FOS knockout human embryoni...

journal_title：Stem cell research

authors： Li C,Wang Q,Peng Z,Lin Y,Liu H,Yang X,Li S,Liu X,Chen J

更新日期：2019-08-01 00:00:00

abstract：:Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial disease caused by homoplasmic point mutations in complex I subunit genes of mitochondrial DNA. In this report, we generated an induced pluripotent stem cell (iPSCs) line, TVGH-iPSC-010-09, from the peripheral blood mononuclear cells of ...

journal_title：Stem cell research

authors： Lu HE,Yang YP,Chen YT,Wu YR,Wang CL,Tsai FT,Hwang DK,Lin TC,Chen SJ,Wang AG,Hsieh PCH,Chiou SH

更新日期：2018-04-01 00:00:00

abstract：:Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiati...

journal_title：Stem cell research

authors： Táncos Z,Nemes C,Varga E,Bock I,Rungarunlert S,Tharasanit T,Techakumphu M,Kobolák J,Dinnyés A

更新日期：2017-05-01 00:00:00

abstract：:The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propag...

journal_title：Stem cell research

authors： Hewitson H,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

更新日期：2016-03-01 00:00:00

abstract：:Gene therapy mediated by bone marrow-derived hematopoietic stem cells (BM-HSC) has been widely used in treating genetic deficiencies in both pre-clinical and clinical settings. Using mitotically inactive cell-targeting lentivirus with separate promoters for our gene of interest (the murine MHC class II (MHCII) chapero...

journal_title：Stem cell research

authors： Wang N,Rajasekaran N,Hou T,Mellins ED

更新日期：2014-01-01 00:00:00

abstract：:Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear...

journal_title：Stem cell research

authors： Cheng YC,Huang CY,Ho MC,Hsu YH,Syu SH,Lu HE,Lin HI,Lin CH,Hsieh PCH

更新日期：2018-04-01 00:00:00

abstract：:We generated an induced human pluripotent stem cell line from a child with microcephaly carrying compound heterozygous mutations of TYW1 inherited from healthy parents. This iPS cell line showed typical embryonic stem cell-like morphology, expressed pluripotent markers that comparable to human embryonic stem cells. Mo...

journal_title：Stem cell research

authors： Sun C,Zhou P,Yuan S,Guo R,Zhang Z,Xu Q,Han D,He L,Tang H,Xu K,Hu H,Li N

更新日期：2020-05-01 00:00:00

abstract：:GABAergic interneuron dysfunction has been implicated in temporal lobe epilepsy (TLE), autism, and schizophrenia. Inhibitory interneuron progenitors transplanted into the hippocampus of rodents with TLE provide varying degrees of seizure suppression. We investigated whether human embryonic stem cell (hESC)-derived int...

journal_title：Stem cell research

authors： Anderson NC,Van Zandt MA,Shrestha S,Lawrence DB,Gupta J,Chen CY,Harrsch FA,Boyi T,Dundes CE,Aaron G,Naegele JR,Grabel L

更新日期：2018-12-01 00:00:00

abstract：:To develop cell therapies for damaged nervous tissue with human neural stem/progenitor cells (hNPCs), the risk of an immune response and graft rejection must be considered. There are conflicting results and lack of knowledge concerning the immunocompetence of hNPCs of different origin. Here, we studied the immunogenic...

journal_title：Stem cell research

authors： Liu J,Götherström C,Forsberg M,Samuelsson EB,Wu J,Calzarossa C,Hovatta O,Sundström E,Åkesson E

更新日期：2013-05-01 00:00:00

abstract：:Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...

journal_title：Stem cell research

authors： Zhang C,Yu Y,Li F,Lan X,Wang L

更新日期：2020-12-01 00:00:00

abstract：:The human embryonic stem cell line NYSCFe001-A was derived from a day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe001-A line, registered as NYSCF100 on the NIH reg...

journal_title：Stem cell research

authors： Sevilla A,Forero E,Zimmer M,Martinez H,Reggio K,Paull D,Egli D,Noggle S

更新日期：2018-05-01 00:00:00

abstract：:Androgen-deprivation is a mainstay of therapy for advanced prostate cancer but tumor regression is usually incomplete and temporary because of androgen-independent cells in the tumor. It has been speculated that these tumor cells resemble the stem/progenitor cells of the normal prostate. The purpose of this study was ...

journal_title：Stem cell research

authors： Shi X,Gipp J,Dries M,Bushman W

更新日期：2014-07-01 00:00:00

abstract：:In order to evaluate the functional significance of palmitoylation during multi-potent neural stem/progenitor cell proliferation and differentiation, retinoic acid-induced P19 cells were used in this study as a model system. Cell behaviour was monitored in the presence of the protein palmitoylation inhibitor 2-bromopa...

journal_title：Stem cell research

authors： Chen X,Du Z,Shi W,Wang C,Yang Y,Wang F,Yao Y,He K,Hao A

更新日期：2014-03-01 00:00:00

abstract：:We have established the patient-specific induced pluripotent stem (iPS) cell line CSUASOi004-A by using peripheral blood mononuclear cells (PBMCs) of a retinitis pigmentosa (RP) patient with a PRPF6 gene mutation (c.G2699A:p.R900H). CSUASOi004-A was established by a non-integrative method with four episomal plasmids c...

journal_title：Stem cell research

authors： Zhou Y,Jing Y,Mao S,Liu J,Cui Z,Wang Y,Chen J,Chan HF,Tang S,Chen J

更新日期：2020-05-01 00:00:00

abstract：:We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and...

journal_title：Stem cell research

authors： Vlahos K,Sourris K,Mayberry R,McDonald P,Bruveris FF,Schiesser JV,Bozaoglu K,Lockhart PJ,Stanley EG,Elefanty AG

更新日期：2019-01-01 00:00:00

abstract：:Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was con...

journal_title：Stem cell research

authors： Guo D,Wu F,Liu H,Gao G,Kou S,Yang F,Abbas N,Zhou T,Cai X,Zhang H,Qin D,Li J,Xu K,Li YX

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND:Angiogenesis is an essential step in tissue engineering. MSC exosomes play an important role in angiogenesis. Functional biomolecules in exosomes vested by the culture microenvironment can be transferred to recipient cells and affects their effect. 3D culture can improve the proliferation and activity of MSC...

journal_title：Stem cell research

authors： Gao W,Liang T,He R,Ren J,Yao H,Wang K,Zhu L,Xu Y

更新日期：2020-12-10 00:00:00

abstract：:The Genea079 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying compound heterozygous mutations in the NEB gene, exon 55 deletion & c.15110dupA, indicative of Nemaline Myopathy Type 2 (NEM2). Following ICM outgrowth on inactivated human feeders, karyotype w...

journal_title：Stem cell research

authors： Dumevska B,McKernan R,Goel D,Schmidt U

更新日期：2016-03-01 00:00:00

abstract：:Optimization of pluripotent stem cell expansion and differentiation is facilitated by biological tools that permit non-invasive and dynamic monitoring of pluripotency, and the ability to select for an undifferentiated input cell population. Here we report on the generation and characterisation of clonal human embryoni...

journal_title：Stem cell research

authors： Ovchinnikov DA,Titmarsh DM,Fortuna PR,Hidalgo A,Alharbi S,Whitworth DJ,Cooper-White JJ,Wolvetang EJ

更新日期：2014-09-01 00:00:00

abstract：:Biliary atresia (BA) is a common cause of pediatric end-stage liver disease. While its etiology is not yet clear, evidence has suggested that BA results from interactions between genetic susceptibility and environmental factors. Disease relevant human cellular models of BA will facilitate identification of both geneti...

journal_title：Stem cell research

authors： Tian L,Eldridge L,Chaudhari P,Zhang L,Anders RA,Schwarz KB,Ye Z,Jang YY

更新日期：2017-10-01 00:00:00

abstract：:Hypertrophic cardiomyopathy is an inherited cardiomyopathy with a prevalence of up to 1 in 200, which can result in significant morbidity and mortality. An iPSC line was generated from peripheral blood mononuclear cells obtained from the whole blood of a 58-year-old male with hypertrophic cardiomyopathy who carries th...

journal_title：Stem cell research

authors： Holliday M,Ross SB,Lim S,Semsarian C

更新日期：2018-12-01 00:00:00

abstract：:Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them in a medium supplemented with fetal calf serum and leukemia inhi...

journal_title：Stem cell research

authors： Tapponnier Y,Afanassieff M,Aksoy I,Aubry M,Moulin A,Medjani L,Bouchereau W,Mayère C,Osteil P,Nurse-Francis J,Oikonomakos I,Joly T,Jouneau L,Archilla C,Schmaltz-Panneau B,Peynot N,Barasc H,Pinton A,Lecardonnel J,Gocz

更新日期：2017-10-01 00:00:00

abstract：:In the present study, we investigated the effect of simultaneous downregulation of uPAR and cathepsin B (pUC), alone or in combination with radiation, on JNK-MAPK signaling pathway in regulating the migration of non-GICs (glioma-initiating cells) and GICs. The increase in the expression of p-JNK with pUC treatment was...

journal_title：Stem cell research

authors： Alapati K,Kesanakurti D,Rao JS,Dasari VR

更新日期：2014-05-01 00:00:00

abstract：:We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 10years old patient with primary congenital glaucoma (PCG). The cells were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system and shown to have full differentiation potential. ...

journal_title：Stem cell research

authors： Zhang J,Wu S,Hu M,Liu Q

更新日期：2018-05-01 00:00:00

abstract：:We studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors we...

journal_title：Stem cell research

authors： Scassa ME,Jaquenod de Giusti C,Questa M,Pretre G,Richardson GA,Bluguermann C,Romorini L,Ferrer MF,Sevlever GE,Miriuka SG,Gómez RM

更新日期：2011-01-01 00:00:00

abstract：:An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of com...

journal_title：Stem cell research

authors： Marote A,Pomeshchik Y,Goldwurm S,Collin A,Lamas NJ,Pinto L,Salgado AJ,Roybon L

更新日期：2018-03-01 00:00:00