Abstract：
:The European Bank for induced Pluripotent Stem Cells (EBiSC), a non-profit repository for storage, banking, Quality Control (QC) and subsequent distribution of research-grade human induced Pluripotent Stem Cell (iPSC) lines, has centralised iPSC lines generated internationally across >35 disease areas and made them available to users via the EBiSC Catalogue, for research use (cells.ebisc.org/). Comprehensive datasets are accessible prior to purchase detailing the disease background of the original tissue sample, background of iPSC reprogramming and cell line characterisation data. EBiSC also performs robust QC screening to ensure supply of reliable, well-characterised iPSC lines, compliant with ISO9001:2015 principles. Whole Genome Sequencing data for specific iPSC lines can be downloaded from the European Genome Archive, subject to application to the EBiSC Data Access Committee. The EBiSC Access and Use Agreement, required to be completed prior to shipping, can be downloaded from the website along with specific Cell Line Information Packs; together these documents clarify how EBiSC lines can be used for research and detail any specific Third Party Obligations and/or restrictions for use which may apply. A protocol for how to culture and monitor iPSC lines including implementation of routine cell line screening is also available. A second project phase will continue collecting iPSC lines generated internationally, provide iPSC derived differentiated products using improved automation strategies for upscaling and develop the current services provided by EBiSC, including iPSC reprogramming, gene-editing and characterisation.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Steeg R,Neubauer JC,Müller SC,Ebneth A,Zimmermann Hdoi
10.1016/j.scr.2020.102034subject
Has Abstractpub_date
2020-12-01 00:00:00pages
102034eissn
1873-5061issn
1876-7753pii
S1873-5061(20)30335-4journal_volume
49pub_type
杂志文章abstract：:Hematopoietic stem and progenitor cell (HSPC) transplantation is the paradigm for stem cell therapies. The protocol described here enables quantitative assessment of the body-wide HSPC reconstitution of different mature hematopoietic cells in mice based on their presence in circulating blood. The method determines don...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102145
更新日期：2020-12-29 00:00:00
abstract：:Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid bod...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.02.007
更新日期：2017-04-01 00:00:00
abstract：:Peripheral blood mononuclear cells for reprogramming in this work were donated by a girl with clinically and genetically diagnosed multiminicore disease harboring compound heterozygote mutations of RYR1 gene. Induced pluripotent stem cells (iPSCs) were obtained by non-integrating episomal vectors containing OCT4, SOX2...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.101775
更新日期：2020-05-01 00:00:00
abstract：:The cell surface protein Stem Cell Antigen-1 (Sca-1) marks stem or progenitor cells in several murine tissues and is normally upregulated during cancer development. Although the specific function of Sca-1 remains unknown, Sca-1 seems to play a role in proliferation, differentiation and cell migration in a number of ti...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.07.002
更新日期：2017-08-01 00:00:00
abstract：:We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 10years old patient with primary congenital glaucoma (PCG). The cells were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system and shown to have full differentiation potential. ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.04.004
更新日期：2018-05-01 00:00:00
abstract：:We established three iPSC lines from postmortem-cultured fibroblasts derived following the sudden unexpected death of an 8-year-old girl with Lennox-Gastaut syndrome, who turned out to have the R551H-mutant STXBP1 gene. These iPSC clones showed pluripotent characteristics while retaining the genotype and demonstrated ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101485
更新日期：2019-08-01 00:00:00
abstract：:Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN t...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101659
更新日期：2020-01-01 00:00:00
abstract：:Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous kn...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102121
更新日期：2020-12-10 00:00:00
abstract：:Both BMP and Wnt signaling control stem cells in bulge/dermal papilla, intestinal crypt, and bone marrow. To explore their roles in the limbal niche, which govern corneal epithelial homeostasis, we established an in vitro model of sphere growth by reunion between single limbal epithelial progenitor cells (LEPCs) and a...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.01.003
更新日期：2014-03-01 00:00:00
abstract：:The CD71/Ter119 combination has been widely used to reflect dynamic maturation of erythrocytes in vivo. However, because CD71 is expressed on all proliferating cells, it is unclear whether it can be utilized as an erythrocyte-specific marker during differentiation of embryonic stem cells (ESCs). In this study, we reve...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2014.11.002
更新日期：2015-01-01 00:00:00
abstract：:Cell-mediated gene therapy is a possible means to treat muscular dystrophies like Duchenne muscular dystrophy. Autologous patient stem cells can be genetically-corrected and transplanted back into the patient, without causing immunorejection problems. Regenerated muscle fibres derived from these cells will express the...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.05.004
更新日期：2018-07-01 00:00:00
abstract：:Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of m...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.04.007
更新日期：2016-05-01 00:00:00
abstract：:Human teratoma is a germ cell tumor that contains normal tissues (e.g., hair, skin or cartilage) differentiated from embryonal germ layers. Because of the feature of this tumor, we hypothesized that human teratomas contain multipotent stem cells that can develop into various non-cancerous normal tissues. In this study...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101633
更新日期：2019-12-01 00:00:00
abstract：:Stem cells can differentiate into various body tissues and organs and thus are considered as promising tools for cell therapy and tissue engineering. Early passage stem cells have high differentiation ability compared to late passage stem cells. Thus, it is important to use early passage stem cells in cell therapy. He...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2019.101475
更新日期：2019-07-01 00:00:00
abstract：:Understanding the complex mechanisms that govern the fate decisions of human embryonic stem cells (hESCs) is fundamental to their use in cell replacement therapies. The progress of dissecting these mechanisms will be facilitated by the availability of robust high-throughput screening assays on hESCs. In this study, we...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2010.04.006
更新日期：2010-09-01 00:00:00
abstract：:Human iPSC line, iPSC-ADM01(SYSUi001-A), was generated from a 70-year-old male patient with sporadic Alzheimer's disease, using non-integrative reprogramming method. This cell line shows pluripotency both in vitro and in vivo, and has a normal karyotype. ...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.101375
更新日期：2019-03-01 00:00:00
abstract：:Maturity onset diabetes of the young (MODY) is a hereditary form of diabetes mellitus presenting at childhood or adolescence, which eventually leads to pancreatic β-cells dysfunction. The underlying genetic basis of MODY disorders is haploinsufficiency, where loss-of-function mutations in a single allele cause the dia...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.06.013
更新日期：2018-08-01 00:00:00
abstract：:The Genea079 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying compound heterozygous mutations in the NEB gene, exon 55 deletion & c.15110dupA, indicative of Nemaline Myopathy Type 2 (NEM2). Following ICM outgrowth on inactivated human feeders, karyotype w...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.02.015
更新日期：2016-03-01 00:00:00
abstract：:We studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors we...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2010.09.002
更新日期：2011-01-01 00:00:00
abstract：:An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency marker...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.11.008
更新日期：2018-12-01 00:00:00
abstract：:The Global Alliance for iPSC Therapies (GAiT) is a new initiative to support the implementation and clinical application of therapies derived from pluripotent stem cells to the benefit of patients globally. GAiT's mission is to serve as a central, international resource for those organisations developing therapies fro...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2020.102036
更新日期：2020-12-01 00:00:00
abstract：:Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying two mutations in plakophilin-2 (PKP2) gene using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. Pluripotency was confirmed i...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.08.014
更新日期：2017-10-01 00:00:00
abstract：:SOX4 has been shown to promote neuronal differentiation both in the adult and embryonic neural progenitors. Ectopic SOX4 expression has also been shown to inhibit oligodendrocyte differentiation in mice, however the underlying molecular mechanisms remain poorly understood. Here we demonstrate that SOX4 regulates trans...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.10.005
更新日期：2018-12-01 00:00:00
abstract：:The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propag...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.01.007
更新日期：2016-03-01 00:00:00
abstract：:An induced pluripotent stem cell (iPSC) line was generated from a 36-year-old patient with sporadic Parkinson's disease (PD). Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver OCT3/4, SOX2, c-MYC and KLF4 factors. The generated cell line (CSC-43) exhibits expression of com...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2018.01.007
更新日期：2018-03-01 00:00:00
abstract：:Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprog...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2017.03.022
更新日期：2017-05-01 00:00:00
abstract：:Skin fibroblasts were obtained from a 47-year-old hereditary spastic paraplegia patient carrying a homozygous mutation Y275X in CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1), responsible for causing hereditary spastic paraplegia type 5 (SPG5). Induced pluripotent stem cells (iPSCs) were generated by t...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.09.011
更新日期：2016-09-01 00:00:00
abstract：:The long QT syndrome type 3 (LQT3) is currently the 3rd most prevalent of the 15 known types of LQT syndrome. Cardiac events in LQT3 are less frequent than LQT1 and LQT2, but more likely to be fatal. LQT3 is caused by mutation in gene SCN5A, which codes for the Nav1.5 Na+ channel. Herein, we have generated a human emb...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2021.102194
更新日期：2021-01-25 00:00:00
abstract：:We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) w...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2016.07.007
更新日期：2016-09-01 00:00:00
abstract：:Umbilical cord blood (UCB) transplantation has emerged as a promising therapy, but it is challenged by scarcity of stem cells. Eltrombopag is a non-peptide, thrombopoietin (TPO) receptor agonist, which selectively activates c-Mpl in humans and chimpanzees. We investigated eltrombopag's effects on human UCB hematopoiet...
journal_title：Stem cell research
pub_type： 杂志文章
doi：10.1016/j.scr.2012.05.001
更新日期：2012-09-01 00:00:00
