Abstract:
:The MB49 bladder cancer cell vaccine was effective against bladder cancer in the mice model in previous studies. However, part of the tumors regrew as the vaccine could not eliminate the cancer stem cells (CSCs). MB49 bladder cancer stem cells (MCSCs) were isolated by a combination of the limited dilution method and the serum free culture medium method. MCSCs possessed higher expression of CD133, CD44, OCT4, NANOG, and ABCG2, the ability of differentiation, higher proliferative abilities, lower susceptibility to chemotherapy, greater migration in vitro, and stronger tumorigenic abilities in vivo. Then streptavidin-mouse granulocyte macrophage-colony stimulating factor (SA-mGM-CSF) MCSCs vaccine was prepared. SA-mGM-CSF MCSCs vaccine extended the survival of the mice and inhibited the growth of tumor in protective, therapeutic, memorial and specific immune response experiments. The level of immunoglobulin G and the ratio of dendritic cells and CD4(+) and CD8(+) T cells were highest in the experimental group when compared to those in other four control groups, as well as for the cytotoxicity assay. We demonstrated that SA-mGM-CSF MCSCs vaccine induces an antitumor immune response to metastatic bladder cancer.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Zhu YT,Zhao Z,Fu XY,Luo Y,Lei CY,Chen W,Li F,Pang SY,Chen SS,Tan WLdoi
10.1016/j.scr.2014.04.006subject
Has Abstractpub_date
2014-07-01 00:00:00pages
111-22issue
1eissn
1873-5061issn
1876-7753pii
S1873-5061(14)00042-7journal_volume
13pub_type
杂志文章abstract::Human iPSC line, iPSC-ADM01(SYSUi001-A), was generated from a 70-year-old male patient with sporadic Alzheimer's disease, using non-integrative reprogramming method. This cell line shows pluripotency both in vitro and in vivo, and has a normal karyotype. ...
journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
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abstract:OBJECTIVE:Fecal incontinence reduces the quality of life of many women but has no long-term cure. Research on mesenchymal stem cell (MSC)-based therapies has shown promising results. The primary aim of this study was to evaluate functional recovery after treatment with MSCs in two animal models of anal sphincter injury...
journal_title:Stem cell research
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doi:10.1016/j.scr.2012.10.002
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101590
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.07.014
更新日期:2017-08-01 00:00:00
abstract::By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human emb...
journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
pub_type: 杂志文章,评审
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101506
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2009.02.005
更新日期:2009-05-01 00:00:00
abstract::Laminins are one of the major protein groups in the extracellular matrix (ECM) and specific laminin isoforms are crucial for neuronal functions in the central nervous system in vivo. In the present study, we compared recombinant human laminin isoforms (LN211, LN332, LN411, LN511, and LN521) and laminin isoform fragmen...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.09.002
更新日期:2017-10-01 00:00:00
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.03.004
更新日期:2014-05-01 00:00:00
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.04.012
更新日期:2014-07-01 00:00:00
abstract::Neurogenesis has been shown to occur in the cerebral cortex in adult rats after ischemic stroke. The origin of the newborn neurons is largely unknown. This study aimed to explore cell division in the poststroke penumbral cortex. Adult male Wistar rats were subjected to photothrombotic ring stroke. After repeated deliv...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2008.07.003
更新日期:2009-01-01 00:00:00
abstract::Salisphere-derived adult epithelial cells have been used to improve saliva production of irradiated mouse salivary glands. Importantly, optimization of the cellular composition of salispheres could improve their regenerative capabilities. The Rho Kinase (ROCK) inhibitor, Y27632, has been used to increase the prolifera...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101608
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101475
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abstract::Two iPSC clones, NCCSi008-A and NCCSi008-B, were generated from a healthy male individual of Indian origin by reprogramming his CD4+ T cells with an integration free Sendai viral vector. The established iPSC clones showed high alkaline phosphatase (ALP) activity, expression of pluripotency markers, a normal male karyo...
journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.08.012
更新日期:2016-09-01 00:00:00
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101443
更新日期:2019-05-01 00:00:00
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journal_title:Stem cell research
pub_type: 杂志文章
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journal_title:Stem cell research
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journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2012.11.006
更新日期:2013-03-01 00:00:00
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journal_title:Stem cell research
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