Novel regulators of stem cell fates identified by a multivariate phenotype screen of small compounds on human embryonic stem cell colonies.

abstract：:Osteogenesis Imperfecta (OI) is a rare autosomal dominant metabolic disorder caused by heterozygous mutations in the COL1A1 or COL1A2 genes, which encode the pro-α1(I) and pro-α2(I) chains of type I procollagen, respectively. A human induced pluripotent stem cell (iPSC) line, termed as CHFUi001-A, was generated from p...

journal_title：Stem cell research

authors： Zheng Z,Lu W,Pei Z,Chen J,Yang T,Luo F

更新日期：2020-07-05 00:00:00

abstract：:Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, i...

journal_title：Stem cell research

authors： Chevalier F,Lavergne M,Negroni E,Ferratge S,Carpentier G,Gilbert-Sirieix M,Siñeriz F,Uzan G,Albanese P

更新日期：2014-05-01 00:00:00

abstract：:Human skin fibroblasts were isolated from a 40-year-old hereditary spastic paraplegia patient carrying an intronic splice site mutation (c.1687+2T>A) in SPAST, leading to hereditary spastic paraplegia type 4 (SPG4). Fibroblasts were reprogrammed using episomal plasmids carrying hOCT4, hSOX2, hKLF4, hL-MYC and hLIN28. ...

journal_title：Stem cell research

authors： Hauser S,Erzler M,Theurer Y,Schuster S,Schüle R,Schöls L

更新日期：2016-11-01 00:00:00

abstract：:BRCA1 germline mutation confers hereditary predisposition for breast and ovarian cancer. To understand the physiopathology of mammary and ovarian epithelial cancer transformation, and to identify early driver molecular events, we have generated an iPSC line from a patient carrying a germline exon 17 deletion in BRCA1 ...

journal_title：Stem cell research

authors： Griscelli F,Oudrhiri N,Feraud O,Divers D,Portier L,Turhan AG,Bennaceur Griscelli A

更新日期：2017-10-01 00:00:00

abstract：:Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of esta...

journal_title：Stem cell research

authors： Itoh M,Kawagoe S,Tamai K,Okano HJ,Nakagawa H

更新日期：2016-07-01 00:00:00

abstract：:Dominant deafness-onychodystrophy (DDOD) syndrome is a rare, autosomal dominant inherited disorder with no concrete therapies in human. We previously identified c.1516 C > T (p.Arg506*) in ATP6V1B2 as cause of DDOD syndrome, accounting for all cases of this genetic disorder. The induced pluripotent stem cell (iPSC) li...

journal_title：Stem cell research

authors： Gao X,Qiu SW,Feng ML,Huang SS,Kang DY,Han MY,Dai P,Yuan YY

更新日期：2020-10-01 00:00:00

abstract：:Pluripotency is a cellular state of multiple options. Here, we highlight the potential for self-organization to contribute to stem cell fate computation. A new way of considering regulatory circuitry is presented that describes the expression of each transcription factor (TF) as a branching process that propagates thr...

journal_title：Stem cell research

authors： Halley JD,Smith-Miles K,Winkler DA,Kalkan T,Huang S,Smith A

更新日期：2012-03-01 00:00:00

abstract：:There are currently no reports of identification of stem cells in human gallbladder. The differences between human gallbladder and intrahepatic bile duct (IHBD) cells have also not been explored. The goals of this study were to evaluate if human fetal gallbladder contains a candidate stem cell population and if fetal ...

journal_title：Stem cell research

authors： Manohar R,Li Y,Fohrer H,Guzik L,Stolz DB,Chandran UR,LaFramboise WA,Lagasse E

更新日期：2015-05-01 00:00:00

abstract：:Human embryonic stem cell (hESC) line chHES-478 was derived from abnormal blastocyst diagnosed with albinism after preimplantation genetic diagnosis (PGD) treatment. DNA sequencing analysis confirmed that chHES-478 cell line carried a compound heterozygous mutation, c.896G>A(p.Arg299His) and c.929_930insC(p.Pro310Glnf...

journal_title：Stem cell research

authors： Sun Y,Zhou X,Chen J,Du J,Lu G,Lin G,Ouyang Q

更新日期：2016-11-01 00:00:00

abstract：:Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunesuppressive effects. We cotransplan...

journal_title：Stem cell research

authors： Wu Y,Cao Y,Li X,Xu L,Wang Z,Liu P,Yan P,Liu Z,Wang J,Jiang S,Wu X,Gao C,Da W,Han Z

更新日期：2014-01-01 00:00:00

abstract：:Liver progenitor (oval) cells have enormous potential in the treatment of patients with liver disease using a cell therapy approach, but their use is limited by their scarcity and the number of donor livers from which they can be derived. Bone marrow may be a suitable source. Previously the derivation of oval cells fr...

journal_title：Stem cell research

authors： Tonkin JN,Knight B,Curtis D,Abraham LJ,Yeoh GC

更新日期：2008-09-01 00:00:00

abstract：:Novel and complementary experimental models are required for investigating the molecular mechanisms underlying the resistance to the available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third of patients and need to be deeply investigated. Here, we hav...

journal_title：Stem cell research

authors： Bono F,Mutti V,Piovani G,Minelli A,Mingardi J,Guglielmi A,Fiorentini C,Barbon A,Missale C,Gennarelli M

更新日期：2020-12-01 00:00:00

abstract：:Neuroblasts represent the predominant migrating cell type in the adult mouse brain. There are, however, increasing evidences of migration of other neural precursors. This work aims at identifying in vivo endogenous early neural precursors, different from neuroblasts, able to migrate in response to brain injuries. The ...

journal_title：Stem cell research

authors： Elvira G,García I,Gallo J,Benito M,Montesinos P,Holgado-Martin E,Ayuso-Sacido A,Penadés S,Desco M,Silva A,Garcia-Sanz JA

更新日期：2015-01-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) offer new avenues for studying human development and disease progression in addition to their tremendous potential toward development of cell-replacement therapies for various cellular disorders. We have earlier reported the derivation and characterization of Relicell(®) hES1, the fi...

journal_title：Stem cell research

authors： Mandal A,Bhowmik S,Patki A,Viswanathan C,Majumdar AS

更新日期：2010-11-01 00:00:00

abstract：:As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated wit...

journal_title：Stem cell research

authors： Huo Z,Tu J,Xu A,Li Y,Wang D,Liu M,Zhou R,Zhu D,Lin Y,Gingold JA,Yen CJ,Xiao H,Zhao R

更新日期：2019-01-01 00:00:00

abstract：:The KCL035 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the HBB gene, which is linked to the β-thalassemia syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propag...

journal_title：Stem cell research

authors： Hewitson H,Wood V,Kadeva N,Cornwell G,Codognotto S,Stephenson E,Ilic D

更新日期：2016-03-01 00:00:00

abstract：:Friedreich's ataxia is caused by large homozygous, intronic expansions of GAA repeats in the frataxin (FXN) gene, resulting in severe downregulation of its expression. Pathogenic repeats are located in intron one, hence patients express unaffected FXN protein, albeit in low quantities. Although FRDA symptoms typically...

journal_title：Stem cell research

authors： Li J,Rozwadowska N,Clark A,Fil D,Napierala JS,Napierala M

更新日期：2019-10-01 00:00:00

abstract：:SLC26A4 is the second most frequent gene implicated in congenital hearing loss after GJB2 mutations. Here, we report the generation of induced pluripotent stem cells (iPSCs), from a patient who was carrying a homozygous c.919-2A>G variant in the SLC26A4 gene. This is the most common variant of SLC26A4 gene in the Chin...

journal_title：Stem cell research

authors： Cheng YF,Chan YH,Hu CJ,Lu YC,Saeki T,Hosoya M,Saegusa C,Fujioka M,Okano H,Weng SM,Hsu CJ,Chang KH,Wu CC

更新日期：2019-10-01 00:00:00

abstract：:Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG repeat expansion in the region of the ATXN3 gene. The main feature of SCA3 is progressive ataxia, which affects balance, gait, and speech. Urine cells (UCs) of a SCA3 patient were successfully translated to induced...

journal_title：Stem cell research

authors： Wang Y,Shi C,Wang Z,Sun H,Yang Z,Zhang F,Liu Y,Liu H,Jiang C,Zhang S,Xu Y,Wen X

更新日期：2018-04-01 00:00:00

abstract：:Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting the osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent a valid therapeutic substitute. The aim of this study was to investigate the e...

journal_title：Stem cell research

authors： Niada S,Giannasi C,Gomarasca M,Stanco D,Casati S,Brini AT

更新日期：2019-07-01 00:00:00

abstract：:Crumbs homologue 1 (CRB1) mutations have been found in retinitis pigmentosa (RP) patients lead to severe retinal dystrophies. The human induced pluripotent stem (iPS) cell line CSUASOi003-A derived from peripheral blood mononuclear cells (PBMCs) of a patient carrying two heterozygous mutations (2249G>A p.G750D and c.2...

journal_title：Stem cell research

authors： Zhou Y,Ding C,Xia S,Jing Y,Mao S,Liu J,Chen J,Chan HF,Tang S,Chen J

更新日期：2020-04-01 00:00:00

abstract：:Spinocerebellar ataxia type-12 (SCA12) is a neurological disorder caused due to triplet (CAG) repeat expansion in 5' UTR of PPP2R2B. It is one of the most prominent SCA-subtype in Indian population and till date no patient specific models have been described. Human-induced-pluripotent-stem cell (HiPSC) based disease m...

journal_title：Stem cell research

authors： Kumar D,Hussain A,Srivastava AK,Mukerji M,Mukherjee O,Faruq M

更新日期：2018-08-01 00:00:00

abstract：:Pathological myopia (PM) is a retinal degenerative disease with an increasing prevalence in Asia. The peripheral blood mononuclear cells (PBMCs) from a patient with PM were successfully reprogrammed to induced pluripotent stem cells (iPSCs) using integration-free method, Sendai viral (SeV) vectors expressing OCT4, SOX...

journal_title：Stem cell research

authors： Bai X,Yang X,Cheng Y,Chen L

更新日期：2019-01-01 00:00:00

abstract：:An important practical limitation of the three-dimensional geometry of stem-cell derived intestinal organoids is that it prevents easy access to the apical epithelium for testing food components, microorganisms, bioactive and toxic compounds. To this end, we here report on a new robust method for generating confluent ...

journal_title：Stem cell research

authors： van der Hee B,Loonen LMP,Taverne N,Taverne-Thiele JJ,Smidt H,Wells JM

更新日期：2018-04-01 00:00:00

abstract：:The human iPSC line LEIi006-A was generated from dermal fibroblasts from a patient with retinitis pigmentosa using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. The iPSC cells carry compound heterozygous mutations (c.1892A > G and c.2548G > A) in the CRB1 gene. LEI...

journal_title：Stem cell research

authors： Zhang X,Zhang D,Chen SC,Lamey T,Thompson JA,McLaren T,De Roach JN,Chen FK,McLenachan S

更新日期：2018-08-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 60-year-old female diagnosed with sporadic amyotrophic lateral sclerosis (sALS). The iPSCs shared the same karyotype with the parent PBMCs, expressed pluripotency stem cell markers, and demonstrated tr...

journal_title：Stem cell research

authors： Feng B,Ma J,Amponsah AE,Guo R,Kong D,He J,Jiang Y,Zhang W,Zhang Z,Song Y,Shen S,O'Brien T,Cui H

更新日期：2020-05-01 00:00:00

abstract：:The conventional method of culturing human embryonic stem cells (hESC) is on two-dimensional (2D) surfaces, which is not amenable for scale up to therapeutic quantities in bioreactors. We have developed a facile and robust method for maintaining undifferentiated hESC in three-dimensional (3D) suspension cultures on ma...

journal_title：Stem cell research

authors： Oh SK,Chen AK,Mok Y,Chen X,Lim UM,Chin A,Choo AB,Reuveny S

更新日期：2009-05-01 00:00:00

abstract：:Glycogen debranching enzyme (GDE) plays a critical role in glycogenolysis. Mutations in the GDE gene are associated with a metabolic disease known as glycogen storage disease type III (GSDIII). We generated a mutant GDE human embryonic stem cell line, WAe001-A-14, using the CRISPR/Cas9 editing system. This cell line c...

journal_title：Stem cell research

authors： Xu G,Guo D,Wu F,Abbas N,Lai K,Yuan F,You K,Liu Y,Zhuang Y,Wu Y,Xu Y,Chen Y,Yang F,Pan T,Li YX

更新日期：2018-03-01 00:00:00

abstract：:The Global Alliance for iPSC Therapies (GAiT) is a new initiative to support the implementation and clinical application of therapies derived from pluripotent stem cells to the benefit of patients globally. GAiT's mission is to serve as a central, international resource for those organisations developing therapies fro...

journal_title：Stem cell research

authors： Sullivan S,Ginty P,McMahon S,May M,Solomon SL,Kurtz A,Stacey GN,Bennaceur Griscelli A,Li RA,Barry J,Song J,Turner ML

更新日期：2020-12-01 00:00:00

abstract：:Stem cell differentiation is a complex biological event. Our understanding of this process is partly hampered by the co-existence of different cell subpopulations within a given population, which are characterized by different gene expression states driven by different underlying transcriptional regulatory networks (T...

journal_title：Stem cell research

authors： Okawa S,del Sol A

更新日期：2015-09-01 00:00:00