Abstract:
:The pathogenesis of abdominal aortic aneurysm remains unclear. The aim of the present study was to establish whether isoleucyl-tRNA synthetase (Iars) regulates the differentiation and apoptosis of vascular smooth muscle cells (VSMCs) during the development of abdominal aortic aneurysm (AAA). In addition, the contribution of various signaling pathways towards this process was ascertained. The study demonstrated that the expression of Iars, p-p38, osteopontin (OPN) and Bcl-2-associated X protein (Bax) clearly increased, while levels of p-PI3K and smooth muscle 22 alpha (SM22α) decreased significantly in AAA tissues. Inhibition of Iars significantly reduced the incidence of angiotensin II (AngII)-induced AAA in mice, coincident with decreased activity of the p38 MAPK pathway and increased PI3K pathway activity. AngII-induced phenotypic switching and apoptosis of VSMCs decreased following the inhibition of Iars in vitro. Upregulation of the IARS gene induced phenotypic switching and apoptosis in VSMCs in addition to increased p38 MAPK pathway activation and reduced PI3K pathway activation. Following pretreatment with an activator of the PI3K pathway, expression of Iars and the phenotypic markers of VSMCs were not affected, while apoptosis of VSMCs decreased. Similarly, inhibition of the p38 MAPK pathway in VSMCs did not affect the expression of Iars or the degree of cell apoptosis, but reduced phenotypic switching was observed. Conclusively, upregulation of Iars regulates the phenotypic switching and apoptosis of VSMCs. Targeting Iars may be a promising strategy to prevent abdominal aortic aneurysm.
journal_name
Life Scijournal_title
Life sciencesauthors
Li B,Wang Z,Chen R,Hong J,Wu Q,Hu J,Hu Z,Zhang Mdoi
10.1016/j.lfs.2019.03.052subject
Has Abstractpub_date
2019-05-01 00:00:00pages
51-57eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30216-4journal_volume
224pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Hepatocyte growth factor (HGF) modulates intestinal epithelial cell proliferation and migration. We previously reported that systemic administration of recombinant human HGF (rh-HGF) ameliorated experimental colitis. However, an increase in serum HGF concentrations may induce undesired systemic effects, limiting t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.06.019
更新日期:2011-08-15 00:00:00
abstract::Cancer is a disease of high complexity. Resistance to therapy is a major challenge in cancer targeted therapies. Overcoming this resistance requires a deep knowledge of the cellular interactions within tumor. Natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) are the main anti-cancer immune cells, while T re...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.116960
更新日期:2019-12-01 00:00:00
abstract::Hemin (ferriprotoporphyrin IX-chloride) can mediate the covalent cross-linking and degradation of yeast glutathione reductase. This reaction requires both NADPH and oxygen suggesting the involvement of a reduced oxygen species in the cross-linking and degradation process. During the course of the reaction the enzymati...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90312-1
更新日期:1985-06-03 00:00:00
abstract::Recent studies in animals have demonstrated that the steroid, 3 alpha-hydroxy-5 alpha-pregnan-20-one (3A5P), is a potent analgesic when given intracerebroventricularly. Several studies in humans report that spinal steroids are effective in the treatment of chronic low-back pain when given in combination with morphine....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90095-7
更新日期:1992-01-01 00:00:00
abstract:AIMS:Obesity is not only associated with metabolic diseases but is also a symptom of menopause in women. To date, there are no effective drugs for the management of obesity, and it is important to find new agents with fewer side effects, for the treatment of obesity. This study aimed to determine the anti-obesity effec...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116672
更新日期:2019-09-01 00:00:00
abstract::Galectin-10 (gal-10, also known as Charcot-Leyden crystal protein) is a member of the galectin family of beta-galactoside binding proteins that is expressed uniquely in eosinophilic and basophilic leukocytes. To gain a better understanding of galectin gene expression, we present an analysis of the transcriptional regu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01104-3
更新日期:2001-06-01 00:00:00
abstract::Growth of P. aeruginosa, slowed by the addition of monofluoromethylornithine, difluoromethylarginine and dicyclohexylammonium sulfate, could be restored by addition of 0.1 mM putrescine plus 0.1 muM spermidine, or 0.1 mM spermidine or 5 mM putrescine by themselves. Lower concentrations of putrescine (0.1 mM - 1 mM) al...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90605-2
更新日期:1984-04-16 00:00:00
abstract::Fissistigma oldhamii (Hemsl.) Merr [F. oldhamii], a traditional Chinese herb medicine, is widely used for treating rheumatoid arthritis (RA) in China. Following bioactivity-guided isolation, a representative immunosuppressive compound with low cytotoxicity, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenam...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.10.004
更新日期:2007-12-14 00:00:00
abstract::The objective of this study was to determine the primary event that occurs in Ca2+-regulatory sarcoplasmic-reticular (SR) proteins during subacute transition from concentric/mechanically-compensated left ventricular (LV) hypertrophy to eccentric/decompensated hypertrophy. Using Dahl salt-sensitive rats with hypertensi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01383-2
更新日期:2001-11-30 00:00:00
abstract::The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M +...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90099-3
更新日期:1987-05-25 00:00:00
abstract::The association between various parameters of acute and chronic smoking status and plasma levels of three proteins, C9, C1-inhibitor (C1-INH) and alpha 1-protease inhibitor (alpha 1-PI) were determined for 49 male cigarette smokers and 49 age-matched nonsmokers (mean age = 32.2 years). The mean number of cigarettes sm...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90391-1
更新日期:1985-03-11 00:00:00
abstract::The in vitro oxidative hemolysis of human red blood cells (RBCs) was used as a model to study the free radical-induced damage of biological membranes and the protective effect of flavonols and their glycosides (FOHs), i.e., myricetin (MY), quercetin (Q), morin (MO), kaempferol (K), rutin (R), quercetin galactopyranosi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.10.009
更新日期:2006-04-18 00:00:00
abstract:AIMS:To investigate the roles of neutrophil alkaline phosphatase (NAP) in the migration, reactive oxygen species (ROS) generation and apoptosis of neutrophils. MAIN METHODS:NAP was overexpressed in neutrophil-like differentiated HL-60 cells via transfecting coding sequence of NAP by lentivirus. NAP overexpression in H...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.06.005
更新日期:2016-07-15 00:00:00
abstract::We investigated the effects of ketanserin, a S 2 (5-hydroxytryptamine 2; 5-HT 2)-serotonergic receptor antagonist, on cerulein-induced pancreatitis in the rat. Large pharmacological doses of cerulein induced acute pancreatitis in the rat. Ketanserin reduced the cerulein-induced increase in serum amylase concentration ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90476-6
更新日期:1992-01-01 00:00:00
abstract::The leukotriene (LT) synthesis inhibitors BAY x1005 and MK-886 were evaluated in human lung parenchyma challenged with an anti-IgE. The anti-IgE-induced LTE4 release was time- and dose-dependent. Treatment of the parenchyma with indomethacin (3 microM) prior to anti-IgE challenge inhibited the 6-keto prostaglandin F1 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00426-2
更新日期:1996-01-01 00:00:00
abstract::More than 140 thousands of women suffer from endometrial carcinoma in the worldwide, and over 40 thousand of the patients die before and after in surgery and chemoradiotherapy because of its metastasis. However, its molecular mechanism is much less known compared to other cancers. In this study, we demonstrated that l...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117295
更新日期:2020-02-15 00:00:00
abstract::Brain noradrenaline takes part in the regulation of several brain functions. The formation of brain noradrenaline depends on brain tyrosine (Tyr) levels, which associates with the ratio in plasma of Tyr to other large, neutral amino acids (LNAA). Tyr metabolism has been studied in users of the new generation combined ...
journal_title:Life sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0024-3205(94)00502-j
更新日期:1995-01-01 00:00:00
abstract::The effects of erythropoietin (EPO) on cytosolic free calcium concentration ([Ca2+]i) in platelets of 20 essential hypertensive patients (HT) and of 25 normotensive subjects (NT) were investigated using the fura2 technique. In resting platelets [Ca2+]i were not significantly higher in HT compared to NT (74.3 +/- 7.8 n...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90010-m
更新日期:1992-01-01 00:00:00
abstract::The induction of aldose reductase (AR) mRNA expression and its activity by glucose were investigated in fetal rat aortic smooth muscle (A10) cells. The increase in the expression of AR mRNA was observed at 6 h, and reached a maximum (2.3 fold) at 12 h after exposure to 80 mM glucose. On the other hand, increase in AR ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90480-d
更新日期:1992-01-01 00:00:00
abstract::We have developed an assay for serotonin (5-HT) stimulation of adenylate cyclase activity in membranes from adult guinea pig hippocampus. The response to 5-HT is concentration-dependent, with an EC50 of 0.01 microM, a shallow slope, and mean maximal stimulation of 90% over basal activity. The response to 5-HT is GTP-d...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90763-4
更新日期:1983-05-16 00:00:00
abstract::The accumulation of inositol monophosphate (IP1) was measured after stimulation of 5-hydroxytryptamine2 (5-HT2) receptors on platelets from alcoholics and healthy controls. In controls, 5-HT induced a dose-dependent response with an EC50 = 2 x 10(-6) M and a maximal response at 10(-5) M. Ritanserin, a selective 5-HT2 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90076-8
更新日期:1988-01-01 00:00:00
abstract:AIMS:The progressive accumulation of beta-amyloid peptide (Abeta), in the form of senile plaques, has been recognized as one of the major causes of Alzheimer's disease (AD) pathology. Increased production of Abeta and the aggregation of Abeta to oligomers have been reported to trigger neurotoxicity, oxidative damage an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.12.001
更新日期:2009-02-27 00:00:00
abstract:AIMS:In myocardial ischemia-reperfusion (MI/R) injury, impaired autophagy function worsens cardiomyocyte death. AMP-activated protein kinase (AMPK) is a heterotrimeric protein that plays an important role in cardioprotection and myocardial autophagic function. AMPKα1 and α2 are localized primarily in the cytoplasm and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.04.002
更新日期:2019-05-15 00:00:00
abstract::Vascular disease is one of the complicating features of diabetes mellitus. Magnesium deficiency has recently been proposed as a novel factor implicated in the pathogenesis of diabetes complications. Several studies have indicated that hypertension in diabetic patients is an independent altered reaction of blood vessel...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.07.027
更新日期:2005-02-11 00:00:00
abstract:AIMS:We tested the hypothesis that respiratory quotient (RQ) determines sympathovagal balance associated with metabolism of stored and dietary energy substrates. MAIN METHODS:Six 18-20 year-old African-American males were studied after two control pretreatments of fasting and post-treatments of metabolizing high-fat a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.05.006
更新日期:2009-07-17 00:00:00
abstract::Several experiments were conducted to evaluate the effects of chronic amphetamine on the influenza A (PR-8/34) virus specific immune injury in CD-1 mice. Treatment with amphetamine resulted in a significant increase of lung virus titers and pulmonary vascular permeability. Amphetamine also increased the lethality of i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90520-d
更新日期:1993-01-01 00:00:00
abstract:AIMS:The long non-coding RNA (lncRNA) was reported to be involved in the progress of various cancers, however, its effect in colorectal cancer (CRC) remains unknown. The goal of the present study is to investigate the function role of lncRNA PCAT-1 in colorectal cancer. MAIN METHODS:The expression of lncRNA PCAT-1 in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.08.024
更新日期:2017-11-01 00:00:00
abstract:AIMS:Metformin is an insulin sensitizing agent with beneficial effects in diabetic patients on glycemic levels and in the cardiovascular system. We examined whether the metabolic changes and the vascular dysfunction in monosodium glutamate-induced obese non-diabetic (MSG) rats might be improved by metformin. MAIN METH...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.11.005
更新日期:2012-01-30 00:00:00
abstract::In our previous studies, exposure of mice to millimeter waves (MW) increased the duration of anesthesia caused by either ketamine or chloral hydrate, and this effect was blocked by naloxone. To further characterize the biological effects of MW, we have chosen a new animal model of experimental itch. Male Swiss albino ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00436-6
更新日期:1998-01-01 00:00:00
abstract::The growth response of aortic vascular smooth muscle cells (VSMCs) to chronic hypertension includes vascular hypertrophy. We have shown previously that angiotensin II positively regulates the expression of the human vascular smooth muscle (SM) alpha-actin gene. To further expand our understanding of vasoactive peptide...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00332-3
更新日期:1996-01-01 00:00:00