Calcium handling and sarcoplasmic-reticular protein functions during heart-failure transition in ventricular myocardium from rats with hypertension.

Abstract:

:The objective of this study was to determine the primary event that occurs in Ca2+-regulatory sarcoplasmic-reticular (SR) proteins during subacute transition from concentric/mechanically-compensated left ventricular (LV) hypertrophy to eccentric/decompensated hypertrophy. Using Dahl salt-sensitive rats with hypertension, changes of myocardial contraction, intracellular Ca2+ transients, SR Ca2+ uptake, protein levels of SR Ca2+ ATPase (SERCA2), phospholamban, and calsequestrin (CSQ), and mRNA levels of SERCA2 and CSQ were serially determined and compared between the established stage of LV hypertrophy (LVH) and the subsequent stage of overt LV dysfunction (CHF). In LVH, isolated LV papillary muscle preparations showed an equal peak-tension level and a mild prolongation of the isometric tension decay compared to those of age-matched controls. The Ca2+ transients as measured by aequorin were unchanged. The Ca2+ uptake of isolated SR vesicles and the protein/mRNA levels of SR proteins were also equivalent to those of the controls. In contrast, in CHF, the failing myocardium showed a further prolongation of the contraction time course and a 39% reduction of the peak-tension development. The Ca2+ transients showed changes consisting of a decrease in the peak level and a prolongation of the time course. In addition, the SR Ca2+ uptake was decreased by 41%. Despite these functional changes, the protein and mRNA levels of the SR components remained equivalent to those of the age-matched controls. Thus, in this hypertensive animal, 1) at the LVH stage, myocardial contractility and intracellular capability to regulate Ca2+ remained normal; 2) at the CHF stage, impaired SR Ca2+ handling and the subsequent reduction of myocardial contraction were in progress; and 3) impairments of SR function occurred at the post-translational protein level rather than at the transcriptional/translational levels. Our findings support the role of SR proteins as the primary determinant of the contractile dysfunction that occurs during the heart-failure transition; however, post-translational modulators of these SR elements may also be critical.

journal_name

Life Sci

journal_title

Life sciences

authors

Yoneda T,Kihara Y,Ohkusa T,Iwanaga Y,Inagaki K,Takeuchi Y,Hayashida W,Ueyama T,Hisamatsu Y,Fujita M,Hatac S,Matsuzaki M,Sasayama S

doi

10.1016/s0024-3205(01)01383-2

subject

Has Abstract

pub_date

2001-11-30 00:00:00

pages

143-57

issue

2

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(01)01383-2

journal_volume

70

pub_type

杂志文章
  • Pathways and roadblocks in muscarinic receptor-mediated growth regulation.

    abstract::In some cell systems muscarinic receptor stimulation can induce proliferation or transformation. This phenomenon is subtype-specific (only m1 and m3 receptors are effective) and cell type dependent. In 1321N1 astrocytoma cells activation of m3 receptors stimulates phospholipase C, but does not induce DNA synthesis. In...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Brown JH,Sah V,Moskowitz S,Ramirez T,Collins L,Post G,Goldstein D

    更新日期:1997-01-01 00:00:00

  • Orally administrated rare earth element cerium induces metallothionein synthesis and increases glutathione in the mouse liver.

    abstract::The influence of oral administration of rare earth element cerium (Ce) was studied in relation to metallothionein (MT) and glutathione (GSH) content in the organs of ICR mice, which were administered heavy metal cadmium (Cd) for comparison. Male ICR mice were divided into 9 groups: 1 control group, 4 cerium groups and...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2005.02.004

    authors: Kawagoe M,Hirasawa F,Cun Wang S,Liu Y,Ueno Y,Sugiyama T

    更新日期:2005-07-08 00:00:00

  • Orthovanadate-induced cell death in RET/PTC1-harboring cancer cells involves the activation of caspases and altered signaling through PI3K/Akt/mTOR.

    abstract:AIMS:Oncogenic RET/PTC1 chromosomal rearrangements are hallmarks of thyroid papillary carcinoma. The resulting protein, mainly through tyrosine 451, is responsible for the activation of pathways controlling cell survival, including the PI3K/Akt/mTOR cascade. Vanadium compounds were shown to have anti-neoplastic potenti...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2011.07.004

    authors: Gonçalves AP,Videira A,Soares P,Máximo V

    更新日期:2011-09-12 00:00:00

  • Proenkephalin-A-derived peptides are present in human brain.

    abstract::The opioid peptides [Met] enkephalin-Arg6-Phe7, [Met]-enkephalin-Arg6-Gly7-Leu8, and BAM-12P were identified in various ares of human brain by use of a combination of radioimmunoassay (RIA), gel filtration, and high performance liquid chromatography (HPLC). The highest levels of each peptide were found in striatum, fo...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(83)90439-3

    authors: Pittius CW,Seizinger BR,Mehraein P,Pasi A,Herz A

    更新日期:1983-01-01 00:00:00

  • Labelling of uric acid and allantoin in different purine organs and urine of the rat.

    abstract::We studied the incorporation of 14C-formate into uric acid and allantoin in different organs (liver, lung, kidney, spleen), isolated hepatocytes, perfused liver and urine of the rat. Allantoin had a higher specific radioactivity than uric acid after 14C-formate load in the liver in vivo. This was found to be a strictl...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(96)00387-6

    authors: Pizzichini M,Pandolfi ML,Arezzini L,Terzuoli L,Fe L,Bontemps F,Van den Berghe G,Marinello E

    更新日期:1996-01-01 00:00:00

  • Environmental enrichment reverses cognitive impairments provoked by Western diet in rats: Role of corticosteroid receptors.

    abstract:AIMS:Previous studies demonstrated that the Western diet (WD), which is rich in saturated fat (HFD) and refined sugar (HSU), is related to the impairments of hippocampus-dependent learning and memory and forebrain synaptic plasticity in rodents. The environmental enrichment (EE) has been shown to enhance learning and m...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2016.02.011

    authors: Gergerlioglu HS,Oz M,Demir EA,Nurullahoglu-Atalik KE,Yerlikaya FH

    更新日期:2016-03-01 00:00:00

  • Forkhead box transcription factors in embryonic heart development and congenital heart disease.

    abstract::Embryonic heart development is a very complicated process regulated precisely by a network composed of many genes and signaling pathways in time and space. Forkhead box (Fox, FOX) proteins are a family of transcription factors characterized by the presence of an evolutionary conserved "forkhead"or "winged-helix" DNA-b...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2015.12.001

    authors: Zhu H

    更新日期:2016-01-01 00:00:00

  • An inhibitor of T lymphocyte blastogenesis derived from the unfertilized ova of Shad (Alosa sapidissima).

    abstract::Unfertilized ova from Shad, a North Atlantic herring, contains a cytostatic inhibitor of T lymphocyte blastogenesis. The inhibitor has an estimated molecular weight of 10,000-30,000 Da, is heat stable, non dialyzable, and resistant to protease digestion and periodate oxidation. Although the inhibitor functions at an e...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(90)90336-p

    authors: Sheid B,Nobiletti JB,Prat JC

    更新日期:1990-01-01 00:00:00

  • Pharmacological properties of β-adrenoceptors mediating rat superior mesenteric artery relaxation and the effects of chemical sympathetic denervation.

    abstract:AIMS:β-Adrenoceptors (β-ADRs) mediating the relaxation of rat superior mesenteric arteries (SMAs) were pharmacologically identified, and the effects of chemical sympathetic denervation on β-ADR-mediated relaxation were examined. MAIN METHODS:The tension changes of endothelium-denuded SMAs were isometrically recorded a...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.117155

    authors: Obara K,Shigematsu M,Takahasi H,Iiboshi Y,Yoshioka K,Kasuya Y,Tanaka Y

    更新日期:2020-01-15 00:00:00

  • No evidence for enkephalinase A on neuronal cells.

    abstract::It is generally assumed that enkephalinase A, a highly thiorphan-sensitive dipeptidylcarboxypeptidase cleaving the Gly-Phe bond during enkephalin degradation, is bound to the neuronal membrane. To clarify the localization of the enzyme, we used three neuron-like models (neuroblastoma cells N1E-115, bulk prepared neuro...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(83)90455-1

    authors: Lentzen H,Monden I,Linke J,Palenker J

    更新日期:1983-01-01 00:00:00

  • The effect of ultrasound for increasing neural differentiation in hBM-MSCs and inducing neurogenesis in ischemic stroke model.

    abstract:AIMS:This study's purpose was to evaluate the effect of ultrasound in air at a frequency of 0.04MHz and an intensity of 50mW/cm2 on neural differentiation of hBM-MSCs in vitro and on neurogenesis in an ischemic stroke model. MATERIALS AND METHODS:hBM-MSCs were exposed to 0.04MHz ultrasound and then compared with no ex...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2016.08.029

    authors: Cho SE,Kim YM,Jeong JS,Seo YK

    更新日期:2016-11-15 00:00:00

  • Rapid and simple gas chromatographic measurement of lactic acid in red blood cells, plasma, and tumor cells after hyperthermia.

    abstract::The content of lactic acid in red blood cells, plasma, and Ehrlich ascites tumor cells were measured by a gas-liquid chromatography using a column with a terephtalic acid support coated with polyethylene glycol-6000. The lactic acid contents were directly determined in aqueous samples, because they were converted to a...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(92)90407-g

    authors: Kageyama K,Onoyama Y,Nakajima T,Tumura M,Nishida T,Kogawa H,Tanabe K

    更新日期:1992-01-01 00:00:00

  • Expression of human alpha-fetoprotein in yeast.

    abstract::Human alpha-fetoprotein (AFP) was expressed in Saccharomyces cerevisiae, with a plasmid containing the cDNA sequence for human AFP fused with the rat AFP signal peptide. The recombinant AFP was purified from the yeast lysate by DEAE-cellulose and immunoaffinity chromatography. The amino acid composition and the molecu...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(90)90383-3

    authors: Yamamoto R,Sakamoto T,Nishi S,Sakai M,Morinaga T,Tamaoki T

    更新日期:1990-01-01 00:00:00

  • Mast cells: the immune gate to the brain.

    abstract::Mast cells were originally considered wandering histiocytes, but are now known to derive from the bone marrow and enter the tissues as immature or precursor cells which then differentiate under micro-environmental influences such as interleukin-3. At least three types of mature mast cells have been identified as seros...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/0024-3205(90)90129-f

    authors: Theoharides TC

    更新日期:1990-01-01 00:00:00

  • G(olf) protein levels in rat striatum are increased by chronic antidepressant administration and decreased by olfactory bulbectomy.

    abstract::There are many studies of the mechanisms of antidepressants; however, most of these studies were conducted on the hippocampus or frontal cortex. In the present study, we hypothesized that the nucleus accumbens and caudate/putamen might be major targets for antidepressant effects. Thus, we focused on G(olf) protein, a ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2006.01.033

    authors: Taoka H,Hamamura T,Endo S,Miki M,Lee Y,Miyata S,Toma K,Ishihara T,Sagara H,Gomita Y,Kuroda S

    更新日期:2006-06-27 00:00:00

  • The effects of calorie-matched high-fat diet consumption on spontaneous physical activity and development of obesity.

    abstract:AIMS:To characterize the effects of a calorie matched high-fat diet (HFD) on spontaneous physical activity (SPA), body weight, inflammatory status and expression of genes related to energy homeostasis in hypothalamus of mice. MAIN METHODS:C57Bl/6 mice (n=5 per group) were fed a control diet (16.5% calories from fat) -...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2017.04.017

    authors: Moretto TL,Benfato ID,de Carvalho FP,Barthichoto M,Le Sueur-Maluf L,de Oliveira CAM

    更新日期:2017-06-15 00:00:00

  • Anti-inflammatory action of dietary fish oil and calorie restriction.

    abstract::Inflammation, inflammatory mediators, cyclooxygenase (COX)-2, and inducible nitric oxide (iNOS) are all influenced by age-related oxidative status. To investigate the effect of dietary fish oil (FO) and calorie restriction (CR) on oxidative stress-related inflammatory status with age, (NZB/NZW) F1 (B/W) mice were fed ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2005.10.034

    authors: Kim YJ,Kim HJ,No JK,Chung HY,Fernandes G

    更新日期:2006-04-18 00:00:00

  • Two distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscle.

    abstract::Calcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in beta-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibiti...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(99)00152-6

    authors: Qian Y,Bourreau JP

    更新日期:1999-01-01 00:00:00

  • Therapeutic evaluation of solid lipid nanoparticle of quercetin in pentylenetetrazole induced cognitive impairment of zebrafish.

    abstract:BACKGROUND:Quercetin is a major flavonoid in various plants. It possesses the multiple pharmacological actions like vascular integrity and regulatory action of the blood-brain barrier. AIMS:The present study is focused on evaluating the role of solid lipid nanoparticle of quercetin in pentylenetetrazole (PTZ) induced ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2018.03.010

    authors: Rishitha N,Muthuraman A

    更新日期:2018-04-15 00:00:00

  • Alcohol-induced oxidative stress.

    abstract::Alcohol-induced oxidative stress is linked to the metabolism of ethanol involving both microsomal and mitochondrial systems. Ethanol metabolism is directly involved in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS). These form an environment favourable to oxidative stress. Ethanol ...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2007.05.005

    authors: Das SK,Vasudevan DM

    更新日期:2007-06-27 00:00:00

  • Induction of CYP3A4 by 1alpha,25-dyhydroxyvitamin D3 in HepG2 cells.

    abstract::CYP3A4, the predominant cytochrome P450 (CYP) expressed in human liver, contributes to the metabolism of approximately half the drugs in use today. In general, human-derived cell lines fail to express CYPs. It was previously shown that CYP3A4 mRNA and CYP3A immunoreactive protein are induced by 1alpha,25-dyhydroxyvita...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(03)00262-5

    authors: Elizondo G,Medina-Díaz IM

    更新日期:2003-05-30 00:00:00

  • Antidepressant-like behavioral and neurochemical effects of the citrus-associated chemical apigenin.

    abstract::Apigenin is one type of bioflavonoid widely found in citrus fruits, which possesses a variety of pharmacological actions on the central nervous system. A previous study showed that acute intraperitoneal administration of apigenin had antidepressant-like effects in the forced swimming test (FST) in ddY mice. To better ...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2008.01.007

    authors: Yi LT,Li JM,Li YC,Pan Y,Xu Q,Kong LD

    更新日期:2008-03-26 00:00:00

  • Nitrosamines as nicotinic receptor ligands.

    abstract::Nitrosamines are carcinogens formed in the mammalian organism from amine precursors contained in food, beverages, cosmetics and drugs. The potent carcinogen, NNK, and the weaker carcinogen, NNN, are nitrosamines formed from nicotine. Metabolites of the nitrosamines react with DNA to form adducts responsible for genoto...

    journal_title:Life sciences

    pub_type: 杂志文章,评审

    doi:10.1016/j.lfs.2007.03.006

    authors: Schuller HM

    更新日期:2007-05-30 00:00:00

  • Increased intracellular calcium activates serum and glucocorticoid-inducible kinase 1 (SGK1) through a calmodulin-calcium calmodulin dependent kinase kinase pathway in Chinese hamster ovary cells.

    abstract::SGK1 is one of the protein-serine/threonine kinases that is activated by insulin in a PI3K-dependent manner. Although SGK1 mediates a variety of biological activities, the mechanisms regulating its activity remain unclear. In this study, we examined the potential roles of calcium signaling in the activation of SGK1. T...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(03)00092-4

    authors: Imai S,Okayama N,Shimizu M,Itoh M

    更新日期:2003-04-04 00:00:00

  • Gestational exposure to excessive levels of dexamethasone impairs maternal care and impacts on the offspring's survival in rats.

    abstract::Administration of dexamethasone (DEX) during late gestation is a model to study growth restriction in rodents, but the pup's mortality index can be high, depending on DEX dosage, and little is known about the effects of DEX on maternal care (MC). Considering that an inadequate MC can also contribute to pup's mortality...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2020.118599

    authors: Cella EC,Conte J,Stolte RCK,Lorenzon F,Gregorio T,Simas BB,Rafacho A,Lima FB

    更新日期:2021-01-01 00:00:00

  • Central mu opioids mediate differential control of urine flow rate and urinary sodium excretion in conscious rats.

    abstract::Central administration of the selective mu opioid agonist, dermorphin, produces a concurrent diuretic and antinatriuretic response in conscious rats. To determine whether central mu opioids differentially affect the renal excretion of water and sodium, we examined changes in renal function produced by intracerebrovent...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(95)00065-e

    authors: Kapusta DR,Dzialowski EM

    更新日期:1995-01-01 00:00:00

  • Serotonin-mediated acute insulin resistance in the perfused rat hindlimb but not in incubated muscle: a role for the vascular system.

    abstract::We have recently shown that the vasoconstrictor serotonin (5-HT) inhibits oxygen uptake in perfused hindlimb possibly due to vascular shunting. Thus in the present study the effect of 5-HT on insulin-mediated glucose uptake was assessed. Rat hindlimbs were perfused at constant flow with medium containing 8.3 mM glucos...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(93)90563-i

    authors: Rattigan S,Dora KA,Colquhoun EQ,Clark MG

    更新日期:1993-01-01 00:00:00

  • Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats.

    abstract:AIMS:The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. MATERIAL AND METHODS:Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethan...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/j.lfs.2019.05.016

    authors: Rossetto IMU,Cagnon VHA,Lizarte FSN,Tirapelli LF,Tirapelli DPC,Arantes RMS,Chuffa LGA,Martinez FE,Martinez M

    更新日期:2019-07-15 00:00:00

  • Interaction of digoxin with antihypertensive drugs via MDR1.

    abstract::The multidrug transporter MDR1 (P-glycoprotein)-mediated interaction between digoxin and 29 antihypertensive drugs of various types was examined by using the MDR1 overexpressing LLC-GA5-COL150 cells, which were established by transfecting MDR1 cDNA into porcine kidney epithelial LLC-PK1 cells. These cells construct mo...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/s0024-3205(01)01494-1

    authors: Takara K,Kakumoto M,Tanigawara Y,Funakoshi J,Sakaeda T,Okumura K

    更新日期:2002-02-15 00:00:00

  • Prolonged beta-adrenoceptor stimulation increases the amount of GDP-binding protein in brown adipose tissue mitochondria.

    abstract::Cold acclimation increases the thermogenic capacity of brown adipose tissue (BAT) and this is associated with both a general hypertrophy of the tissue and a selective increase in the proportion of a specific 32,000 molecular weight, GDP-binding protein in the mitochondrial membrane. This protein regulates the activity...

    journal_title:Life sciences

    pub_type: 杂志文章

    doi:10.1016/0024-3205(84)90081-x

    authors: Young P,Wilson S,Arch JR

    更新日期:1984-03-19 00:00:00