Abstract:
:The effects of erythropoietin (EPO) on cytosolic free calcium concentration ([Ca2+]i) in platelets of 20 essential hypertensive patients (HT) and of 25 normotensive subjects (NT) were investigated using the fura2 technique. In resting platelets [Ca2+]i were not significantly higher in HT compared to NT (74.3 +/- 7.8 nM vs 59.8 +/- 7.0 nM, mean +/- SEM). Addition of EPO significantly increased [Ca2+]i in HT compared to NT (13.8 +/- 5.3 nM vs 0.9 +/- 1.9 nM, p less than 0.01). EPO increased the amount of calcium in intracellular stores. This was confirmed independently using thrombin-induced changes of [Ca2+]i in a calcium-free medium and using chlorotetracycline as a marker of stored calcium. After preincubation with EPO thrombin-induced changes of [Ca2+]i were significantly lower in HT compared to NT (306.1 +/- 30.0 nM vs 407.7 +/- 35.7 nM, p less than 0.05). In a calcium-free medium after preincubation with EPO thrombin-induced changes of [Ca2+]i were significantly lower in HT compared to NT (54.7 +/- 11.8 nM vs 100.9 +/- 10.5 nM, p less than 0.05) indicating lower storage capacity in HT. It is concluded that elevated response to EPO may provide a powerful tool to evaluate diagnosis and underlying pathophysiological mechanisms in essential hypertension.
journal_name
Life Scijournal_title
Life sciencesauthors
Tepel M,Wischniowski H,Zidek Wdoi
10.1016/0024-3205(92)90010-msubject
Has Abstractpub_date
1992-01-01 00:00:00pages
161-7issue
2eissn
0024-3205issn
1879-0631pii
0024-3205(92)90010-Mjournal_volume
51pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Several antihistamines were evaluated for their ability to interact with sigma, muscarinic and histaminic H1 binding sites in rat brain preparations. All of the antihistamines were able to interact with the sigma site, as well as the other two sites. In addition, tripelennamine was found to elicit sigma-like behaviors...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90231-f
更新日期:1990-01-01 00:00:00
abstract::Two male adult rhesus monkeys were used and caged individually. The room was temperature-controlled having a light-dark period of 12/12 hours. The animals were rapidly immobilized and immediately anesthetized with ketamine i. m. (10 mg/kg of body weight). They were bled four times at 15, 30, 45 and 60 mins after the k...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90247-9
更新日期:1991-01-01 00:00:00
abstract:AIMS:Resveratrol (Res) which is a polyphenolic phytoalexin, has various biological properties. In the present study, we investigated whether Res extracted from Polygonum cuspidatum can reduce oxidative damage and cognitive impairment in senescence-accelerated mouse (SAM). MAIN METHODS:Senescence-accelerated mice were ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.08.033
更新日期:2012-10-29 00:00:00
abstract:AIMS:LexA protein is a transcriptional repressor which regulates the expression of more than 60 genes belonging to the SOS global regulatory network activated by damages to bacterial DNA. Considering its role in bacteria, LexA represents a key target to counteract bacterial resistance: the possibility to modulate SOS r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117116
更新日期:2020-01-15 00:00:00
abstract::Previous studies have indicated that one of the causes of alveolar bone destruction with periodontitis is lipopolysaccharide (LPS) from the cell wall of gram-negative bacteria in plaque, and that prostaglandin E(2) (PGE(2)) is one of the bone resorption factors that stimulate osteoclast formation through an intercellu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.09.019
更新日期:2006-03-20 00:00:00
abstract::The density of bovine peripheral-type benzodiazepine receptors (PBR) in four tissues was highest in adrenal cortex. The adrenal cortex PBR cofractionated with a mitochondrial membrane marker enzyme and could be solubilized with intact ligand binding properties using digitonin. The membrane bound and soluble mitochondr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90090-x
更新日期:1991-01-01 00:00:00
abstract::The antinociceptive effect of the benzomorphan class of opioid analgesics have been difficult to measure utilizing some of the standard animal pain models. This may be due, in part, to the sedative and/or motor effects associated with these drugs. In addition, it has been proposed that the major site of action for dru...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90270-5
更新日期:1986-01-06 00:00:00
abstract:AIMS:The mechanism of cough hypersensitivity induced by particulate matter 2.5 (PM2.5) remains elusive. The current study was designed to explore the effect of transient receptor potential vanilloid-1 (TRPV1) on cough hypersensitivity in airway and central nervous system. MAIN METHODS:The PM2.5-induced chronic cough m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.02.064
更新日期:2016-04-15 00:00:00
abstract::Phosphatidylinositol (PI) specific phospholipase C treatment of rabbit platelets caused 95% release of acetylcholinesterase in the supernatant and 4 to 6% hydrolysis of membrane PI in 2 min. Under these conditions there was no cell lysis as monitored by lack of lactate dehydrogenase activity in the medium. The phospho...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90590-4
更新日期:1986-02-24 00:00:00
abstract::DHEA improves insulin sensitivity and has anti-obesity effect in animal models and men. However, the molecular mechanisms by which DHEA improves insulin action have not been clearly understood. In the present study, we examined the protein levels and phosphorylation state of insulin receptor (IR), IRS-1 and IRS-2, the...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.06.017
更新日期:2004-11-19 00:00:00
abstract::Intravenous (IV) administration of 10 ug/kg of morphine sulfate increased the firing rate of Type A dopamine neurons but decreased the extracellular activity of Type B dopamine cells in the substantia nigra pars compacta and ventral tegmental area of anesthetized male rats. The morphine-produced increase in Type A cel...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90153-9
更新日期:1982-11-15 00:00:00
abstract::Neurological diseases are becoming increasingly prominent worldwide due to rapidly aging populations, which greatly contributes to increasing healthcare costs. The development of neuroprotective drugs has so far proven exceptionally difficult due to the blood-brain barrier. One novel approach to address this challenge...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.12.025
更新日期:2018-02-15 00:00:00
abstract::The bone marrow is home to mesenchymal stem cells (MSCs) that are able to differentiate into many different cell types. The effect of hyperbaric oxygen (HBO) on MSCs is poorly understood. Placental growth factor (PlGF) is an attractive therapeutic agent for stimulating revascularization of ischemic tissue. HBO has bee...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.05.005
更新日期:2008-07-04 00:00:00
abstract::Identifying factors that modulate cocaine self-administration is fundamental to the development of effective strategies to treat and prevent cocaine abuse. In the present study, the influence of an alternative reinforcer on the probability of cocaine use was examined in four adult humans under controlled laboratory co...
journal_title:Life sciences
pub_type: 临床试验,杂志文章
doi:10.1016/0024-3205(94)00878-7
更新日期:1994-01-01 00:00:00
abstract::Acyl glucuronides are reactive electrophilic metabolites of carboxylate drugs, capable of undergoing hydrolysis, rearrangement and covalent binding reactions with proteins in vivo. Such covalent drug-protein adducts may be prerequisites for certain idiosyncratic immune and toxic responses in susceptible individuals. T...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00958-9
更新日期:2000-12-22 00:00:00
abstract::Repeated, low-intensity electrical stimulation of the heart gradually leads to the development of electrocardiographic abnormalities that culminate in cardiac arrhythmias, mainly A-V block in the isolated heart frog and ventricular and supraventricular tachyarrhythmias in the canine heart in situ. The pattern of devel...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90437-7
更新日期:1988-01-01 00:00:00
abstract::The behavioural and electrocortical (ECoG) effects of human recombinant tumor necrosis factor-alpha (hrTNF-alpha) and various forms of interleukin-1 (IL-1) microinjected into the locus coeruleus (LC) of rats were studied. IL-1 induced a typical, dose-dependent, behavioural sedation and/or sleep which was associated wi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00692-3
更新日期:1997-01-01 00:00:00
abstract:AIMS:To evaluate potential agents of therapeutic value in tissue inflammation, we studied norcantharidin (NCTD) and its derivatives for their effects on immune responses of human peripheral blood mononuclear cells (PBMC) in vitro. MAIN METHODS:PBMC proliferation was evaluated by tritiated thymidine uptake method. The ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.11.020
更新日期:2009-02-13 00:00:00
abstract::In adult male Wistar rats submitted to a standardized noise stress, intravenous TRH induced a prolactin (PRL) secretory response. Prior IV naloxone administration not only lowered plasma PRL levels in those stressed rats but abolished also the stimulatory action of TRH. This effect was further studied by superfusion e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90756-9
更新日期:1987-06-22 00:00:00
abstract::We studied the effect of interleukin-1 alpha (IL-1) on corticotropin-releasing hormone (CRH) secretion by explanted rat hypothalami in vitro. We also assessed possible mediation of arachidonic acid metabolites on IL-1-stimulated CRH secretion, by preincubating hypothalami with the cyclooxygenase inhibitor indomethacin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90190-3
更新日期:1990-01-01 00:00:00
abstract:AIMS:The aims of this study were to examine the role of endothelin-1 (ET-1), a pleiotropic peptide found at elevated levels in a number of malignancies and which has been shown to influence oral cancer cell behaviour via paracrine signalling pathways, on the phenotype of oral fibroblasts. MAIN METHODS:The effect of ET...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.04.001
更新日期:2012-10-15 00:00:00
abstract::The present investigation was aimed at comparing the influence of the partial dopamine (DA) receptor agonist (-)-3-PPP (preclamol) on prolactin release in acutely hyperprolactinemic but otherwise intact female rats and female rats subjected to chronic DA depletion. One group of animals received daily vehicle injection...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90100-2
更新日期:1988-01-01 00:00:00
abstract::We have previously reported that metronidazole treatment increases human lymphocyte proliferation showing individual differences. This drug and its metabolites are imidazole compounds like histamine and cimetidine. The first is an endogenous amine that inhibits T-helper lymphocyte proliferation, and the second is a hi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00297-4
更新日期:1996-01-01 00:00:00
abstract::An attenuation of adrenergic activity during the inhibition of endogenous angiotensin II formation was evaluated by determining plasma norepinephrine concentration after a single oral administration of captopril compared to that after nifedipine in essential hypertension. Captopril produced a fall in mean arterial pre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90415-8
更新日期:1991-01-01 00:00:00
abstract:AIMS:Osteoclasts, the unique bone-resorbing polykaryons, are responsible for many bone-destructive diseases, such as osteoporosis and rheumatoid arthritis. Hence, the regulation of osteoclast formation is considered a potential therapeutic approach for these diseases. In this study, we investigated the effect of a nove...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.09.009
更新日期:2012-11-02 00:00:00
abstract::NMDA receptors are glutamate-regulated ion channels of critical importance for many neurophysiological and neuropathological processes. Mg2+ blocks the NMDA receptor by binding to the channel pore with an apparent affinity that depends on the membrane potential. We have investigated the effect of NMDA and the required...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00975-4
更新日期:2001-03-09 00:00:00
abstract::P120-catenin (P120) was known to function in adhesion between cells and signal transduction in many types of cells. In this study, we investigated the expression and role of P120 in pulmonary fibrosis and transforming growth factor beta (TGF-β) mediated lung fibroblast-to-myofibroblast differentiation (fibroblast diff...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.05.052
更新日期:2019-08-01 00:00:00
abstract::Dopamine receptors in human parathyroid were studied in vitro using ligand binding techniques. With 3H-piflutixol as ligand, binding characteristic of the dopamine D1 receptor was observed. Administration of apomorphine, flupenthixol or metoclopramide to normal controls or acute schizophrenic patients at doses produci...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90779-8
更新日期:1983-08-22 00:00:00
abstract::Contractile responses to norepinephrine (NE), and the population of beta-adrenoceptors, were determined in gastric fundus smooth muscle from rats with diabetes induced by streptozotocin (STZ), and age-matched controls. Relaxation and/or contraction of fundus strips of controls and diabetics were induced by 10(-5)M NE....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90505-6
更新日期:1991-01-01 00:00:00
abstract::Neonatally androgenized (NA) female rats were ovariectomized (OVX) as adults and given 1 mg of testosterone propionate/day for 7 days and the plasma prolactin (PRL) pattern compared with NA intact animals and normal OVX animals given estrogen or TP. NA intact animals had elevated basal (morning values) and an attenuat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90663-4
更新日期:1982-04-05 00:00:00