Abstract：
:We have previously reported that metronidazole treatment increases human lymphocyte proliferation showing individual differences. This drug and its metabolites are imidazole compounds like histamine and cimetidine. The first is an endogenous amine that inhibits T-helper lymphocyte proliferation, and the second is a histamine antagonist. We presently report the in vitro effects of histamine, cimetidine, imidazole, metronidazole and its two principal metabolites (the acetic acid and hydroxy forms), on the mitogenic response to phytohemagglutinin (PHA) stimulation of human peripheral blood lymphocytes. Histamine decreased lymphocyte proliferation while (in order of potency) cimetidine, the hydroxy metabolite of metronidazole, imidazole and metronidazole, increased the mitogenic response to PHA in a dose-response fashion. The acetic acid metabolite lacked immunomodulatory effects. Competitive studies showed that cimetidine, metronidazole, and the hydroxy metabolite blocked the inhibitory effect of histamine on lymphocyte proliferation in a dose-related manner. This blockage was non-competitive, suggesting that the target of the imidazole compounds was not the active site of the H2 receptor.
journal_name
Life Scijournal_title
Life sciencesauthors
Elizondo G,Ostrosky-Wegman Pdoi
10.1016/0024-3205(96)00297-4subject
Has Abstractpub_date
1996-01-01 00:00:00pages
285-97issue
4eissn
0024-3205issn
1879-0631pii
0024320596002974journal_volume
59pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Nanoparticles (NPs)-based drugs have been recently introduced to improve the efficacy of current therapeutic strategies for the treatment of cancer; however, the molecular mechanisms by which a NP interacts with cellular systems still need to be delineated. Here, we utilize the autophagic potential of TiO2 NPs for...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117466
更新日期：2020-05-01 00:00:00
abstract：AIMS:Electromagnetic fields (EMFs) can act as inducers or mediators of stress response through the production of heat shock proteins (HSPs) that modulate immune response and thymus functions. In this study, we analyzed cellular stress levels in rat thymus after exposure of the rats to a 2.45 GHz radio frequency (RF) us...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.01.027
更新日期：2015-04-15 00:00:00
abstract：:In critically ill patients various conditions may lead to the activation of poly(ADP-ribose) polymerase (PARP). By promoting cellular energetic dysfunction, and by enhancing pro-inflammatory gene expression, PARP activation significantly contributes to the pathogenesis of shock. PARP activation is usually triggered by...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.11.001
更新日期：2008-01-16 00:00:00
abstract：BACKGROUND:Cigarette smoke extract (CSE) affects the expression of non-neuronal components of cholinergic system in bronchial epithelial cells and, as PEBP1/Raf-mediated MAPK1/2 and ERK1/2 pathway, promotes inflammation and oxidative stress. AIMS:We studied whether Acetylcholine (ACh) is involved in the mechanism of c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.11.034
更新日期：2018-01-01 00:00:00
abstract：:Muscarinic agonists elicit contraction in the standard guinea pig ileum bioassay through activation of M3 muscarinic receptors that are also linked to phosphoinositide hydrolysis. Surprisingly, the most abundant muscarinic receptor in the ileum is the M2 which causes a specific inhibition of cyclic AMP accumulation el...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(94)00035-q
更新日期：1995-01-01 00:00:00
abstract：AIM:The impact of the sympathetic nervous system (SNS) on the regulation of circadian rhythm and physiological functions is still not clear. Previous studies have found that essence of chicken (EC) supplementation facilitated the physiological adaption and circadian resetting in rats subjected to jet lag. Herein, the e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.03.047
更新日期：2018-05-15 00:00:00
abstract：AIMS:Aberrant expression of miRNAs exert the critical roles in carcinogenesis, including cervical cancer. Recent study corroborated the down-regulation of miR424-5p in uterine cervix adenocarcinoma. This research aimed to investigate the function and underlying mechanisms of miR424-5p in cervical cancer cell growth. M...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.01.006
更新日期：2017-02-15 00:00:00
abstract：AIMS:Tetramethylpyrazine (TMP), one of the active ingredients isolated from a Chinese herbal prescription, possesses protective effects against oxidative stress caused by high glucose in endothelial cells. In this study, the role of TMP in preventing muscle cells from palmitate-induced oxidative damage was investigated...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.02.025
更新日期：2011-04-25 00:00:00
abstract：:Iron imbalance has been implicated in oxidative injury associated with many brain diseases. The present study investigated the importance of iron location in hydroxyl radical (.OH) generation and the link between .OH production evaluated by the salicylate method and lipid peroxidation monitored by thiobarbituric acid-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00638-x
更新日期：2000-06-16 00:00:00
abstract：:Effects of lithium (Li) deficiency and/or immobilization stress on the thymus weight and differential blood count of rats were studied. The thymus weight of the rats fed a low Li diet were lighter than those of rats fed a Li supplemented diet, whether the rats were exposed to stress or not. Of the rats not exposed to ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00740-6
更新日期：1997-01-01 00:00:00
abstract：:Ipriflavone, 7-isopropoxy-3-phenyl-4H-1-benzopyran-4-one, was administered orally for 12 weeks to male rats with prednisolone-induced osteoporosis. Microdensitometric analysis of a roentgenograph of the femurs revealed that ipriflavone increased the density of the distal metaphysis dose-dependently and tended to incre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90265-1
更新日期：1986-03-10 00:00:00
abstract：:We investigated whether nitric oxide (NO) exposure alters the balance between NO and endothelium-derived hyperpolarizing factor (EDHF) released from rat renal arteries. To produce states of acutely or chronically excessive NO, lipopolysaccharide (LPS) was administered intraperitoneally to rats in a single dose of 4 mg...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.10.019
更新日期：2004-04-16 00:00:00
abstract：:G protein-coupled receptors (GPCRs) are integral membrane proteins that, in response to activation by extracellular stimuli, regulate intracellular second messenger levels via their coupling to heterotrimeric G proteins. GPCR activation also initiates a series of molecular events that leads to G protein-coupled recept...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2003.09.009
更新日期：2003-12-05 00:00:00
abstract：AIM:Betel-nut, a popular masticatory among Southeast Asian populations is a class I carcinogen, previously associated with dyslipidemia and aberrant lipid metabolism, and is reported to be used more frequently by females, than males. This study investigates the potential of repurposing the anti-diabetic drug, vildaglip...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118870
更新日期：2021-02-01 00:00:00
abstract：OBJECTIVE:We aimed to explore the expression level and biological function of miR-145-5p in preeclampsia (PE). METHODS:The differentially expressed miRNA/mRNA between normal placentas and PE placentas were screened using the GSE84260 and GSE73374 datasets from the Gene Expression Omnibus Database. The expression of mi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117008
更新日期：2019-12-15 00:00:00
abstract：AIM:The present study aimed to understand the relationship between pharmacological activation of mGlu4 receptors and regulation of its gene in the hippocampus. MAIN METHODS:The expression level of the GRM4 gene, encoding mGluR4 receptors, was studied in the hippocampus and frontal cortex of rats after pharmacological ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.03.033
更新日期：2016-05-15 00:00:00
abstract：:Liquid protein diet (LPD) has been shown previously to produce maternal and fetal weight loss and fetal congenital anomalies, including cataracts and craniofacial malformations. Therefore, to examine the effects of LPD in pregnancy upon the central nervous system of pups, pregnant dams were fed either a 20% casein die...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90867-6
更新日期：1983-04-04 00:00:00
abstract：:Ischemia-reperfusion of organs such as the kidney produces reactive oxygen and free radical species in tissues and leads to injury of intracellular molecules critical to cell homeostasis. Ischemia-reperfusion affects the NADPH-dependent monooxygenase system including P450 system, which is also a source of reactive oxy...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00604-2
更新日期：1997-01-01 00:00:00
abstract：:The interaction of cell surface hormone receptors with heterotrimeric G proteins is crucial for hormonal actions. The domains of the receptor, which interact with and activate G protein, have been extensively studied. However, precise molecular mechanisms underlying regulation of the receptor-induced G protein activat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.002
更新日期：2003-12-05 00:00:00
abstract：:Tiazofurin and retinoic acid synergistically induced differentiation and inhibited colony formation in HL-60 human promyelocytic leukemia cells in cell culture. The synergism was the result of different mechanisms of action, since the effect of tiazofurin, unlike that of retinoic acid, was prevented by addition of gua...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90087-8
更新日期：1990-01-01 00:00:00
abstract：AIMS:Morus, a member of the family Moraceae and commonly known as the mulberry, comprises a pharmaceutically important plant group whose major constituents are the moracins. Moracin derivatives have received great attention because they exhibit a diverse range of biological functionalities. However, no studies have con...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.08.060
更新日期：2018-10-01 00:00:00
abstract：AIMS:Circular RNAs (circRNAs) have been emerged as novel regulators in multiple tumorigenesis, including melanoma. CircRNA_0084043 was recently demonstrated to be deregulated in human melanoma cells. Nevertheless, its role and mechanism are largely unrevealed in melanoma. MATERIALS AND METHODS:Expression of circ_00840...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117323
更新日期：2020-02-15 00:00:00
abstract：:In fish liver catecholamines bind to beta-adrenoceptors (AR) and increase glucose release via cAMP augmentation. Alpha1-AR have recently been shown to mediate IP3 and Ca2+ elevation in catfish and eel hepatocytes, although their coupling to a physiological response has remained doubtful. We have perifused isolated cat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00216-7
更新日期：1999-01-01 00:00:00
abstract：:The effects of tetrandrine (TET) on the contractile responses of rat aortic rings and perfused rat mesenteric arteries to phenylephrine (PE) were investigated. TET inhibited the maximal contraction to PE in a concentration-dependent manner. TET significantly inhibited the transient contraction in Ca(2+)-free medium pr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00552-8
更新日期：1996-01-01 00:00:00
abstract：AIMS:This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.03.012
更新日期：2015-06-01 00:00:00
abstract：:Female mice of the inbred strains C57BL/6J, BALB/cJ and DBA/2J were used to determine the possible existence of a genetically-based differential susceptibility to the effects of treatment with an extract of Ginkgo biloba (EGb 761). Timm's silver-sulphide staining method was used to visualize and determine changes in t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00915-5
更新日期：1995-01-01 00:00:00
abstract：AIMS:The protein kinase C (PKC) family of enzymes has been implicated in cellular proliferation, differentiation, and apoptosis. However, the distribution of specific PKC isoforms with varying functions in normal and malignant human tissues remains to be determined. The objective of this study was to investigate the ex...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.03.007
更新日期：2009-05-22 00:00:00
abstract：AIMS:The aim of the present study was to characterize TRPV4 channels in pregnant and nonpregnant mouse uterus and examine their functional role in spontaneous and agonist-induced contractions. MAIN METHODS:We used RT-PCR, Western blot and immunohistochemistry experiments to demonstrate the presence of TRPV4 mRNA and p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.12.010
更新日期：2015-02-01 00:00:00
abstract：:[3H]A-69024 has been prepared as a radioligand for studying the dopamine D1 receptor. [3H]A-69024 binds to rat striatal membranes with a KD = 14.3 +/- 3.2 nM (mean +/- SEM; n = 3) and Bmax = 63.5 +/- 12.8 fmol/mg wet tissue (1.8 +/- 0.3 pmol/mg protein). This ligand binds to only one site with a Hill coefficient close...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02212-2
更新日期：1995-01-01 00:00:00
abstract：:New types of alpha1-adrenoceptor antagonists (tamsulosin, KMD-3213 and JTH-601) are currently receiving a great deal of attention, especially in terms of developing effective therapeutic agents to treat bladder outlet obstruction with less side effects, such as postural hypotension, in patients with benign prostatic h...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00111-8
更新日期：1998-01-01 00:00:00
