Abstract:
:DHEA improves insulin sensitivity and has anti-obesity effect in animal models and men. However, the molecular mechanisms by which DHEA improves insulin action have not been clearly understood. In the present study, we examined the protein levels and phosphorylation state of insulin receptor (IR), IRS-1 and IRS-2, the association between IRSs and PI3K and SHP2, the insulin-induced IRSs associated PI 3-kinase activities, and the phosphorylation status of AKT and atypical PKCzeta/lambda in the liver and the muscle of 6 month-old Wistar rats treated with DHEA. There was no change in IR, IRS-1 and IRS-2 protein levels in both tissues of treated rats analysed by immunoblotting. On the other hand, insulin-induced IRS-1 tyrosine phosphorylation was increased in both tissues while IRS-2 tyrosyl phosphorylation was increased in liver of DHEA treated group. The PI3-kinase/AKT pathway was increased in the liver and the PI3K/atypical PKCzeta/lambda pathway was increased in the muscle of DHEA treated rats. These data indicate that these regulations of early steps of insulin action may play a role in the intracellular mechanism for the improved insulin sensitivity observed in this animal model.
journal_name
Life Scijournal_title
Life sciencesauthors
Campbell CS,Caperuto LC,Hirata AE,Araujo EP,Velloso LA,Saad MJ,Carvalho CRdoi
10.1016/j.lfs.2004.06.017subject
Has Abstractpub_date
2004-11-19 00:00:00pages
57-70issue
1eissn
0024-3205issn
1879-0631pii
S0024-3205(04)00779-9journal_volume
76pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Hyperhomocysteinemia (Hhcy) is an independent risk factor of atherosclerosis and promotes unstable plaque formation. Epigenetic mechanisms play an important role in the pathogenesis of atherosclerosis induced by Hhcy. However, the exact mechanism is still undefined. Lesional apoptotic cells and necrotic core forma...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.02.003
更新日期:2017-03-15 00:00:00
abstract::Using liposomes differing in size and lipid composition, we have studied the uptake characteristics of the liver parenchymal and Kupffer cells. Desferal labeled with iron-59 was chosen as a radiomarker for the liposomal content, because Desferal in its free form does not cross cellular membranes. At various time inter...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90098-4
更新日期:1982-11-08 00:00:00
abstract::The reproductive system effects of cocaine were studied in male rats. The analysis included measurements of circulating levels of luteinizing hormone (LH) and testosterone (T) by radioimmunoassay (RIA). The weights of the testes and sex accessory organs were also assessed and compared with control animals. Dosage leve...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90440-2
更新日期:1989-01-01 00:00:00
abstract::Pregnant mice are more susceptible to flurothyl-induced seizures than are non-pregnant controls. The possibility that the well-known increase in beta-endorphin concentration which accompanies pregnancy was involved in this effect was examined by testing whether naloxone administration could block the increased seizure...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90218-3
更新日期:1986-05-26 00:00:00
abstract::Hyperglycemia and oxidative stress are the primary stressors that elicit mitochondria specific cell stress in diabetes. Here we hypothesized that elevated level of ROS in high glucose (HG) environment, trigger mitochondrial stress by damaging mitochondrial DNA (mtDNA), altering inflammatory mediators, and neurodegener...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117914
更新日期:2020-09-01 00:00:00
abstract:AIMS:The role of estrogen in embryo implantation in golden hamster (Mesocricetus auratus) is still ambiguous. In order to clarify it, we investigated the spatial distribution and expression of estrogen receptors, ER-α and ER-β in the uterus of pregnant hamster during peri-implantation period and identified the effect o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.12.002
更新日期:2017-02-01 00:00:00
abstract:AIMS:Fudosteine is a cysteine derivative that is used as an expectorant in chronic bronchial inflammatory disorders. It has been shown to decrease the number of goblet cells in an animal model. This study examined the effects of fudosteine on airway inflammation and remodeling in a murine model of chronic asthma. MAIN...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.03.022
更新日期:2013-05-30 00:00:00
abstract::Using a specific radioimmunoassay, we measured concentrations of plasma 7B2 (a novel pituitary polypeptide) immunoreactivity (7B2-IR) in normal human subjects, patients with chronic renal failure and those with liver cirrhosis. Mean (+/- SEM) values of plasma 7B2-IR in normal healthy men and women were 55.8 +/- 1.2 pg...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90685-0
更新日期:1987-08-24 00:00:00
abstract::A previously unreported anti-bacterial activity against Escherichia coli has been found in porcine and bovine sera, but not in human or chicken sera. The activity is not affected by heat inactivation of the complement system, and is bacteriostatic rather than bactericidal. Growth inhibition is reversible by addition o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00277-d
更新日期:1995-01-01 00:00:00
abstract::The existence of a new factor (AF) in mice acting synergistically with known proaggregatory stimuli has been suggested by the present study in the plasma of mice challenged with intravenous collagen and adrenaline. Indomethacin, nordihydroguaretic acid (NDGA), BW 755C, phentolamine, cimetidine and ketanserin could not...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90224-f
更新日期:1990-01-01 00:00:00
abstract::Resveratrol, a polyphenolic substance found in grape skin, is proposed to account in part for the protective effect of red wine in the cardiovascular system. The aim of the present study is to investigate the action and possible mechanisms of resveratrol-produced regulation of plasma glucose in normal and diabetic rat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.02.002
更新日期:2007-04-10 00:00:00
abstract::Previous investigations showed that chronic metabolic acidosis (CMA) increased the paracellular permeability of ion and neutral hydrophilic molecules in the duodenum of rats and small intestinal-like cell lines. Since proteins of the claudin family have been known to regulate the paracellular transport in several epit...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.01.063
更新日期:2007-04-17 00:00:00
abstract::Beriberi is a disease caused by thiamine deficiency (TD), which may lead to heart problems, including heart failure. Despite the fact that thiamine prevalence is reduced in the industrialized world, it remains a health hazard especially due to chronic alcohol consumption. Diagnosing the presence of TD based on both el...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2013.12.029
更新日期:2014-03-07 00:00:00
abstract::The effects of chronic treatment with antidepressants, verapamil, or lithium on serotonin (5-HT)-induced Ca2+ increase were investigated in single C6BU-1 glioma cells with digital imaging microscopy. Clomipramine and citalopram, at a concentration of 100 nM, decreased the peak values of 5-HT-induced [Ca2+]i changes. V...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00010-6
更新日期:1997-01-01 00:00:00
abstract:AIMS:Schisandra is a good choice in Traditional Chinese Medicine for the therapy of cardiovascular diseases, but whether it contains a or some specific component (s) responsible these effects are still unclear. In the present study, we explored whether Schisantherin A (SCA) causes vasorelaxation in isolated rat thoraci...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117357
更新日期:2020-03-15 00:00:00
abstract::The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.02.023
更新日期:2006-07-10 00:00:00
abstract::Responses to [Mpr14]-ADM(14-50), a novel analog of adrenomedullin, were investigated in the hindlimb vascular bed of the cat under conditions of controlled blood flow. Intraarterial injections of [Mpr14]-rADM(14-50) in doses of 0.003-1 nmol caused dose-related decreases in hindlimb perfusion pressure. In terms of rela...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00258-5
更新日期:1996-01-01 00:00:00
abstract::The effects of the tricyclic antidepressant imipramine (20 mg/kg) and the beta-adrenoceptor agonist clenbuterol (0.5 mg/kg) on the serum concentrations of tyrosine, tryptophan, glucose and insulin were compared 30 min after intraperitoneal injection. The drugs had nearly identical effects on serum tyrosine, which was ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90621-w
更新日期:1990-01-01 00:00:00
abstract::The concentrations of the trace amines, m-tyramine, p-tyramine, phenylethylamine and tryptamine, were measured in the striatum of the brain and in the kidney of adult rats treated with alpha-monofluoromethyldopa (MFMD), an inhibitor of aromatic amino acid decarboxylase. While MFMD decreased the levels of all four amin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90371-7
更新日期:1987-02-09 00:00:00
abstract:AIMS:Aging and drug-induced side effects may contribute to deteriorate mitochondrial bioenergetics in many tissues, including kidney and liver. One possibility is that the combination of both aging and drug toxicity accelerates the process of mitochondrial degradation, leading to progressive bioenergetic disruption. We...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.07.004
更新日期:2013-09-03 00:00:00
abstract::The adverse effects of prenatal nicotine and alcohol exposure on human reproductive outcomes are a major scientific and public health concern. In the United States, substantial percentage of women (20-25%) of childbearing age currently smoke cigarettes and consume alcohol, and only a small percentage of these individu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.12.010
更新日期:2018-02-01 00:00:00
abstract::This study was designed to document possible changes in bupivacaine and its main metabolite, des-butyl-bupivacaine (PPX) kinetics in mice after a single 5 mg/kg injection of diltiazem. After a single 20 mg/kg i.p. dose of bupivacaine, kinetic parameters of bupivacaine were not statistically different but the ratio AUC...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00601-6
更新日期:1994-01-01 00:00:00
abstract::This study assessed the effects of acute intravenous L-tryptophan (neutral amino acid precursor for serotonin) administration on cocaine-induced dopaminergic responses. Male Sprague-Dawley rats were surgically implanted with guide cannulas in the nucleus accumbens 5 days prior to the study and with vascular catheters ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01276-0
更新日期:2001-09-07 00:00:00
abstract::Amniotic fluid and placenta contain a substance (POEF, for Placental Opioid-Enhancing Factor) that, when ingested, enhances opioid-mediated analgesia in nonpregnant rats; ingestion of the substance by rats not experiencing opioid-mediated analgesia, however, does not produce analgesia. It is highly likely that peripar...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90074-2
更新日期:1990-01-01 00:00:00
abstract::The effects of longer lasting blood pressure changes on the release of endogenous catecholamines (CA) in limbic and hypothalamic areas were studied in anaesthetized rats. For this purpose the central nucleus of the amygdala (AC), ventral hippocampus (VH) and medial hypothalamus (MH) were simultaneously superfused thro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90496-6
更新日期:1987-07-13 00:00:00
abstract::Tobacco smoke (TS) is a potent source of oxidants and oxidative stress is an important mechanism by which TS exerts its toxicity in the lung. We have shown that TS induces heat shock (HS)/stress protein (HSP) synthesis in human monocytes. Pulmonary surfactant (PS) whose major physiological function is to confer mechan...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00542-6
更新日期:1999-01-01 00:00:00
abstract:AIM:This study aimed to evaluate the effects of Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), on aortic hyporeactivity to Phenylephrine (Phe) and nitric oxide bioavailability associated with pregnancy in hypertensive rats. MAIN METHODS:The intact aortic rings of pregnant and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.12.014
更新日期:2015-02-01 00:00:00
abstract::Ventricular hypertrophy is a risk factors for arrhythmias, ischemia and sudden death. It involves cellular modifications leading to a pathological remodeling and is associated with heart failure. The activation of the G protein-coupled estrogen receptor (GPER) mediates beneficial actions in the cardiovascular system. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117211
更新日期:2020-02-01 00:00:00
abstract::Neuroblastoma x glioma hybrid cells NG 108-15 were chronically treated with opioid agonists and tested for their development of "tolerance" as evaluated by the loss of inhibitory activity of opioids upon cAMP-accumulation. Desensitization was dose-dependent and non-competitive and highly selective, since the activity ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90512-x
更新日期:1983-01-01 00:00:00
abstract::Nitric oxide (NO) is present in the exhaled breath of humans and experimental animals, but its physiologic role and cellular source(s) remain to be determined. Possible sites of origin are pulmonary endothelial cells and/or resident macrophages. Here we have tested the hypothesis that changes in cardiovascular status ...
journal_title:Life sciences
pub_type: 临床试验,杂志文章
doi:10.1016/0024-3205(94)00522-2
更新日期:1994-01-01 00:00:00