Abstract：
:We have developed an assay for serotonin (5-HT) stimulation of adenylate cyclase activity in membranes from adult guinea pig hippocampus. The response to 5-HT is concentration-dependent, with an EC50 of 0.01 microM, a shallow slope, and mean maximal stimulation of 90% over basal activity. The response to 5-HT is GTP-dependent and additive to the maximal stimulation by histamine. Micromolar concentrations of the known 5-HT receptor agonists, tryptamine and 5-methoxytryptamine, also stimulate cAMP production in this system, and their effect is not additive to that elicited by a maximal concentration of 5-HT. These results are consistent with the hypothesis that the response to 5-HT is elicited through a distinct receptor coupled to adenylate cyclase; the magnitude and the reproducibility of the 5-HT response in this system should make it useful for receptor classification. To examine the effect of prior exposure to endogenous 5-HT on the responsiveness of the system, we assayed 5-HT stimulation of enzyme activity in membranes prepared from animals 25-27 hrs after treatment with a single injection of reserpine (5 mg/kg, i.p.). The mean maximal stimulation of adenylate cyclase by 5-HT was increased to 150% over basal activity with no effect on the EC50 or slope of the 5-HT concentration-response curve. Reserpine pretreatment did not affect basal activity or histamine-stimulated adenylate cyclase activity. These results are discussed in the context of a hypothesis that endogenous 5-HT normally exerts a desensitizing effect on its receptors in situ.
journal_name
Life Scijournal_title
Life sciencesauthors
Shenker A,Maayani S,Weinstein H,Green JPdoi
10.1016/0024-3205(83)90763-4subject
Has Abstractpub_date
1983-05-16 00:00:00pages
2335-42issue
20eissn
0024-3205issn
1879-0631pii
0024-3205(83)90763-4journal_volume
32pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Oncogenic RET/PTC1 chromosomal rearrangements are hallmarks of thyroid papillary carcinoma. The resulting protein, mainly through tyrosine 451, is responsible for the activation of pathways controlling cell survival, including the PI3K/Akt/mTOR cascade. Vanadium compounds were shown to have anti-neoplastic potenti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.07.004
更新日期：2011-09-12 00:00:00
abstract：:Diesel particles form a large component of the fine particle fraction (PM10) in urban air in the UK. During pollution episodes small increases in PM10 have been linked to detrimental health effects. The comparative toxicological effects of diesel exhaust and other well-characterised particles (carbon black, amorphous ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00141-6
更新日期：1998-01-01 00:00:00
abstract：AIM:Exosomes, the nano-units (<200 nm), released from diverse cell types in the extracellular body fluid, possess non-immunogenic property and ability to cross the blood-brain barrier (BBB). Since exosomes carry biological information from their cells of origin, we hypothesize that priming cells with potential therapeu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.04.018
更新日期：2014-06-27 00:00:00
abstract：:To understand the leptin's action on hypothalamic arcuate nucleus (ARC), Ca2+/calmodulin (CaM)-dependent response in leptin-treated rat ARC was investigated. The in vitro phosphorylation of ARC extracts from leptin-treated rats did not respond to CaM. Although CaM-dependent protein kinase II activity was not affected ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00460-3
更新日期：1998-01-01 00:00:00
abstract：:Alpha2-adrenoceptor (alpha2-AR)-regulated binding of the labelled GTP analog, guanosine 5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS), to guanine-nucleotide-binding proteins (G proteins) was studied in human platelet membranes. Under optimal conditions, the potent alpha2-AR agonist, 5-bromo-6-(2-imidazolin-2-ylamino...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00074-0
更新日期：1999-01-01 00:00:00
abstract：:An increase in the weight of the thyroid gland, accompanied by reductions in uptake and release of 1311, serum T3 and reverse T3 concentrations and an increase in serum TSH concentration, occurs during the development of renal hypertension in rats. Renal function, as assessed by BUN, serum and urinary creatinine conce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90274-0
更新日期：1982-02-15 00:00:00
abstract：AIMS:Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression. The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins. MAIN METHODS:Ectosomes released by primary (WM1...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.06.026
更新日期：2018-08-15 00:00:00
abstract：:Electrically evoked release of dopamine from the caudate nucleus is reduced by the dopamine receptor agonists, apomorphine and bromocriptine, and facilitated by neuroleptic drugs, which act as dopamine autoreceptor antagonists. The potencies of chlorpromazine, fluphenazine, levomepromazine and their hydroxy-metabolite...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90088-9
更新日期：1990-01-01 00:00:00
abstract：:Although renin-angiotensin system (RAS) imbalance is manifested in cardiomyopathies with different etiologies, the impact of RAS effectors on Chagas cardiomyopathy and skeletal myositis is poorly understood. Given that diminazene aceturate (DMZ) shares trypanocidal, angiotensin-converting enzyme 2 (ACE2) and angiotens...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118067
更新日期：2020-09-15 00:00:00
abstract：:The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.023
更新日期：2006-07-10 00:00:00
abstract：:The plasma membrane ATPase on the human umbilical vein endothelial cell line (ECV304) was demonstrated to be an ecto-enzyme. Hydrolysis of ATP was measured by monitoring the appearance of inorganic phosphorus. Hydrolysis of extracellular ATP was insensitive to oligomycin, vanadate, ouabain and N-ethylmaleimide, compou...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02004-3
更新日期：1995-01-01 00:00:00
abstract：:Immunoreactive atrial natriuretic peptide (ir-ANP) was measured in the follicular fluid of pig ovarian follicle, and rabbit ovarian homogenates and perfusates using a specific radioimmunoassay (RIA). Serial dilution curves made with the extracts of follicular fluid, ovarian homogenates and perfusates using SepPak C18 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90425-6
更新日期：1989-01-01 00:00:00
abstract：:There are many studies of the mechanisms of antidepressants; however, most of these studies were conducted on the hippocampus or frontal cortex. In the present study, we hypothesized that the nucleus accumbens and caudate/putamen might be major targets for antidepressant effects. Thus, we focused on G(olf) protein, a ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.01.033
更新日期：2006-06-27 00:00:00
abstract：AIMS:Endothelin-1 (ET-1) and endothelin-2 (ET-2; Trp(6)Leu(7)ET-1) are expressed by different cell types, but are considered to display identical pharmacological properties on endothelin receptors. We studied agonist-dependent aspects of endothelin(A) (ET(A))-receptor function and the importance of amino acids 6 and 7 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.02.011
更新日期：2012-10-15 00:00:00
abstract：:Fluorescence spectroscopy was used to examine the biophysical characteristics of human platelet membranes as a function of subject age. The structural order of membrane lipid domains was determined with the use of 1,6-diphenyl-1,3,5-hexatriene (DPH), a fluorescent probe that preferentially localizes in the hydrocarbon...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90079-7
更新日期：1985-10-14 00:00:00
abstract：AIMS:To investigate the effects of aerobic exercise training on cardiomyocyte ultrastructure, oxidative stress, and activation of protein synthesis pathways in a model of cardiomyopathy induced by doxorubicin (Dox). MAIN METHODS:Male Sprague Dawley rats were randomly assigned to Control (saline, sedentary), Dox/sedent...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118698
更新日期：2021-01-01 00:00:00
abstract：:Groups of 35-day-old male C57BL/6 mice were stressed 1 hour per day by immobilization for 1, 2, 3, 5, 8, 11 or 14 consecutive days. Control groups were left undisturbed. The animals were then killed and body weight and the weights of the thymus, spleen and axillary lymph nodes determined. Chronic immobilization stress...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00606-1
更新日期：1997-01-01 00:00:00
abstract：:Rats were administered intravenously liposome-encapsulated or free cobalt, and the organ distribution of the metal was explored using Co57 tracer. Two hours after administration, the cobalt level in the heart was about 40% of the control when given in sphingomyelin (SM)/cholesterol (CH) (1:1 mole ratio) liposomes. The...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90092-1
更新日期：1989-01-01 00:00:00
abstract：:P75(NTR) is a common neurotrophin receptor which binds all neurotrophins with similar affinities and has been shown to be capable of mediating programmed cell death. In this study, we investigated effects of the antipsychotic drugs (APDs) haloperidol, clozapine, quetiapine, and risperidone on p75(NTR) mRNA levels in P...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.01.039
更新日期：2006-07-04 00:00:00
abstract：:In this study we analyzed the N-formyl-Met-Leu-Phe (fMLP)-induced calcium signal in alveolar macrophages (AM) isolated from ovalbumin-sensitized (OA-sensitized AM) and naive (naive AM) guinea-pigs. Guinea-pigs were sensitized by subcutaneous injection of OA and AM were isolated by bronchoalveolar lavage 6 weeks therea...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01239-5
更新日期：2001-08-24 00:00:00
abstract：:We investigated the involvement of alpha 1-adrenoceptor subtypes in the positive chronotropic response to norepinephrine (NE) in neonatal rat cardiac myocytes at day 3 of culture. The cardiac myocytes at day 3 of culture exhibited a dose-dependent positive chronotropic response to NE in the presence of propranolol, a ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90155-4
更新日期：1994-01-01 00:00:00
abstract：:We reported recently that ventromedial hypothalamic (VMH) lesions increased the synthesis of DNA in the gastrointestinal tract of rats by the firing of vagus nerve activity, mainly via cholinergic receptor mechanisms. In the present study, we examined whether the mitotic response is due to proliferation of a cell popu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02014-a
更新日期：1995-01-01 00:00:00
abstract：:We developed an experimental model to study the effect of daily cocaine administration on the mucosal immune system during murine acquired immune deficiency syndrome (MAIDS). Mice were infected with LP-BM5 murine leukemia virus, a retrovirus which causes immunosuppression with development of functional murine AIDS. Mi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00874-4
更新日期：1994-01-01 00:00:00
abstract：:Activin A is a member of the transforming growth factor-beta superfamily that exerts its diverse biological effects through bindings to activin specific transmembrane serine/threonine kinase receptors. The fibroblast-mediated contraction of a collagen gel is thought to be a model of part of the wound-repair response a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00480-x
更新日期：2000-03-01 00:00:00
abstract：:Calcitonin secretion is influenced by estrogen. In the present study, basal and calcium-stimulated serum calcitonin levels were reduced in ovariectomized rats, and replacement estrogen administered for 3 weeks increased both calcitonin levels to those in intact rats. Ipriflavone, 7-isopropoxy-3-phenyl-4H-1-benzopyran-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90492-3
更新日期：1986-04-28 00:00:00
abstract：:The purpose of this study was to examine whether the adhesion of polymorphonuclear leukocytes (PMNs) to endothelial cells and/or reactive oxygen species (ROS) released from PMNs are responsible for inducing angiogenesis. Angiogenesis was assessed by tube formation using endothelial cells obtained from bovine thoracic ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00537-3
更新日期：2000-04-14 00:00:00
abstract：AIMS:Sodium nitrite is used to inhibit the growth of microorganisms and is responsible for the desirable red color of meat; however, it can be toxic in high quantities for humans and other animals. Moreover, glycogen, a branched polysaccharide, efficiently stores and releases glucose monosaccharides to be accessible fo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.11.002
更新日期：2015-01-01 00:00:00
abstract：:Alpha(1)-adrenergic receptors are one of the three subfamilies of G protein coupled receptors activated by epinephrine and norepinephrine to control important functions in many target organs. Three human subtypes (alpha(1A), alpha(1B), alpha(1D)) are derived from separate genes and are highly homologous in their trans...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2003.07.008
更新日期：2003-12-12 00:00:00
abstract：AIMS:The potential of human mesenchymal stem cell-like stroma prepared from placental/umbilical cord blood for hematopoietic regeneration by X-irradiated hematopoietic stem cells is herein assessed. MAIN METHODS:Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells were applied to a regenerat...
journal_title：Life sciences
pub_type： 杂志文章
doi：
更新日期：2009-04-24 00:00:00
abstract：:The effect of clotrimazole on Ca2+ signaling in Madin Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ indicator. Clotrimazole (1-30 microM) induced a concentration-dependent [Ca2+]i increase. The [Ca2+]i increase comprised an initial rise and a slow decay. External Ca2+ removal partly inhib...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00558-0
更新日期：2000-01-01 00:00:00
